Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations

© 2023 The Authors.

Detalles Bibliográficos
Autores: Merenciano, Miriam, Aguilera, Laura, González Pérez, Josefa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/348815
Acceso en línea:http://hdl.handle.net/10261/348815
Access Level:acceso abierto
Palabra clave:CRISPR
Evolutionary Biology
Genetics
Model Organisms
Molecular Biology
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spelling Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populationsMerenciano, MiriamAguilera, LauraGonzález Pérez, JosefaCRISPREvolutionary BiologyGeneticsModel OrganismsMolecular Biology© 2023 The Authors.[Summary] We present a protocol for generating a precise deletion, without altering the genetic background of the strain, of a transposable element (TE) in a natural population of Drosophila melanogaster using two steps of CRISPR-Cas9 homology-directed repair. We describe steps for replacing the TE by a fluorescent marker and for subsequent marker removal using single-guide RNAs, repair plasmids, and microinjection. We also detail steps for screening the deletion of the TE and generating a homozygous mutant strain.This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (H2020-ERC-2014-CoG-647900).Peer reviewedCell PressEuropean Research CouncilEuropean CommissionMerenciano, Miriam [0000-0001-8592-949X]Aguilera, Laura [0000-0002-8631-8778]González Pérez, Josefa [0000-0001-9824-027X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/348815reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/647900https://doi.org/10.1016/j.xpro.2023.102501Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3488152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
title Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
spellingShingle Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
Merenciano, Miriam
CRISPR
Evolutionary Biology
Genetics
Model Organisms
Molecular Biology
title_short Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
title_full Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
title_fullStr Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
title_full_unstemmed Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
title_sort Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
dc.creator.none.fl_str_mv Merenciano, Miriam
Aguilera, Laura
González Pérez, Josefa
author Merenciano, Miriam
author_facet Merenciano, Miriam
Aguilera, Laura
González Pérez, Josefa
author_role author
author2 Aguilera, Laura
González Pérez, Josefa
author2_role author
author
dc.contributor.none.fl_str_mv European Research Council
European Commission
Merenciano, Miriam [0000-0001-8592-949X]
Aguilera, Laura [0000-0002-8631-8778]
González Pérez, Josefa [0000-0001-9824-027X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CRISPR
Evolutionary Biology
Genetics
Model Organisms
Molecular Biology
topic CRISPR
Evolutionary Biology
Genetics
Model Organisms
Molecular Biology
description © 2023 The Authors.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/348815
url http://hdl.handle.net/10261/348815
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/647900
https://doi.org/10.1016/j.xpro.2023.102501
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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