Methods to study adult hippocampal neurogenesis in humans and across the phylogeny

The hippocampus hosts the continuous addition of new neurons throughout life—a phenomenon named adult hippocampal neurogenesis (AHN). Here we revisit the occurrence of AHN in more than 110 mammalian species, including humans, and discuss the further validation of these data by single-cell RNAseq and...

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Autores: Terreros Roncal, Julia, Flor García, Miguel de la, Moreno Jiménez, Elena, Rodríguez-Moreno, Carla B., Márquez-Valadez, Berenice, Gallardo-Caballero, Marta, Rábano, Alberto, Llorens Martín, María Victoria
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/707248
Acceso en línea:http://hdl.handle.net/10486/707248
https://dx.doi.org/10.1002/hipo.23474
Access Level:acceso abierto
Palabra clave:Hippocampus
Neural Stem Cells
Neurogenesis
Neurons
Phylogeny
Biología y Biomedicina / Biología
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spelling Methods to study adult hippocampal neurogenesis in humans and across the phylogenyTerreros Roncal, JuliaFlor García, Miguel de laMoreno Jiménez, ElenaRodríguez-Moreno, Carla B.Márquez-Valadez, BereniceGallardo-Caballero, MartaRábano, AlbertoLlorens Martín, María VictoriaHippocampusNeural Stem CellsNeurogenesisNeuronsPhylogenyBiología y Biomedicina / BiologíaThe hippocampus hosts the continuous addition of new neurons throughout life—a phenomenon named adult hippocampal neurogenesis (AHN). Here we revisit the occurrence of AHN in more than 110 mammalian species, including humans, and discuss the further validation of these data by single-cell RNAseq and other alternative techniques. In this regard, our recent studies have addressed the long-standing controversy in the field, namely whether cells positive for AHN markers are present in the adult human dentate gyrus (DG). Here we review how we developed a tightly controlled methodology, based on the use of high-quality brain samples (characterized by short postmortem delays and ≤24 h of fixation in freshly prepared 4% paraformaldehyde), to address human AHN. We review that the detection of AHN markers in samples fixed for 24 h required mild antigen retrieval and chemical elimination of autofluorescence. However, these steps were not necessary for samples subjected to shorter fixation periods. Moreover, the detection of labile epitopes (such as Nestin) in the human hippocampus required the use of mild detergents. The application of this strictly controlled methodology allowed reconstruction of the entire AHN process, thus revealing the presence of neural stem cells, proliferative progenitors, neuroblasts, and immature neurons at distinct stages of differentiation in the human DG. The data reviewed here demonstrate that methodology is of utmost importance when studying AHN by means of distinct techniques across the phylogenetic scale. In this regard, we summarize the major findings made by our group that emphasize that overlooking fundamental technical principles might have consequences for any given research fieldAssociation for Frontotemporal Degeneration; Banco de Santander; Center for Networked Biomedical Research on Neurodegenerative Diseases; Consejo Nacional de Ciencia y Tecnología (CONACYT), Grant/Award Number: 385084; European Research Council, Grant/Award Number: ERC-CoG2020-101001916; Fundación Ramón Areces; Secretaria de Educación, Ciencia Tecnología e Innovación (SECTEI) of the Regional Government of Ciudad de México (CDMX), Grant/Award Number: SECTEI/159/2021; Spanish Ministry of Economy and Competitiveness, Grant/Award Numbers: PID2020-113007RB-I00, RYC-2015-171899WileyDepartamento de Biología MolecularFacultad de Ciencias20232023-04-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/707248https://dx.doi.org/10.1002/hipo.23474reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7072482026-06-23T12:46:27Z
dc.title.none.fl_str_mv Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
title Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
spellingShingle Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
Terreros Roncal, Julia
Hippocampus
Neural Stem Cells
Neurogenesis
Neurons
Phylogeny
Biología y Biomedicina / Biología
title_short Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
title_full Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
title_fullStr Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
title_full_unstemmed Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
title_sort Methods to study adult hippocampal neurogenesis in humans and across the phylogeny
dc.creator.none.fl_str_mv Terreros Roncal, Julia
Flor García, Miguel de la
Moreno Jiménez, Elena
Rodríguez-Moreno, Carla B.
Márquez-Valadez, Berenice
Gallardo-Caballero, Marta
Rábano, Alberto
Llorens Martín, María Victoria
author Terreros Roncal, Julia
author_facet Terreros Roncal, Julia
Flor García, Miguel de la
Moreno Jiménez, Elena
Rodríguez-Moreno, Carla B.
Márquez-Valadez, Berenice
Gallardo-Caballero, Marta
Rábano, Alberto
Llorens Martín, María Victoria
author_role author
author2 Flor García, Miguel de la
Moreno Jiménez, Elena
Rodríguez-Moreno, Carla B.
Márquez-Valadez, Berenice
Gallardo-Caballero, Marta
Rábano, Alberto
Llorens Martín, María Victoria
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Hippocampus
Neural Stem Cells
Neurogenesis
Neurons
Phylogeny
Biología y Biomedicina / Biología
topic Hippocampus
Neural Stem Cells
Neurogenesis
Neurons
Phylogeny
Biología y Biomedicina / Biología
description The hippocampus hosts the continuous addition of new neurons throughout life—a phenomenon named adult hippocampal neurogenesis (AHN). Here we revisit the occurrence of AHN in more than 110 mammalian species, including humans, and discuss the further validation of these data by single-cell RNAseq and other alternative techniques. In this regard, our recent studies have addressed the long-standing controversy in the field, namely whether cells positive for AHN markers are present in the adult human dentate gyrus (DG). Here we review how we developed a tightly controlled methodology, based on the use of high-quality brain samples (characterized by short postmortem delays and ≤24 h of fixation in freshly prepared 4% paraformaldehyde), to address human AHN. We review that the detection of AHN markers in samples fixed for 24 h required mild antigen retrieval and chemical elimination of autofluorescence. However, these steps were not necessary for samples subjected to shorter fixation periods. Moreover, the detection of labile epitopes (such as Nestin) in the human hippocampus required the use of mild detergents. The application of this strictly controlled methodology allowed reconstruction of the entire AHN process, thus revealing the presence of neural stem cells, proliferative progenitors, neuroblasts, and immature neurons at distinct stages of differentiation in the human DG. The data reviewed here demonstrate that methodology is of utmost importance when studying AHN by means of distinct techniques across the phylogenetic scale. In this regard, we summarize the major findings made by our group that emphasize that overlooking fundamental technical principles might have consequences for any given research field
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-04-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/707248
https://dx.doi.org/10.1002/hipo.23474
url http://hdl.handle.net/10486/707248
https://dx.doi.org/10.1002/hipo.23474
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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