Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application

Cèl·lules estromals mesenquimals; Gelatina de Wharton; Diferenciació osteogènica

Detalhes bibliográficos
Autores: Cabrera Perez, Raquel, Gámez-Valero, Ana, Rojas-Márquez, Raquel, Borràs, Francesc Enric, Roura, Santiago, Vives Armengol, Joaquim, Monguió-Tortajada, Marta
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/5856
Acesso em linha:https://hdl.handle.net/11351/5856
Access Level:acceso abierto
Palavra-chave:Cèl·lules de la medul·la òssia
Ossos - Creixement
ANATOMY::Cells::Bone Marrow Cells
PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Development::Biomineralization::Bone Development::Osteogenesis
ANATOMÍA::células::células de la médula ósea
FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::desarrollo musculoesquelético::biomineralización::desarrollo óseo::osteogénesis
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
title Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
spellingShingle Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
Cabrera Perez, Raquel
Cèl·lules de la medul·la òssia
Ossos - Creixement
ANATOMY::Cells::Bone Marrow Cells
PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Development::Biomineralization::Bone Development::Osteogenesis
ANATOMÍA::células::células de la médula ósea
FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::desarrollo musculoesquelético::biomineralización::desarrollo óseo::osteogénesis
title_short Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
title_full Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
title_fullStr Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
title_full_unstemmed Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
title_sort Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
dc.creator.none.fl_str_mv Cabrera Perez, Raquel
Gámez-Valero, Ana
Rojas-Márquez, Raquel
Borràs, Francesc Enric
Roura, Santiago
Vives Armengol, Joaquim
Monguió-Tortajada, Marta
author Cabrera Perez, Raquel
author_facet Cabrera Perez, Raquel
Gámez-Valero, Ana
Rojas-Márquez, Raquel
Borràs, Francesc Enric
Roura, Santiago
Vives Armengol, Joaquim
Monguió-Tortajada, Marta
author_role author
author2 Gámez-Valero, Ana
Rojas-Márquez, Raquel
Borràs, Francesc Enric
Roura, Santiago
Vives Armengol, Joaquim
Monguió-Tortajada, Marta
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Cabrera-Pérez R] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Grup Enginyeria Tissular Musculoesquelètica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Monguió-Tortajada M, Gámez-Valero A] REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. [Rojas-Márquez R] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation (CRG), Barcelona, Catalonia, Spain. [Borràs FE] REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. Nephrology Service, Germans Trias i Pujol University Hospital, Badalona, Catalonia, Spain. [Roura S] ICREC Research Program, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. [Vives J] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Grup Enginyeria Tissular Musculoesquelètica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Cèl·lules de la medul·la òssia
Ossos - Creixement
ANATOMY::Cells::Bone Marrow Cells
PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Development::Biomineralization::Bone Development::Osteogenesis
ANATOMÍA::células::células de la médula ósea
FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::desarrollo musculoesquelético::biomineralización::desarrollo óseo::osteogénesis
topic Cèl·lules de la medul·la òssia
Ossos - Creixement
ANATOMY::Cells::Bone Marrow Cells
PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Development::Biomineralization::Bone Development::Osteogenesis
ANATOMÍA::células::células de la médula ósea
FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::desarrollo musculoesquelético::biomineralización::desarrollo óseo::osteogénesis
description Cèl·lules estromals mesenquimals; Gelatina de Wharton; Diferenciació osteogènica
publishDate 2019
dc.date.none.fl_str_mv 2019
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/5856
url https://hdl.handle.net/11351/5856
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Stem Cell Research & Therapy;10(1)
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1450-3
info:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F01788
info:eu-repo/grantAgreement/ES/PE2013-2016/RD16%2F0009
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402735103705088
