Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

Xenopus tadpoles offer a unique platform to investigate the function of the nervous system. They provide multiple experimental advantages, such as accessibility to numerous imaging approaches, electrophysiological techniques and behavioral assays. The Xenopus tadpole olfactory system is particularly...

ver descrição completa

Detalhes bibliográficos
Autores: Terni, Beatrice, Pacciolla, Paolo, Perelló, Margalida, Llobet Berenguer, Artur, 1972-
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/213382
Acesso em linha:https://hdl.handle.net/2445/213382
Access Level:acceso abierto
Palavra-chave:Animals
Expressió gènica
Larves
Sistema nerviós
Gene expression
Larvae
Nervous system
id ES_02e4f0a8d9a06f21b044fc1de1defcee
oai_identifier_str oai:recercat.cat:2445/213382
network_acronym_str ES
network_name_str España
repository_id_str
spelling Functional Evaluation of Olfactory Pathways in Living Xenopus TadpolesTerni, BeatricePacciolla, PaoloPerelló, MargalidaLlobet Berenguer, Artur, 1972-AnimalsExpressió gènicaLarvesSistema nerviósAnimalsGene expressionLarvaeNervous systemXenopus tadpoles offer a unique platform to investigate the function of the nervous system. They provide multiple experimental advantages, such as accessibility to numerous imaging approaches, electrophysiological techniques and behavioral assays. The Xenopus tadpole olfactory system is particularly well suited to investigate the function of synapses established during normal development or reformed after injury. Here, we describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae. We outline a combination of in vivo measurements of presynaptic calcium responses in glomeruli of the olfactory bulb with olfactory-guided behavior assays. Methods can be combined with the transection of olfactory nerves to study the rewiring of synaptic connectivity. Experiments are presented using both wild-type and genetically modified animals expressing GFP reporters in central nervous system cells. Application of the approaches described to genetically modified tadpoles can be useful for unraveling the molecular bases that define vertebrate behavior.JoVE2024202420182024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfhttps://hdl.handle.net/2445/213382Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3791/58028JoVE. Journal of Visualized Experiments, 2018, num.142https://doi.org/10.3791/58028(c) JoVE, 2018info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2133822026-05-29T05:05:01Z
dc.title.none.fl_str_mv Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
title Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
spellingShingle Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
Terni, Beatrice
Animals
Expressió gènica
Larves
Sistema nerviós
Animals
Gene expression
Larvae
Nervous system
title_short Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
title_full Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
title_fullStr Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
title_full_unstemmed Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
title_sort Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles
dc.creator.none.fl_str_mv Terni, Beatrice
Pacciolla, Paolo
Perelló, Margalida
Llobet Berenguer, Artur, 1972-
author Terni, Beatrice
author_facet Terni, Beatrice
Pacciolla, Paolo
Perelló, Margalida
Llobet Berenguer, Artur, 1972-
author_role author
author2 Pacciolla, Paolo
Perelló, Margalida
Llobet Berenguer, Artur, 1972-
author2_role author
author
author
dc.subject.none.fl_str_mv Animals
Expressió gènica
Larves
Sistema nerviós
Animals
Gene expression
Larvae
Nervous system
topic Animals
Expressió gènica
Larves
Sistema nerviós
Animals
Gene expression
Larvae
Nervous system
description Xenopus tadpoles offer a unique platform to investigate the function of the nervous system. They provide multiple experimental advantages, such as accessibility to numerous imaging approaches, electrophysiological techniques and behavioral assays. The Xenopus tadpole olfactory system is particularly well suited to investigate the function of synapses established during normal development or reformed after injury. Here, we describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae. We outline a combination of in vivo measurements of presynaptic calcium responses in glomeruli of the olfactory bulb with olfactory-guided behavior assays. Methods can be combined with the transection of olfactory nerves to study the rewiring of synaptic connectivity. Experiments are presented using both wild-type and genetically modified animals expressing GFP reporters in central nervous system cells. Application of the approaches described to genetically modified tadpoles can be useful for unraveling the molecular bases that define vertebrate behavior.
publishDate 2018
dc.date.none.fl_str_mv 2018
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/213382
url https://hdl.handle.net/2445/213382
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3791/58028
JoVE. Journal of Visualized Experiments, 2018, num.142
https://doi.org/10.3791/58028
dc.rights.none.fl_str_mv (c) JoVE, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) JoVE, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
dc.publisher.none.fl_str_mv JoVE
publisher.none.fl_str_mv JoVE
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402688337215488
score 15,812429

Registos relacionados