Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines

Producción Científica

Bibliographic Details
Authors: Torres Franco, Andrés Felipe, Herrero Lobo, Raquel, Lebrero Fernández, Raquel, Muñoz Torre, Raúl
Format: article
Status:Published version
Publication Date:2026
Country:España
Institution:Universidad de Valladolid
Repository:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/81668
Online Access:https://doi.org/10.1016/j.biortech.2026.133920
https://uvadoc.uva.es/handle/10324/81668
Access Level:Open access
Keyword:Ectoine biosynthesis
Ectoine extraction
Methane bioconversion
Taylor Flow reactor
3308 Ingeniería y Tecnología del Medio Ambiente
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spelling Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoinesTorres Franco, Andrés FelipeHerrero Lobo, RaquelLebrero Fernández, RaquelMuñoz Torre, RaúlEctoine biosynthesisEctoine extractionMethane bioconversionTaylor Flow reactor3308 Ingeniería y Tecnología del Medio AmbienteProducción CientíficaEctoine is one of the most profitable value chains for biogas valorisation. This study assessed the long-term effects of biomilking for ectoine (Ect) and hydroxyectoine (Hy) extraction on upstream methane bioconversion into ectoines using a halotolerant methanotrophic consortium cultivated in a Taylor-Flow bioreactor. After a control stage (S-I), fractions of the culture broth volume of 10 %, 50 %, 30 % and 60 % (S-II to S-VI) were subjected to biomilking before a final control (S-VII). No adverse effects were observed at 10 %, while higher fractions led to salt depletion, a ~10 % reduction in CH4 bioconversion efficiency, and a loss of dominant ectoine producers, mainly Methylomicrobium. A decrease in intracellular Ect was also observed. Restoring salt levels (S- VI) recovered Methylomicrobium dominance and Ect content in the culture broth. Enhanced biomilking yielded up to 27.2 g-Ect + Hy per inlet kg of CH4, supporting its feasibility for sustainable biogas valorisation at a com- mercial scale.Esta investigación fue financiada por el proyecto Horizonte CHEERS de la UE, financiado por el programa de Investigación e Innovación Horizonte Europa de la Unión Europea bajo el Acuerdo de Subvención No 101060814Proyecto Horizonte Europa CHEERS cuentan con el apoyo de las subvenciones UKRI número 10050977 Earthwatch EuropeJunta de Castilla y León (Consejería de Educación) y la Unión Europea a través del Fondo Europeo de Desarrollo Regional (FEDER) (Referencia: CLU-2025-2-06)Elsevier2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.biortech.2026.133920https://uvadoc.uva.es/handle/10324/81668reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0960852426000015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/816682026-06-13T12:44:47Z
dc.title.none.fl_str_mv Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
title Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
spellingShingle Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
Torres Franco, Andrés Felipe
Ectoine biosynthesis
Ectoine extraction
Methane bioconversion
Taylor Flow reactor
3308 Ingeniería y Tecnología del Medio Ambiente
title_short Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
title_full Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
title_fullStr Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
title_full_unstemmed Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
title_sort Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
dc.creator.none.fl_str_mv Torres Franco, Andrés Felipe
Herrero Lobo, Raquel
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
author Torres Franco, Andrés Felipe
author_facet Torres Franco, Andrés Felipe
Herrero Lobo, Raquel
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
author_role author
author2 Herrero Lobo, Raquel
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
author2_role author
author
author
dc.subject.none.fl_str_mv Ectoine biosynthesis
Ectoine extraction
Methane bioconversion
Taylor Flow reactor
3308 Ingeniería y Tecnología del Medio Ambiente
topic Ectoine biosynthesis
Ectoine extraction
Methane bioconversion
Taylor Flow reactor
3308 Ingeniería y Tecnología del Medio Ambiente
description Producción Científica
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.biortech.2026.133920
https://uvadoc.uva.es/handle/10324/81668
url https://doi.org/10.1016/j.biortech.2026.133920
https://uvadoc.uva.es/handle/10324/81668
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0960852426000015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402702616723456
score 15,811543

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