Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes
Obese subjects often present a low-grade chronic inflammation in the white adipose tissue, which seems to play an important role in the initiation and development of obesity-related diseases. It has been reported that this inflammatory process may be due to a hypoxic state occuring whithin this tiss...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/37096 |
| Acesso em linha: | https://hdl.handle.net/10171/37096 |
| Access Level: | acceso abierto |
| Palavra-chave: | Obesity Inflammation Hypoxia Hyperoxia Cell culture Adipocytes |
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Influence of different oxygen supply on metabolic markers and gene response in murine adipocytesMartinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Gonzalez-Muniesa, P. (Pedro)|||/items/3c8817f9-abc8-4a50-8bbd-68c967b4df48Quintero, P. (Pablo)|||/items/06c1f69b-2b7b-48f7-b849-8e6ab4667147ObesityInflammationHypoxiaHyperoxiaCell cultureAdipocytesObese subjects often present a low-grade chronic inflammation in the white adipose tissue, which seems to play an important role in the initiation and development of obesity-related diseases. It has been reported that this inflammatory process may be due to a hypoxic state occuring whithin this tissue. Oxygen is used in current medicine as a treatment for several conditions. The aim of this study was to analyze the effects of 95% O2 on specific metabolic variables and on the expression of some adipokines on murine adipocytes. 3T3-L1 adipocytes were exposed during 48 h to different treatments: 95% O2 hyperoxia (HPx group), CoCl2 (CoCl2 group), hyperoxia with CoCl2 (HPx+CoCl2 group) and 1%O2 hypoxia (Hx group). Cell viability, intracellular ROS content, glucose utilization, lactate and glycerol concentrations were measured. Also, mRNA expression of HIF-1[alfa], GLUT-1, ANGPTL4, PPAR-[gamma], adiponectin, IL-6 and MCP-1 genes was analyzed. Importantly, 95% O2 decreased cell viability and increased intracellular ROS production. Also, glycerol and lactate release were significantly increased and decreased, respectively, in HPx treated cells. This treatment also provoked a downregulation of GLUT-1 and ANGPTL-4, while IL-6 and MCP-1 were up-regulated. Exposure to a hyperoxia of 95% O2 seemed to provoke an inflammatory response in adipocytes. The two hypoxia-inducing conditions (CoCl2 and 1% O2) produced different outcomes in metabolic measurements as well as in the expression of some genes (GLUT-1, ANPGTL4, PPAR-[gamma] and adiponectin), while it remained similar in others (HIF-1[alfa], IL-6 and MCP-1). Indeed, hyperoxia increased significantly the ROS levels and the lipolytic activity, while it reduced lactate production. In addition to the effects on inflammation, the changes in GLUT-1, ANGPTL4 and PPAR-[gamma] genes let suppose that hyperoxia may be beneficial for the hypertrophied adipose tissues of obese subjects and for improving insulin sensitivity.Wichtig EditoreDadun. Depósito Académico Digital Universidad de Navarra20142014-11-2420122012-01-0120122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfimage/jpegimage/jpegimage/jpegimage/jpegimage/jpeghttps://hdl.handle.net/10171/37096reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/370962026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| title |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| spellingShingle |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 Obesity Inflammation Hypoxia Hyperoxia Cell culture Adipocytes |
| title_short |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| title_full |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| title_fullStr |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| title_full_unstemmed |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| title_sort |
Influence of different oxygen supply on metabolic markers and gene response in murine adipocytes |
| dc.creator.none.fl_str_mv |
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 Gonzalez-Muniesa, P. (Pedro)|||/items/3c8817f9-abc8-4a50-8bbd-68c967b4df48 Quintero, P. (Pablo)|||/items/06c1f69b-2b7b-48f7-b849-8e6ab4667147 |
| author |
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 |
| author_facet |
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 Gonzalez-Muniesa, P. (Pedro)|||/items/3c8817f9-abc8-4a50-8bbd-68c967b4df48 Quintero, P. (Pablo)|||/items/06c1f69b-2b7b-48f7-b849-8e6ab4667147 |
| author_role |
author |
| author2 |
Gonzalez-Muniesa, P. (Pedro)|||/items/3c8817f9-abc8-4a50-8bbd-68c967b4df48 Quintero, P. (Pablo)|||/items/06c1f69b-2b7b-48f7-b849-8e6ab4667147 |
| author2_role |
author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Obesity Inflammation Hypoxia Hyperoxia Cell culture Adipocytes |
| topic |
Obesity Inflammation Hypoxia Hyperoxia Cell culture Adipocytes |
| description |
Obese subjects often present a low-grade chronic inflammation in the white adipose tissue, which seems to play an important role in the initiation and development of obesity-related diseases. It has been reported that this inflammatory process may be due to a hypoxic state occuring whithin this tissue. Oxygen is used in current medicine as a treatment for several conditions. The aim of this study was to analyze the effects of 95% O2 on specific metabolic variables and on the expression of some adipokines on murine adipocytes. 3T3-L1 adipocytes were exposed during 48 h to different treatments: 95% O2 hyperoxia (HPx group), CoCl2 (CoCl2 group), hyperoxia with CoCl2 (HPx+CoCl2 group) and 1%O2 hypoxia (Hx group). Cell viability, intracellular ROS content, glucose utilization, lactate and glycerol concentrations were measured. Also, mRNA expression of HIF-1[alfa], GLUT-1, ANGPTL4, PPAR-[gamma], adiponectin, IL-6 and MCP-1 genes was analyzed. Importantly, 95% O2 decreased cell viability and increased intracellular ROS production. Also, glycerol and lactate release were significantly increased and decreased, respectively, in HPx treated cells. This treatment also provoked a downregulation of GLUT-1 and ANGPTL-4, while IL-6 and MCP-1 were up-regulated. Exposure to a hyperoxia of 95% O2 seemed to provoke an inflammatory response in adipocytes. The two hypoxia-inducing conditions (CoCl2 and 1% O2) produced different outcomes in metabolic measurements as well as in the expression of some genes (GLUT-1, ANPGTL4, PPAR-[gamma] and adiponectin), while it remained similar in others (HIF-1[alfa], IL-6 and MCP-1). Indeed, hyperoxia increased significantly the ROS levels and the lipolytic activity, while it reduced lactate production. In addition to the effects on inflammation, the changes in GLUT-1, ANGPTL4 and PPAR-[gamma] genes let suppose that hyperoxia may be beneficial for the hypertrophied adipose tissues of obese subjects and for improving insulin sensitivity. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2012-01-01 2012 2012-01-01 2014 2014-11-24 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/37096 |
| url |
https://hdl.handle.net/10171/37096 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf image/jpeg image/jpeg image/jpeg image/jpeg image/jpeg |
| dc.publisher.none.fl_str_mv |
Wichtig Editore |
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Wichtig Editore |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15,301603 |