spelling Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical applicationCabrera Perez, RaquelGámez-Valero, AnaRojas-Márquez, RaquelBorràs, Francesc EnricRoura, SantiagoVives Armengol, JoaquimMonguió-Tortajada, MartaCèl·lules de la medul·la òssiaOssos - CreixementANATOMY::Cells::Bone Marrow CellsPHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Development::Biomineralization::Bone Development::OsteogenesisANATOMÍA::células::células de la médula óseaFENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::desarrollo musculoesquelético::biomineralización::desarrollo óseo::osteogénesisCèl·lules estromals mesenquimals; Gelatina de Wharton; Diferenciació osteogènicaCélulas estromales mesenquimales; Gelatina de Wharton; Diferenciación osteogénicaMesenchymal stromal cells, Wharton’s jelly; Osteogenic differentiationBackground Orthopaedic diseases are one of the major targets for regenerative medicine. In this context, Wharton’s jelly (WJ) is an alternative source to bone marrow (BM) for allogeneic transplantation since its isolation does not require an invasive procedure for cell collection and does not raise major ethical concerns. However, the osteogenic capacity of human WJ-derived multipotent mesenchymal stromal cells (MSC) remains unclear. Methods Here, we compared the baseline osteogenic potential of MSC from WJ and BM cell sources by cytological staining, quantitative real-time PCR and proteomic analysis, and assessed chemical and biological strategies for priming undifferentiated WJ-MSC. Concretely, different inhibitors/activators of the TGFβ1-BMP2 signalling pathway as well as the secretome of differentiating BM-MSC were tested. Results Cytochemical staining as well as gene expression and proteomic analysis revealed that osteogenic commitment was poor in WJ-MSC. However, stimulation of the BMP2 pathway with BMP2 plus tanshinone IIA and the addition of extracellular vesicles or protein-enriched preparations from differentiating BM-MSC enhanced WJ-MSC osteogenesis. Furthermore, greater outcome was obtained with the use of conditioned media from differentiating BM-MSC. Conclusions Altogether, our results point to the use of master banks of WJ-MSC as a valuable alternative to BM-MSC for orthopaedic conditions.Work in our laboratory is supported by the Spanish Cell Therapy Network (TerCel, expedient No. RD16/0011/0028 and RD16/00111/0006), Ministerio de Ciencia Innovación y Universidades de España (Instituto de Salud Carlos III, expedient No. PI19/01788), Fundació la Marató de TV3 (expedient number: 122831), and ISCIII-REDinREN (RD16/0009 Feder Funds), and developed in the context of AdvanceCat with the support of ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya) under the Catalonian ERDF operational programme (European Regional Development Fund) 2014-2020. FEB is a researcher from Fundació Institut de Recerca en Ciències de la Salut Germans Trias i Pujol, supported by the Health Department of the Catalan Government (Generalitat de Catalunya). Our laboratory is awarded by the Generalitat de Catalunya as Consolidated Research Groups (ref. 2017-SGR-719, 2017-SGR-301 and 2017-SGR-483).BMCInstitut Català de la Salut[Cabrera-Pérez R] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Grup Enginyeria Tissular Musculoesquelètica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Monguió-Tortajada M, Gámez-Valero A] REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. [Rojas-Márquez R] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation (CRG), Barcelona, Catalonia, Spain. [Borràs FE] REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. Nephrology Service, Germans Trias i Pujol University Hospital, Badalona, Catalonia, Spain. [Roura S] ICREC Research Program, Health Science Research Institute Germans Trias i Pujol (IGTP), Badalona, Catalonia, Spain. [Vives J] Cell Therapy Service, Blood and Tissue Bank (BST), Barcelona, Catalonia, Spain. Grup Enginyeria Tissular Musculoesquelètica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona (UAB), Bellaterra, SpainVall d'Hebron Barcelona Hospital Campus202120212019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/5856Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésStem Cell Research & Therapy;10(1)https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1450-3info:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F01788info:eu-repo/grantAgreement/ES/PE2013-2016/RD16%2F0009Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/58562026-06-12T09:38:37Z
score 15,811543

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