Control of skin damages caused by oxidative stress using mangiferin and naringin co-loaded in phospholipid vesicles

[EN] Mangiferin and naringin, two naturally occurring antioxidant molecules, were co-loaded in phospholipid vesicles designed for skin delivery. Ultradeformable-liposomes containing tween 80 as edge activator, were used as basic formulation, which was modified adding glycerol (glycerosomes) or a mix...

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Detalles Bibliográficos
Autores: Pleguezuelos-Villa, María, Castangia, Ines, Díez-Sales, Octavio, Manca, Maria Letizia, Manconi, Maria, Ruiz Sauri, Amparo, Talens-Visconti, Raquel, Nacher, Amparo
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/204601
Acceso en línea:https://riunet.upv.es/handle/10251/204601
Access Level:acceso abierto
Palabra clave:Mangiferin
Naringin
Phospholipid vesicles
Sodium hyaluronate
Rheology
Oxidative stress
Skin delivery
Descripción
Sumario:[EN] Mangiferin and naringin, two naturally occurring antioxidant molecules, were co-loaded in phospholipid vesicles designed for skin delivery. Ultradeformable-liposomes containing tween 80 as edge activator, were used as basic formulation, which was modified adding glycerol (glycerosomes) or a mixture of glycerol and ethanol (etglycerosomes) and further enriched with a polymer, sodium hyaluronate (glycerohyalurosomes and etglycerohyalurosomes), to evaluate the role of vesicle composition on their features and performances. Mean dimeter, polydispersity index and zeta potential of prepared vesicles were measured along with their stabilitcay on storage for 90 days, rheological behavior and suitability as systems for the delivery of these active molecules into and through the skin. Vesicles enriched with sodium hyaluronate were the most stable and the smallest and favored the deposition of both mangiferin and naringin in the whole skin, in a better extent than those without polymer. All the vesicles were highly biocompatible and capable of protecting fibroblasts against hydrogen peroxide-induced oxidative damages in vitro. Once more, glycerohyalurosomes and et-glycerohyalurosomes where those which improved the most the beneficial effect of mangiferin and naringin, as they were capable of effectively counteracting the formation of skin lesion, or even promoting the wound healing, thanks to their greater ability to inhibit both myeloperoxydase activity and oedema formation in vivo in a model mouse in which wound was induced using phorbol acetate.