Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding

Our study aimed to compare the effectiveness of microscopic and DNA metabarcoding approaches in assessing the abundance and diversity of epilithic diatoms, while also exploring the suitability of metabarcoding as a reliable biomonitoring tool in Andean freshwater ecosystems. For this, 16 samples fro...

ver descrição completa

Detalhes bibliográficos
Autores: Castillejo Pons, Pablo, Lobo, Eduardo, Heinrich, Carla, Perdiguero Jiménez, Pedro, Frandsen, Paul, Rios-Touma, Blanca, Ballesteros Redondo, María Isabel
Tipo de documento: conjunto de datos
Data de publicação:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/107425
Acesso em linha:https://hdl.handle.net/20.500.14352/107425
Access Level:Acceso aberto
Palavra-chave:577.2
Biología molecular (Biología)
2415 Biología Molecular
id ES_0290cb87209bc3d77d55fdd4a329ddbd
oai_identifier_str oai:docta.ucm.es:20.500.14352/107425
network_acronym_str ES
network_name_str España
repository_id_str
spelling Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA MetabarcodingCastillejo Pons, PabloLobo, EduardoHeinrich, CarlaPerdiguero Jiménez, PedroFrandsen, PaulRios-Touma, BlancaBallesteros Redondo, María Isabel577.2Biología molecular (Biología)2415 Biología MolecularOur study aimed to compare the effectiveness of microscopic and DNA metabarcoding approaches in assessing the abundance and diversity of epilithic diatoms, while also exploring the suitability of metabarcoding as a reliable biomonitoring tool in Andean freshwater ecosystems. For this, 16 samples from the Guayllabamba River Basin in Ecuador were analyzed. Microscopic analysis involved the taxonomic identification of at least 600 valves under a light microscope. The molecular approach involved amplifying a rbcL region of approximately 1500 bp and conducting high-throughput DNA sequencing using Oxford Nanopore technology.Universidad Complutense de Madrid20232023-01-0120232023-01-01datasethttp://purl.org/coar/resource_type/c_ddb1info:eu-repo/semantics/datasetapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamhttps://hdl.handle.net/20.500.14352/107425reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1074252026-06-02T12:44:21Z
dc.title.none.fl_str_mv Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
title Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
spellingShingle Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
Castillejo Pons, Pablo
577.2
Biología molecular (Biología)
2415 Biología Molecular
title_short Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
title_full Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
title_fullStr Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
title_full_unstemmed Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
title_sort Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
dc.creator.none.fl_str_mv Castillejo Pons, Pablo
Lobo, Eduardo
Heinrich, Carla
Perdiguero Jiménez, Pedro
Frandsen, Paul
Rios-Touma, Blanca
Ballesteros Redondo, María Isabel
author Castillejo Pons, Pablo
author_facet Castillejo Pons, Pablo
Lobo, Eduardo
Heinrich, Carla
Perdiguero Jiménez, Pedro
Frandsen, Paul
Rios-Touma, Blanca
Ballesteros Redondo, María Isabel
author_role author
author2 Lobo, Eduardo
Heinrich, Carla
Perdiguero Jiménez, Pedro
Frandsen, Paul
Rios-Touma, Blanca
Ballesteros Redondo, María Isabel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.2
Biología molecular (Biología)
2415 Biología Molecular
topic 577.2
Biología molecular (Biología)
2415 Biología Molecular
description Our study aimed to compare the effectiveness of microscopic and DNA metabarcoding approaches in assessing the abundance and diversity of epilithic diatoms, while also exploring the suitability of metabarcoding as a reliable biomonitoring tool in Andean freshwater ecosystems. For this, 16 samples from the Guayllabamba River Basin in Ecuador were analyzed. Microscopic analysis involved the taxonomic identification of at least 600 valves under a light microscope. The molecular approach involved amplifying a rbcL region of approximately 1500 bp and conducting high-throughput DNA sequencing using Oxford Nanopore technology.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv dataset
http://purl.org/coar/resource_type/c_ddb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/107425
url https://hdl.handle.net/20.500.14352/107425
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
application/octet-stream
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402663879180288
score 15,812429

Registros relacionados