Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen

Producción Científica

Detalles Bibliográficos
Autores: De La Fuente Ballesteros, Adrián, Augé, Camille, Bernal del Nozal, José, Ares Sacristán, Ana María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/63739
Acceso en línea:https://doi.org/10.3390/molecules28062497
https://uvadoc.uva.es/handle/10324/63739
Access Level:acceso abierto
Palabra clave:Acaricide
Pesticides
Bee pollen
Polen
Abejas
Gas chromatography
Cromatografía de gases
Mass spectrometry
Methodology - Validity
Sample treatment
Analytical chemistry
Spectroscopy
2301 Química Analítica
3101.09 Plaguicidas
2209.21 Espectroscopia
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spelling Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollenDe La Fuente Ballesteros, AdriánAugé, CamilleBernal del Nozal, JoséAres Sacristán, Ana MaríaAcaricidePesticidesBee pollenPolenAbejasGas chromatographyCromatografía de gasesMass spectrometryMethodology - ValiditySample treatmentAnalytical chemistrySpectroscopy2301 Química Analítica3101.09 Plaguicidas2209.21 EspectroscopiaProducción CientíficaPesticides can be found in beehives for several reasons, including contamination from surrounding crops or for their use by beekeepers, which poses a risk to bee ecosystems and consumers. Therefore, efficient and sensitive methods are needed for determining pesticide residues in bee products. In this study, a new analytical method has been developed and validated to determine seven acaricides (atrazine, chlorpyrifos, chlorfenvinphos, α-endosulfan, bromopropylate, coumaphos, and τ-fluvalinate) in bee pollen using gas chromatography coupled to mass spectrometry. After an optimization study, the best sample treatment was obtained when using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method employing an ethyl acetate and cyclohexane as the extractant mixture, and a mixture of salts for the clean-up step. A chromatographic analysis (<21 min) was performed in an Agilent DB-5MS column, and it was operated under programmed temperature conditions. The method was fully validated in terms of selectivity, limits of detection (0.2–3.1 µg kg−1) and quantification (0.6–9.7 µg kg−1), linearity, matrix effect (<20% in all cases), trueness (recoveries between 80% and 108%), and precision. Finally, the proposed method was applied to analyze commercial bee pollen samples, and some of the target pesticides (chlorfenvinphos, α-endosulfan, coumaphos, and τ-fluvalinate) were detected.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) y Fondo Europeo de Desarrollo Regional (FEDER) - (grant RTA2017-00004-C02-02MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3390/molecules28062497https://uvadoc.uva.es/handle/10324/63739reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.mdpi.com/1420-3049/28/6/2497info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/637392026-06-13T12:44:47Z
dc.title.none.fl_str_mv Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
title Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
spellingShingle Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
De La Fuente Ballesteros, Adrián
Acaricide
Pesticides
Bee pollen
Polen
Abejas
Gas chromatography
Cromatografía de gases
Mass spectrometry
Methodology - Validity
Sample treatment
Analytical chemistry
Spectroscopy
2301 Química Analítica
3101.09 Plaguicidas
2209.21 Espectroscopia
title_short Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
title_full Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
title_fullStr Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
title_full_unstemmed Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
title_sort Development and validation of a gas chromatography-mass spectrometry method for determining acaricides in bee pollen
dc.creator.none.fl_str_mv De La Fuente Ballesteros, Adrián
Augé, Camille
Bernal del Nozal, José
Ares Sacristán, Ana María
author De La Fuente Ballesteros, Adrián
author_facet De La Fuente Ballesteros, Adrián
Augé, Camille
Bernal del Nozal, José
Ares Sacristán, Ana María
author_role author
author2 Augé, Camille
Bernal del Nozal, José
Ares Sacristán, Ana María
author2_role author
author
author
dc.subject.none.fl_str_mv Acaricide
Pesticides
Bee pollen
Polen
Abejas
Gas chromatography
Cromatografía de gases
Mass spectrometry
Methodology - Validity
Sample treatment
Analytical chemistry
Spectroscopy
2301 Química Analítica
3101.09 Plaguicidas
2209.21 Espectroscopia
topic Acaricide
Pesticides
Bee pollen
Polen
Abejas
Gas chromatography
Cromatografía de gases
Mass spectrometry
Methodology - Validity
Sample treatment
Analytical chemistry
Spectroscopy
2301 Química Analítica
3101.09 Plaguicidas
2209.21 Espectroscopia
description Producción Científica
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/molecules28062497
https://uvadoc.uva.es/handle/10324/63739
url https://doi.org/10.3390/molecules28062497
https://uvadoc.uva.es/handle/10324/63739
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/1420-3049/28/6/2497
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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