The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction

Ochratoxin A (OTA), a nephrotoxic compound produced by certain Aspergillus and Penicillium species, is one of the most abundant mycotoxins in food commodities. Aspergillus carbonarius is the main source of OTA in wine, grape juice and dried vine fruits. Although many studies have focused on OTA prod...

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Authors: Crespo Sempere, Ana, Martínez-Culebras, Pedro V., González-Candelas, Luis
Format: article
Status:Versión aceptada para publicación
Publication Date:2014
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127723
Online Access:http://hdl.handle.net/10261/127723
Access Level:Open access
Keyword:Ochratoxin production
Aspergillus carbonarius
Major facilitator superfamily transporters
mfsA
Grapes
Wine
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network_acronym_str ES
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repository_id_str
spelling The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproductionCrespo Sempere, AnaMartínez-Culebras, Pedro V.González-Candelas, LuisOchratoxin productionAspergillus carbonariusMajor facilitator superfamily transportersmfsAGrapesWineOchratoxin A (OTA), a nephrotoxic compound produced by certain Aspergillus and Penicillium species, is one of the most abundant mycotoxins in food commodities. Aspergillus carbonarius is the main source of OTA in wine, grape juice and dried vine fruits. Although many studies have focused on OTA production by A. carbonarius, little is known about the genes related to OTA production and transport. We have found a transporter that belongs to the major facilitator superfamily (MfsA) which is highly expressed with a 102-fold induction in an ochratoxigenic A. carbonarius strain compared to a low OTA producer strain. The encoding mfsA gene shows similarity to the multidrug efflux transporter flu1 from Candida albicans. A high number of putative transcription factor binding sites involved in the response to stress were identified within the promoter of mfsA. Phenotypical analysis of δ. mfsA deletion mutants revealed that the loss of mfsA leads to a slight growth reduction and increased OTA production. We therefore hypothesize that MfsA could be a stress response transporter whose disruption could cause an increase in oxidative stress together with a stimulation of mycotoxin production. •A. carbonarius mfsA expression is induced over 100 fold in an OTA producing strain.•mfsA gene has been cloned and sequenced.•An A. carbonarius δmfsA null mutant has been obtained by ATMT.•The δmfsA null mutants produce more OTA than the parental strain.•The expression of mfsA is up regulated by hydrogen peroxide.A. Crespo-Sempere was recipient of a FPI fellowship from the Spanish Government. This research was supported by grants AGL2005-00707 and AGL2008-04828-C03-02 from the Spanish Government.Peer ReviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620142016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127723reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1277232026-05-22T06:33:51Z
dc.title.none.fl_str_mv The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
title The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
spellingShingle The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
Crespo Sempere, Ana
Ochratoxin production
Aspergillus carbonarius
Major facilitator superfamily transporters
mfsA
Grapes
Wine
title_short The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
title_full The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
title_fullStr The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
title_full_unstemmed The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
title_sort The loss of the inducible Aspergillus carbonarius MFS transporter MfsA leads to ochratoxin A overproduction
dc.creator.none.fl_str_mv Crespo Sempere, Ana
Martínez-Culebras, Pedro V.
González-Candelas, Luis
author Crespo Sempere, Ana
author_facet Crespo Sempere, Ana
Martínez-Culebras, Pedro V.
González-Candelas, Luis
author_role author
author2 Martínez-Culebras, Pedro V.
González-Candelas, Luis
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ochratoxin production
Aspergillus carbonarius
Major facilitator superfamily transporters
mfsA
Grapes
Wine
topic Ochratoxin production
Aspergillus carbonarius
Major facilitator superfamily transporters
mfsA
Grapes
Wine
description Ochratoxin A (OTA), a nephrotoxic compound produced by certain Aspergillus and Penicillium species, is one of the most abundant mycotoxins in food commodities. Aspergillus carbonarius is the main source of OTA in wine, grape juice and dried vine fruits. Although many studies have focused on OTA production by A. carbonarius, little is known about the genes related to OTA production and transport. We have found a transporter that belongs to the major facilitator superfamily (MfsA) which is highly expressed with a 102-fold induction in an ochratoxigenic A. carbonarius strain compared to a low OTA producer strain. The encoding mfsA gene shows similarity to the multidrug efflux transporter flu1 from Candida albicans. A high number of putative transcription factor binding sites involved in the response to stress were identified within the promoter of mfsA. Phenotypical analysis of δ. mfsA deletion mutants revealed that the loss of mfsA leads to a slight growth reduction and increased OTA production. We therefore hypothesize that MfsA could be a stress response transporter whose disruption could cause an increase in oxidative stress together with a stimulation of mycotoxin production. •A. carbonarius mfsA expression is induced over 100 fold in an OTA producing strain.•mfsA gene has been cloned and sequenced.•An A. carbonarius δmfsA null mutant has been obtained by ATMT.•The δmfsA null mutants produce more OTA than the parental strain.•The expression of mfsA is up regulated by hydrogen peroxide.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127723
url http://hdl.handle.net/10261/127723
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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