The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics

Introduction: Preeclampsia is a multi-system disorder unique to pregnancy responsible for a great part of maternal and perinatal morbidity and mortality. The precise pathogenesis of this complex disorder is still unrevealed. Methods: We examined the pathophysiological pathways involved in early-onse...

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Autores: Youssef, Lina, Crovetto, Francesca, Vasco Simoes, Rui, Miranda, Jezid, Paules, Cristina, Blasco, Miquel, Palomo, Marta, García Calderó, Héctor, Tura-Ceide, Olga, Dantas, Ana Paula, Hernandez Gea, Virginia, Herrero, Pol, Canela i Canela, Núria, Campistol Plana, Josep M., García Pagán, Juan Carlos, Diaz Ricart, M. Isabel, Gratacós Freixas, Eduardo, Crispi Brillas, Fàtima
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/186808
Acceso en línea:https://hdl.handle.net/2445/186808
Access Level:acceso abierto
Palabra clave:Preeclàmpsia
Metabolòmica
Creixement fetal
Preeclampsia
Metabolomics
Fetal growth
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spelling The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by MetabolomicsYoussef, LinaCrovetto, FrancescaVasco Simoes, RuiMiranda, JezidPaules, CristinaBlasco, MiquelPalomo, MartaGarcía Calderó, HéctorTura-Ceide, OlgaDantas, Ana PaulaHernandez Gea, VirginiaHerrero, PolCanela i Canela, NúriaCampistol Plana, Josep M.García Pagán, Juan CarlosDiaz Ricart, M. IsabelGratacós Freixas, EduardoCrispi Brillas, FàtimaPreeclàmpsiaMetabolòmicaCreixement fetalPreeclampsiaMetabolomicsFetal growthIntroduction: Preeclampsia is a multi-system disorder unique to pregnancy responsible for a great part of maternal and perinatal morbidity and mortality. The precise pathogenesis of this complex disorder is still unrevealed. Methods: We examined the pathophysiological pathways involved in early-onset preeclampsia, a specific subgroup representing its most severe presentation, using LC-MS/MS metabolomic analysis based on multi-level extraction of lipids and small metabolites from maternal blood samples, collected at the time of diagnosis from 14 preeclamptic and six matched healthy pregnancies. Statistical analysis comprised multivariate and univariate approaches with the application of over representation analysis to identify differential pathways. Results: A clear difference between preeclamptic and control pregnancies was observed in principal component analysis. Supervised multivariate analysis using orthogonal partial least square discriminant analysis provided a robust model with goodness of fit (R2X = 0.91, p = 0.002) and predictive ability (Q2Y = 0.72, p < 0.001). Finally, univariate analysis followed by 5% false discovery rate correction indicated 82 metabolites significantly altered, corresponding to six overrepresented pathways: (1) aminoacyl-tRNA biosynthesis; (2) arginine biosynthesis; (3) alanine, aspartate and glutamate metabolism; (4) D-glutamine and D-glutamate metabolism; (5) arginine and proline metabolism; and (6) histidine metabolism. Conclusion: Metabolomic analysis focusing specifically on the early-onset severe form of preeclampsia reveals the interplay between pathophysiological pathways involved in this form. Future studies are required to explore new therapeutic approaches targeting these altered metabolic pathways in early-onset preeclampsiaMDPI2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion21 p.application/pdfhttps://hdl.handle.net/2445/186808Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/life12010086Life, 2022, vol. 12, num. 1, p. 86https://doi.org/10.3390/life12010086cc-by (c) Youssef, Lina et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1868082026-05-29T05:05:01Z
dc.title.none.fl_str_mv The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
title The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
spellingShingle The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
Youssef, Lina
Preeclàmpsia
Metabolòmica
Creixement fetal
Preeclampsia
Metabolomics
Fetal growth
title_short The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
title_full The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
title_fullStr The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
title_full_unstemmed The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
title_sort The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
dc.creator.none.fl_str_mv Youssef, Lina
Crovetto, Francesca
Vasco Simoes, Rui
Miranda, Jezid
Paules, Cristina
Blasco, Miquel
Palomo, Marta
García Calderó, Héctor
Tura-Ceide, Olga
Dantas, Ana Paula
Hernandez Gea, Virginia
Herrero, Pol
Canela i Canela, Núria
Campistol Plana, Josep M.
García Pagán, Juan Carlos
Diaz Ricart, M. Isabel
Gratacós Freixas, Eduardo
Crispi Brillas, Fàtima
author Youssef, Lina
author_facet Youssef, Lina
Crovetto, Francesca
Vasco Simoes, Rui
Miranda, Jezid
Paules, Cristina
Blasco, Miquel
Palomo, Marta
García Calderó, Héctor
Tura-Ceide, Olga
Dantas, Ana Paula
Hernandez Gea, Virginia
Herrero, Pol
Canela i Canela, Núria
Campistol Plana, Josep M.
García Pagán, Juan Carlos
Diaz Ricart, M. Isabel
Gratacós Freixas, Eduardo
Crispi Brillas, Fàtima
author_role author
author2 Crovetto, Francesca
Vasco Simoes, Rui
Miranda, Jezid
Paules, Cristina
Blasco, Miquel
Palomo, Marta
García Calderó, Héctor
Tura-Ceide, Olga
Dantas, Ana Paula
Hernandez Gea, Virginia
Herrero, Pol
Canela i Canela, Núria
Campistol Plana, Josep M.
García Pagán, Juan Carlos
Diaz Ricart, M. Isabel
Gratacós Freixas, Eduardo
Crispi Brillas, Fàtima
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Preeclàmpsia
Metabolòmica
Creixement fetal
Preeclampsia
Metabolomics
Fetal growth
topic Preeclàmpsia
Metabolòmica
Creixement fetal
Preeclampsia
Metabolomics
Fetal growth
description Introduction: Preeclampsia is a multi-system disorder unique to pregnancy responsible for a great part of maternal and perinatal morbidity and mortality. The precise pathogenesis of this complex disorder is still unrevealed. Methods: We examined the pathophysiological pathways involved in early-onset preeclampsia, a specific subgroup representing its most severe presentation, using LC-MS/MS metabolomic analysis based on multi-level extraction of lipids and small metabolites from maternal blood samples, collected at the time of diagnosis from 14 preeclamptic and six matched healthy pregnancies. Statistical analysis comprised multivariate and univariate approaches with the application of over representation analysis to identify differential pathways. Results: A clear difference between preeclamptic and control pregnancies was observed in principal component analysis. Supervised multivariate analysis using orthogonal partial least square discriminant analysis provided a robust model with goodness of fit (R2X = 0.91, p = 0.002) and predictive ability (Q2Y = 0.72, p < 0.001). Finally, univariate analysis followed by 5% false discovery rate correction indicated 82 metabolites significantly altered, corresponding to six overrepresented pathways: (1) aminoacyl-tRNA biosynthesis; (2) arginine biosynthesis; (3) alanine, aspartate and glutamate metabolism; (4) D-glutamine and D-glutamate metabolism; (5) arginine and proline metabolism; and (6) histidine metabolism. Conclusion: Metabolomic analysis focusing specifically on the early-onset severe form of preeclampsia reveals the interplay between pathophysiological pathways involved in this form. Future studies are required to explore new therapeutic approaches targeting these altered metabolic pathways in early-onset preeclampsia
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186808
url https://hdl.handle.net/2445/186808
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/life12010086
Life, 2022, vol. 12, num. 1, p. 86
https://doi.org/10.3390/life12010086
dc.rights.none.fl_str_mv cc-by (c) Youssef, Lina et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Youssef, Lina et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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