Elucidating the role of polygalacturonase genes in strawberry fruit softening
To disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacles, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than those of the wild type when PG genes were silenced individually. Simultaneous silencing...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/373993 |
| Acceso en línea: | http://hdl.handle.net/10261/373993 https://api.elsevier.com/content/abstract/scopus_id/85100156298 |
| Access Level: | acceso abierto |
| Palabra clave: | Fragaria×ananassa Cell wall fruit ripening fruit softening oligosaccharides pectins polygalacturonase |
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Elucidating the role of polygalacturonase genes in strawberry fruit softeningPaniagua, CandelasRic-Varas, PabloGarcía-Gago, Juan ALópez-Casado, GloriaBlanco-Portales, RosarioMuñoz-Blanco, JuanSchückel, JuliaKnox, J PaulMatas, Antonio JQuesada, Miguel APosé, SaraMercado, José AFragaria×ananassaCell wallfruit ripeningfruit softeningoligosaccharidespectinspolygalacturonaseTo disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacles, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than those of the wild type when PG genes were silenced individually. Simultaneous silencing of both PG genes by transgene stacking did not result in an additional increase in firmness. Cell walls from ripe fruits were characterized by a carbohydrate microarray. Higher signals of homogalacturonan and rhamnogalacturonan I pectin epitopes in polysaccharide fractions tightly bound to the cell wall were observed in the transgenic genotypes, suggesting a lower pectin solubilization. At the transcriptomic level, the suppression of FaPG1 or FaPG2 alone induced few transcriptomic changes in the ripe receptacle, but the amount of differentially expressed genes increased notably when both genes were silenced. Many genes encoding cell wall-modifying enzymes were down-regulated. The expression of a putative high affinity potassium transporter was induced in all transgenic genotypes, indicating that cell wall weakening and loss of cell turgor could be linked. These results suggest that, besides the disassembly of pectins tightly linked to the cell wall, PGs could play other roles in strawberry softening, such as the release of oligogalacturonides exerting a positive feedback in softening.This work was supported by the Ministerio de Ciencia, Innovación y Universidades and FEDER EU funds (grant nos AGL2014-55784-C2-1-R and AGL2017-86531-C2-1-R). PR-V was awarded a PhD Fellowship from the Ministerio de Ciencia, Innovación y Universidades (BES-2015–073616) PhD Program Advanced Biotechnology, University of Málaga.Peer reviewedOxford University PressMinisterio de Ciencia, Innovación y Universidades (España)European CommissionUniversidad de MálagaMercado, José A [0000-0003-4351-6261]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/373993https://api.elsevier.com/content/abstract/scopus_id/85100156298reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2014-55784-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-86531-C2-1-Rinfo:eu-repo/grantAgreement///BES-2015–073616Journal of experimental botanyhttps://doi.org/10.1093/jxb/eraa398Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3739932026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| title |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| spellingShingle |
Elucidating the role of polygalacturonase genes in strawberry fruit softening Paniagua, Candelas Fragaria×ananassa Cell wall fruit ripening fruit softening oligosaccharides pectins polygalacturonase |
| title_short |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| title_full |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| title_fullStr |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| title_full_unstemmed |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| title_sort |
Elucidating the role of polygalacturonase genes in strawberry fruit softening |
| dc.creator.none.fl_str_mv |
Paniagua, Candelas Ric-Varas, Pablo García-Gago, Juan A López-Casado, Gloria Blanco-Portales, Rosario Muñoz-Blanco, Juan Schückel, Julia Knox, J Paul Matas, Antonio J Quesada, Miguel A Posé, Sara Mercado, José A |
| author |
Paniagua, Candelas |
| author_facet |
Paniagua, Candelas Ric-Varas, Pablo García-Gago, Juan A López-Casado, Gloria Blanco-Portales, Rosario Muñoz-Blanco, Juan Schückel, Julia Knox, J Paul Matas, Antonio J Quesada, Miguel A Posé, Sara Mercado, José A |
| author_role |
author |
| author2 |
Ric-Varas, Pablo García-Gago, Juan A López-Casado, Gloria Blanco-Portales, Rosario Muñoz-Blanco, Juan Schückel, Julia Knox, J Paul Matas, Antonio J Quesada, Miguel A Posé, Sara Mercado, José A |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Ciencia, Innovación y Universidades (España) European Commission Universidad de Málaga Mercado, José A [0000-0003-4351-6261] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Fragaria×ananassa Cell wall fruit ripening fruit softening oligosaccharides pectins polygalacturonase |
| topic |
Fragaria×ananassa Cell wall fruit ripening fruit softening oligosaccharides pectins polygalacturonase |
| description |
To disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacles, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than those of the wild type when PG genes were silenced individually. Simultaneous silencing of both PG genes by transgene stacking did not result in an additional increase in firmness. Cell walls from ripe fruits were characterized by a carbohydrate microarray. Higher signals of homogalacturonan and rhamnogalacturonan I pectin epitopes in polysaccharide fractions tightly bound to the cell wall were observed in the transgenic genotypes, suggesting a lower pectin solubilization. At the transcriptomic level, the suppression of FaPG1 or FaPG2 alone induced few transcriptomic changes in the ripe receptacle, but the amount of differentially expressed genes increased notably when both genes were silenced. Many genes encoding cell wall-modifying enzymes were down-regulated. The expression of a putative high affinity potassium transporter was induced in all transgenic genotypes, indicating that cell wall weakening and loss of cell turgor could be linked. These results suggest that, besides the disassembly of pectins tightly linked to the cell wall, PGs could play other roles in strawberry softening, such as the release of oligogalacturonides exerting a positive feedback in softening. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
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article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/373993 https://api.elsevier.com/content/abstract/scopus_id/85100156298 |
| url |
http://hdl.handle.net/10261/373993 https://api.elsevier.com/content/abstract/scopus_id/85100156298 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2014-55784-C2-1-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-86531-C2-1-R info:eu-repo/grantAgreement///BES-2015–073616 Journal of experimental botany https://doi.org/10.1093/jxb/eraa398 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Oxford University Press |
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Oxford University Press |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869402754976317440 |
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15.81155 |