An Integrative Approach to Analyze Seed Germination in Brassica napus

24 Pág.

Detalles Bibliográficos
Autores: Boter, Marta, Calleja-Cabrera, Julián, Carrera-Castaño, Gerardo, Wagner, Geoffrey, Hatzig, Sarah Vanessa, Snowdon, Rod, Legoahec, Laurie, Bianchetti, Grégoire, Bouchereau, Alain, Nesi, Nathalie, Pernas Ochoa, Mónica, Oñate-Sánchez, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/365704
Acceso en línea:http://hdl.handle.net/10261/365704
https://api.elsevier.com/content/abstract/scopus_id/85074696963
Access Level:acceso abierto
Palabra clave:Crop yield
Germination
Hormonal pathways
Metabolism
Oilseed rape (Brassica napus)
Protein interaction
Seed traits
Transcriptomic
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oai_identifier_str oai:digital.csic.es:10261/365704
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv An Integrative Approach to Analyze Seed Germination in Brassica napus
title An Integrative Approach to Analyze Seed Germination in Brassica napus
spellingShingle An Integrative Approach to Analyze Seed Germination in Brassica napus
Boter, Marta
Crop yield
Germination
Hormonal pathways
Metabolism
Oilseed rape (Brassica napus)
Protein interaction
Seed traits
Transcriptomic
title_short An Integrative Approach to Analyze Seed Germination in Brassica napus
title_full An Integrative Approach to Analyze Seed Germination in Brassica napus
title_fullStr An Integrative Approach to Analyze Seed Germination in Brassica napus
title_full_unstemmed An Integrative Approach to Analyze Seed Germination in Brassica napus
title_sort An Integrative Approach to Analyze Seed Germination in Brassica napus
dc.creator.none.fl_str_mv Boter, Marta
Calleja-Cabrera, Julián
Carrera-Castaño, Gerardo
Wagner, Geoffrey
Hatzig, Sarah Vanessa
Snowdon, Rod
Legoahec, Laurie
Bianchetti, Grégoire
Bouchereau, Alain
Nesi, Nathalie
Pernas Ochoa, Mónica
Oñate-Sánchez, Luis
author Boter, Marta
author_facet Boter, Marta
Calleja-Cabrera, Julián
Carrera-Castaño, Gerardo
Wagner, Geoffrey
Hatzig, Sarah Vanessa
Snowdon, Rod
Legoahec, Laurie
Bianchetti, Grégoire
Bouchereau, Alain
Nesi, Nathalie
Pernas Ochoa, Mónica
Oñate-Sánchez, Luis
author_role author
author2 Calleja-Cabrera, Julián
Carrera-Castaño, Gerardo
Wagner, Geoffrey
Hatzig, Sarah Vanessa
Snowdon, Rod
Legoahec, Laurie
Bianchetti, Grégoire
Bouchereau, Alain
Nesi, Nathalie
Pernas Ochoa, Mónica
Oñate-Sánchez, Luis
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad Politécnica de Madrid
Boter, Marta [0000-0001-7489-2791]
Calleja-Cabrera, Julián [0000-0003-0510-4741]
Carrera-Castaño, Gerardo [0000-0002-0393-3186]
Snowdon, Rod [0000-0001-5577-7616]
Bianchetti, Grégoire [0000-0003-1192-982X]
Bouchereau, Alain [0000-0002-2473-3773]
Nesi, Nathalie [0000-0002-2552-472X]
Pernas Ochoa, Mónica [0000-0002-6521-9164]
Oñate-Sánchez, Luis [0000-0001-7047-5739]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Crop yield
Germination
Hormonal pathways
Metabolism
Oilseed rape (Brassica napus)
Protein interaction
Seed traits
Transcriptomic
topic Crop yield
Germination
Hormonal pathways
Metabolism
Oilseed rape (Brassica napus)
Protein interaction
Seed traits
Transcriptomic
description 24 Pág.
