Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems

We propose the experimental use of resazurin (Raz) and develop a metabolically active transient storage (MATS) model to include processes that may provide additional information on transient storage from a biogeochemical perspective in stream ecosystems. Raz is a phenoxazine compound that reduces ir...

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Authors: Haggerty, Roy, Martí Roca, Eugènia, Argerich, Alba, Schiller Calle, Daniel von, Grimm, Nancy B.
Format: article
Status:Published version
Publication Date:2009
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/173291
Online Access:https://hdl.handle.net/2445/173291
Access Level:Open access
Keyword:Biogeoquímica
Ecologia fluvial
Cursos d'aigua
Biogeochemistry
Stream ecology
Rivers
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spelling Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystemsHaggerty, RoyMartí Roca, EugèniaArgerich, AlbaSchiller Calle, Daniel vonGrimm, Nancy B.BiogeoquímicaEcologia fluvialCursos d'aiguaBiogeochemistryStream ecologyRiversWe propose the experimental use of resazurin (Raz) and develop a metabolically active transient storage (MATS) model to include processes that may provide additional information on transient storage from a biogeochemical perspective in stream ecosystems. Raz is a phenoxazine compound that reduces irreversibly to resorufin (Rru) in the presence of aerobic bacteria. Raz was added as a stream tracer to a 128‐m reach of the forested second‐order Riera de Santa Fe del Montseny (Catalonia, NE Spain), along with a conservative tracer, NaCl. Raz was transformed to Rru at a rate of 0.81 h−1 in the hyporheic zone and only at a rate of 9.9 × 10−4 h−1 in the stream surface channel. Raz transformation and decay and Rru production and decay were both correlated with O2 consumption measured at wells. The ratio of Raz to Rru concentration at the bottom of the reach was moderately correlated with instantaneous rates of net ecosystem production (NEP) measured over the whole reach. Data for Raz, Rru, and chloride were well fitted with the MATS model. The results from this study suggest that Raz transformation to Rru can be used as a 'smart' tracer to detect metabolic activity, specifically aerobic respiration, associated with transient storage zones in stream ecosystems. Therefore, the Raz‐Rru system can provide an assessment of the amount of transient storage that is metabolically active, an assessment that complements the physical characterization of transient storage obtained from conventional hydrologic tracers. The use of both physical and metabolic parameters of transient storage obtained with these tracers may increase our understanding of the relevance of transient storage on stream biogeochemical processes at whole reach scale, as well as the contribution of the different transient storage compartments to these processes.Wiley2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/173291Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1029/2008JG000942Journal of Geophysical Research, 2009, vol. 114, num. G03014, p. 1-14https://doi.org/10.1029/2008JG000942(c) American Geophysical Union (AGU) , 2009info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1732912026-05-27T06:46:51Z
dc.title.none.fl_str_mv Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
title Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
spellingShingle Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
Haggerty, Roy
Biogeoquímica
Ecologia fluvial
Cursos d'aigua
Biogeochemistry
Stream ecology
Rivers
title_short Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
title_full Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
title_fullStr Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
title_full_unstemmed Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
title_sort Resazurin as a 'smart' tracer for quantifying metabolically active transient storage in stream ecosystems
dc.creator.none.fl_str_mv Haggerty, Roy
Martí Roca, Eugènia
Argerich, Alba
Schiller Calle, Daniel von
Grimm, Nancy B.
author Haggerty, Roy
author_facet Haggerty, Roy
Martí Roca, Eugènia
Argerich, Alba
Schiller Calle, Daniel von
Grimm, Nancy B.
author_role author
author2 Martí Roca, Eugènia
Argerich, Alba
Schiller Calle, Daniel von
Grimm, Nancy B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biogeoquímica
Ecologia fluvial
Cursos d'aigua
Biogeochemistry
Stream ecology
Rivers
topic Biogeoquímica
Ecologia fluvial
Cursos d'aigua
Biogeochemistry
Stream ecology
Rivers
description We propose the experimental use of resazurin (Raz) and develop a metabolically active transient storage (MATS) model to include processes that may provide additional information on transient storage from a biogeochemical perspective in stream ecosystems. Raz is a phenoxazine compound that reduces irreversibly to resorufin (Rru) in the presence of aerobic bacteria. Raz was added as a stream tracer to a 128‐m reach of the forested second‐order Riera de Santa Fe del Montseny (Catalonia, NE Spain), along with a conservative tracer, NaCl. Raz was transformed to Rru at a rate of 0.81 h−1 in the hyporheic zone and only at a rate of 9.9 × 10−4 h−1 in the stream surface channel. Raz transformation and decay and Rru production and decay were both correlated with O2 consumption measured at wells. The ratio of Raz to Rru concentration at the bottom of the reach was moderately correlated with instantaneous rates of net ecosystem production (NEP) measured over the whole reach. Data for Raz, Rru, and chloride were well fitted with the MATS model. The results from this study suggest that Raz transformation to Rru can be used as a 'smart' tracer to detect metabolic activity, specifically aerobic respiration, associated with transient storage zones in stream ecosystems. Therefore, the Raz‐Rru system can provide an assessment of the amount of transient storage that is metabolically active, an assessment that complements the physical characterization of transient storage obtained from conventional hydrologic tracers. The use of both physical and metabolic parameters of transient storage obtained with these tracers may increase our understanding of the relevance of transient storage on stream biogeochemical processes at whole reach scale, as well as the contribution of the different transient storage compartments to these processes.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173291
url https://hdl.handle.net/2445/173291
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1029/2008JG000942
Journal of Geophysical Research, 2009, vol. 114, num. G03014, p. 1-14
https://doi.org/10.1029/2008JG000942
dc.rights.none.fl_str_mv (c) American Geophysical Union (AGU) , 2009
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Geophysical Union (AGU) , 2009
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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