Role of calcium signals on hydrogen peroxide-induced apoptosis in human myeloid HL-60 cells

The present study is aimed to determine the role of calcium signaling evoked by the oxygen radical, hydrogen peroxide (H2O2) and the specific inhibitor of calcium reuptake thapsigargin on apoptosis in the human leukemia cell line HL-60. Our results show that treatment of HL-60 cells with 100 µM H2O2...

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Detalles Bibliográficos
Autores: Bejarano Hernando, Ignacio, Espino Palma, Javier, González Flores, David, García Casado, Javier, Redondo Liberal, Pedro Cosme, Rosado Dionisio, Juan Antonio, Barriga Ibars, M.Carmen, Pariente Llanos, José Antonio, Rodríguez Moratinos, Ana Beatriz
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/38793
Acceso en línea:https://hdl.handle.net/10902/38793
Access Level:acceso abierto
Palabra clave:Caspases
Calcium signal
Apoptosis
Mitochondria
H2O2
HL-60 cells
Descripción
Sumario:The present study is aimed to determine the role of calcium signaling evoked by the oxygen radical, hydrogen peroxide (H2O2) and the specific inhibitor of calcium reuptake thapsigargin on apoptosis in the human leukemia cell line HL-60. Our results show that treatment of HL-60 cells with 100 µM H2O2 and 1 µM thapsigargin induced a transient increase in cytosolic free calcium concentration ([Ca2+]c) due to calcium release from internal stores. These stimulatory effects on calcium signals were followed by activation of the mitocondrial permeability transition pore (mPTP), as well as a time-dependent increase in caspase-9 and -3 activities. Our results also show that H2O2 and thapsigargin were able to increase the relative content of fragmented DNA and phosphatidylserine externalization, as detected by double-staining with propidium iodide (PI) and annexin-V-FITC , respectively. Treatment of cells with H2O2 or thapsigargin resulted in activation of the proapoptotic protein Bid. Furthermore, coimmunoprecipitation experiments showed active Bax was bound to Bid, which regulates Bid activity and promotes apoptosis. Our findings suggest that H2O2- and thapsigargin-induced apoptosis is dependent on rises in [Ca2+]c in human myeloid HL-60 cells.

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