Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis

13 Pág.

Detalles Bibliográficos
Autores: Abril-Urías, Patricia, Ruiz-Ferrer, Virginia, Cabrera Chaves, Javier, Olmo, Rocio, Silva, Ana Cláudia, Díaz-Manzano, Fernando Evaristo, Domínguez-Figueroa, José, Martínez-Gómez, Ángela, Gómez-Rojas, Almudena, Moreno-Risueño, Miguel Ángel, Fenoll, Carmen, Escobar, Carolina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/311758
Acceso en línea:http://hdl.handle.net/10261/311758
https://api.elsevier.com/content/abstract/scopus_id/85149493267
Access Level:acceso abierto
Palabra clave:Auxin regulated genes
Galls
Giant cells
Lateral root formation
Syncytia
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oai_identifier_str oai:digital.csic.es:10261/311758
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
title Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
spellingShingle Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
Abril-Urías, Patricia
Auxin regulated genes
Galls
Giant cells
Lateral root formation
Syncytia
title_short Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
title_full Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
title_fullStr Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
title_full_unstemmed Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
title_sort Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in Arabidopsis
dc.creator.none.fl_str_mv Abril-Urías, Patricia
Ruiz-Ferrer, Virginia
Cabrera Chaves, Javier
Olmo, Rocio
Silva, Ana Cláudia
Díaz-Manzano, Fernando Evaristo
Domínguez-Figueroa, José
Martínez-Gómez, Ángela
Gómez-Rojas, Almudena
Moreno-Risueño, Miguel Ángel
Fenoll, Carmen
Escobar, Carolina
author Abril-Urías, Patricia
author_facet Abril-Urías, Patricia
Ruiz-Ferrer, Virginia
Cabrera Chaves, Javier
Olmo, Rocio
Silva, Ana Cláudia
Díaz-Manzano, Fernando Evaristo
Domínguez-Figueroa, José
Martínez-Gómez, Ángela
Gómez-Rojas, Almudena
Moreno-Risueño, Miguel Ángel
Fenoll, Carmen
Escobar, Carolina
author_role author
author2 Ruiz-Ferrer, Virginia
Cabrera Chaves, Javier
Olmo, Rocio
Silva, Ana Cláudia
Díaz-Manzano, Fernando Evaristo
Domínguez-Figueroa, José
Martínez-Gómez, Ángela
Gómez-Rojas, Almudena
Moreno-Risueño, Miguel Ángel
Fenoll, Carmen
Escobar, Carolina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Junta de Comunidades de Castilla-La Mancha
European Commission
Abril-Urias, Patricia [0000-0003-0677-5600]
Ruiz-Ferrer, Virginia [0000-0002-7840-297X]
Cabrera, Javier [0000-0002-9277-4876]
Olmo, Rocio [0000-0002-4064-9504]
Silva, Ana Cláudia [0000-0002-6113-9722]
Díaz-Manzano, Fernando Evaristo [0000-0002-2064-5303]
Domínguez-Figueroa, José [0000-0002-2614-557X]
Martínez-Gómez, Ángela [0000-0002-8920-4725]
Gómez-Rojas, Almudena [0000-0002-7397-4768]
Moreno-Risueño, Miguel Ángel [0000-0002-9794-1450]
Fenoll, Carmen [0000-0003-4653-6268]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Auxin regulated genes
Galls
Giant cells
Lateral root formation
Syncytia
topic Auxin regulated genes
Galls
Giant cells
Lateral root formation
Syncytia
description 13 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/311758
https://api.elsevier.com/content/abstract/scopus_id/85149493267
url http://hdl.handle.net/10261/311758
https://api.elsevier.com/content/abstract/scopus_id/85149493267
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105924RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111523GB-I00
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://doi.org/10.3389/fpls.2023.1024815