publishDate 2019
dc.date.none.fl_str_mv 2019
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/365704
https://api.elsevier.com/content/abstract/scopus_id/85074696963
url http://hdl.handle.net/10261/365704
https://api.elsevier.com/content/abstract/scopus_id/85074696963
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICIU//BIO2016- 77840-R
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://doi.org/10.3389/fpls.2019.01342
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling An Integrative Approach to Analyze Seed Germination in Brassica napusBoter, MartaCalleja-Cabrera, JuliánCarrera-Castaño, GerardoWagner, GeoffreyHatzig, Sarah VanessaSnowdon, RodLegoahec, LaurieBianchetti, GrégoireBouchereau, AlainNesi, NathaliePernas Ochoa, MónicaOñate-Sánchez, LuisCrop yieldGerminationHormonal pathwaysMetabolismOilseed rape (Brassica napus)Protein interactionSeed traitsTranscriptomic24 Pág.Seed germination is a complex trait determined by the interaction of hormonal, metabolic, genetic, and environmental components. Variability of this trait in crops has a big impact on seedling establishment and yield in the field. Classical studies of this trait in crops have focused mainly on the analyses of one level of regulation in the cascade of events leading to seed germination. We have carried out an integrative and extensive approach to deepen our understanding of seed germination in Brassica napus by generating transcriptomic, metabolic, and hormonal data at different stages upon seed imbibition. Deep phenotyping of different seed germination-associated traits in six winter-type B. napus accessions has revealed that seed germination kinetics, in particular seed germination speed, are major contributors to the variability of this trait. Metabolic profiling of these accessions has allowed us to describe a common pattern of metabolic change and to identify the levels of malate and aspartate metabolites as putative metabolic markers to estimate germination performance. Additionally, analysis of seed content of different hormones suggests that hormonal balance between ABA, GA, and IAA at crucial time points during this process might underlie seed germination differences in these accessions. In this study, we have also defined the major transcriptome changes accompanying the germination process in B. napus. Furthermore, we have observed that earlier activation of key germination regulatory genes seems to generate the differences in germination speed observed between accessions in B. napus. Finally, we have found that protein-protein interactions between some of these key regulator are conserved in B. napus, suggesting a shared regulatory network with other plant species. Altogether, our results provide a comprehensive and detailed picture of seed germination dynamics in oilseed rape. This new framework will be extremely valuable not only to evaluate germination performance of B. napus accessions but also to identify key targets for crop improvement in this important process.This work was supported with a grant to LO-S by the Spanish Ministry of Science and Universities of Spain (BIO2016- 77840-R) and to MP by FP7. FACCE-JPI-ERA-NET+ CLIMATE SMART AGRICULTURE (ERA46-SYBRACLYM). RS acknowledges funding from BMBF grants 031A549 (SYBRACLIM) and 0315910 (CONVIGOUR). NN, AB, LL, and GB acknowledge funding from the transnational PLANT-KBBE cooperation project CONVIGOUR with funding from ANR (France). MB was supported by a postdoctoral research fellowship and JC-C by a PhD contract (FPI), both funded by grant SEV-2016-0672 to the CBGP (Centre of Excellence Severo Ochoa Program of the Agencia Estatal de Investigación, Spain). GC-C was supported by a PhD contract by Universidad Politécnica de Madrid (UPM; programa propio).Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad Politécnica de MadridBoter, Marta [0000-0001-7489-2791]Calleja-Cabrera, Julián [0000-0003-0510-4741]Carrera-Castaño, Gerardo [0000-0002-0393-3186]Snowdon, Rod [0000-0001-5577-7616]Bianchetti, Grégoire [0000-0003-1192-982X]Bouchereau, Alain [0000-0002-2473-3773]Nesi, Nathalie [0000-0002-2552-472X]Pernas Ochoa, Mónica [0000-0002-6521-9164]Oñate-Sánchez, Luis [0000-0001-7047-5739]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/365704https://api.elsevier.com/content/abstract/scopus_id/85074696963reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICIU//BIO2016- 77840-RCentro de Biotecnología y Genómica de Plantas (CBGP)https://doi.org/10.3389/fpls.2019.01342Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3657042026-05-22T06:33:51Z
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