Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1

During the development of the central nervous system (CNS), only motor axons project into peripheral nerves. Little is known about the cellular and molecular mechanisms that control the development of a boundary at the CNS surface and prevent CNS neuron emigration from the neural tube. It has previo...

ver descrição completa

Detalhes bibliográficos
Autores: Moreno-Bravo, Juan Antonio, Roig-Puiggros, Sergi, Blockus, Heike, Dominici, Chloé, Zelina, Pavol, Mehlen, Patrick, Chédotal, Alain
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/289409
Acesso em linha:http://hdl.handle.net/10261/289409
Access Level:acceso abierto
Palavra-chave:Netrin
Dcc
Pontine neurons
Cerebellum
Migration
Commissural neurons
id ES_02f911db3de561ae56da78c358dc2e4a
oai_identifier_str oai:digital.csic.es:10261/289409
network_acronym_str ES
network_name_str España
repository_id_str
spelling Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1Moreno-Bravo, Juan AntonioRoig-Puiggros, SergiBlockus, HeikeDominici, ChloéZelina, PavolMehlen, PatrickChédotal, AlainNetrinDccPontine neuronsCerebellumMigrationCommissural neuronsDuring the development of the central nervous system (CNS), only motor axons project into peripheral nerves. Little is known about the cellular and molecular mechanisms that control the development of a boundary at the CNS surface and prevent CNS neuron emigration from the neural tube. It has previously been shown that a subset of spinal cord commissural axons abnormally invades sensory nerves in Ntn1 hypomorphic embryos and Dcc knockouts. However, whether netrin 1 also plays a similar role in the brain is unknown. In the hindbrain, precerebellar neurons migrate tangentially under the pial surface, and their ventral migration is guided by netrin 1. Here, we show that pontine neurons and inferior olivary neurons, two types of precerebellar neurons, are not confined to the CNS in Ntn1 and Dcc mutant mice, but that they invade the trigeminal, auditory and vagus nerves. Using a Ntn1 conditional knockout, we show that netrin 1, which is released at the pial surface by ventricular zone progenitors is responsible for the CNS confinement of precerebellar neurons. We propose, that netrin 1 distribution sculpts the CNS boundary by keeping CNS neurons in netrin 1-rich domains.This work was supported by grants from the Agence Nationale de la Recherche (ANR-14-CE13-0004-01) (to A.C.). It was performed in the frame of the Labex Lifesenses (ANR-10-LABX-65) supported by French state funds managed by the Agence Nationale de la Recherche within the Investissements d'Avenir programme under ANR-11-IDEX-0004-02 (to A.C.).Peer reviewedCompany of BiologistsAgence Nationale de la Recherche (France)202320232018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/289409reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDevelopmenthttps://doi.org/10.1242/dev.159400Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2894092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
title Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
spellingShingle Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
Moreno-Bravo, Juan Antonio
Netrin
Dcc
Pontine neurons
Cerebellum
Migration
Commissural neurons
title_short Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
title_full Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
title_fullStr Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
title_full_unstemmed Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
title_sort Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
dc.creator.none.fl_str_mv Moreno-Bravo, Juan Antonio
Roig-Puiggros, Sergi
Blockus, Heike
Dominici, Chloé
Zelina, Pavol
Mehlen, Patrick
Chédotal, Alain
author Moreno-Bravo, Juan Antonio
author_facet Moreno-Bravo, Juan Antonio
Roig-Puiggros, Sergi
Blockus, Heike
Dominici, Chloé
Zelina, Pavol
Mehlen, Patrick
Chédotal, Alain
author_role author
author2 Roig-Puiggros, Sergi
Blockus, Heike
Dominici, Chloé
Zelina, Pavol
Mehlen, Patrick
Chédotal, Alain
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agence Nationale de la Recherche (France)
dc.subject.none.fl_str_mv Netrin
Dcc
Pontine neurons
Cerebellum
Migration
Commissural neurons
topic Netrin
Dcc
Pontine neurons
Cerebellum
Migration
Commissural neurons
description During the development of the central nervous system (CNS), only motor axons project into peripheral nerves. Little is known about the cellular and molecular mechanisms that control the development of a boundary at the CNS surface and prevent CNS neuron emigration from the neural tube. It has previously been shown that a subset of spinal cord commissural axons abnormally invades sensory nerves in Ntn1 hypomorphic embryos and Dcc knockouts. However, whether netrin 1 also plays a similar role in the brain is unknown. In the hindbrain, precerebellar neurons migrate tangentially under the pial surface, and their ventral migration is guided by netrin 1. Here, we show that pontine neurons and inferior olivary neurons, two types of precerebellar neurons, are not confined to the CNS in Ntn1 and Dcc mutant mice, but that they invade the trigeminal, auditory and vagus nerves. Using a Ntn1 conditional knockout, we show that netrin 1, which is released at the pial surface by ventricular zone progenitors is responsible for the CNS confinement of precerebellar neurons. We propose, that netrin 1 distribution sculpts the CNS boundary by keeping CNS neurons in netrin 1-rich domains.
publishDate 2018
dc.date.none.fl_str_mv 2018
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/289409
url http://hdl.handle.net/10261/289409
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Development
https://doi.org/10.1242/dev.159400
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402693943951360
score 15,811543

Registos relacionados