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Divergent regulation of auxin responsive genes in root-knot and cyst nematodes feeding sites formed in ArabidopsisAbril-Urías, PatriciaRuiz-Ferrer, VirginiaCabrera Chaves, JavierOlmo, RocioSilva, Ana CláudiaDíaz-Manzano, Fernando EvaristoDomínguez-Figueroa, JoséMartínez-Gómez, ÁngelaGómez-Rojas, AlmudenaMoreno-Risueño, Miguel ÁngelFenoll, CarmenEscobar, CarolinaAuxin regulated genesGallsGiant cellsLateral root formationSyncytia13 Pág.Cysts (CNs) and root-knot nematodes (RKNs) induce specialized feeding cells, syncytia, and giant cells (GCs), respectively, within plant roots. The plant tissues around the GCs usually by respond forming a root swelling called a gall that contains the GCs. The ontogenesis of feeding cells is different. GC formation is a process of new organogenesis from vascular cells, which are still not well characterized, that differentiate into GCs. In contrast, syncytia formation involves the fusion of adjacent cells that have already differentiated. Nonetheless, both feeding sites show an auxin maximum pertinent to feeding site formation. However, data on the molecular divergences and similarities between the formation of both feeding sites regarding auxin-responsive genes are still scarce. We studied genes from the auxin transduction pathways that are crucial during gall and lateral root (LR) development in the CN interaction by using promoter-reporter (GUS/LUC)transgenic lines, as well as loss of function lines of Arabidopsis. The promoters pGATA23 and several deletions of pmiR390a were active in syncytia, as were in galls, but pAHP6 or putative up-stream regulators as ARF5/7/19 were not active in syncytia. Additionally, none of these genes seemed to play a key role during cyst nematode establishment in Arabidopsis, as the infection rates in loss of function lines did not show significant differences compared to control Col-0 plants. Furthermore, the presence of only canonical AuxRe elements in their proximal promoter regions is highly correlated with their activation in galls/GCs (AHP6, LBD16), but those promoters active in syncytia (miR390, GATA23) carry AuxRe overlapping core cis-elements for other transcription factor families (i.e., bHLH, bZIP). Strikingly, in silico transcriptomic analysis showed very few genes upregulated by auxins common to those induced in GCs and syncytia, despite the high number of upregulated IAA responsive genes in syncytia and galls. The complex regulation of auxin transduction pathways, where different members of the auxin response factor (ARF) family may interact with other factors, and the differences in auxin sensitivity, as indicated by the lower induction of the DR5 sensor in syncytia than galls, among other factors, may explain the divergent regulation of auxin responsive genes in the two types of nematode feeding sites.This work was supported by the Spanish Government (PID2019-105924RB-I00, Ministerio de Ciencia e Innovacion, MCIN/AEI/10.13039/501100011033 and RED2018-102407-T), by the Castilla-La Mancha Government (SBPLY/17/180501/000287; SBPLY/21/180501/000033), o CE; and by MCIN of Spain and ERDF (grant PID2019-111523GB-I00) to MM-R.Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Junta de Comunidades de Castilla-La ManchaEuropean CommissionAbril-Urias, Patricia [0000-0003-0677-5600]Ruiz-Ferrer, Virginia [0000-0002-7840-297X]Cabrera, Javier [0000-0002-9277-4876]Olmo, Rocio [0000-0002-4064-9504]Silva, Ana Cláudia [0000-0002-6113-9722]Díaz-Manzano, Fernando Evaristo [0000-0002-2064-5303]Domínguez-Figueroa, José [0000-0002-2614-557X]Martínez-Gómez, Ángela [0000-0002-8920-4725]Gómez-Rojas, Almudena [0000-0002-7397-4768]Moreno-Risueño, Miguel Ángel [0000-0002-9794-1450]Fenoll, Carmen [0000-0003-4653-6268]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/311758https://api.elsevier.com/content/abstract/scopus_id/85149493267reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105924RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111523GB-I00Centro de Biotecnología y Genómica de Plantas (CBGP)https://doi.org/10.3389/fpls.2023.1024815Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3117582026-05-22T06:33:51Z
score 15,811543

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