Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions

An increasing number of biocatalytic oxidation reactions rely on H2O2 as a clean oxidant. The poor robustness of most enzymes towards H2O2, however, necessitates more efficient systems for in situ H2O2 generation. In analogy to the well‐known formate dehydrogenase to promote NADH‐dependent reactions...

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Detalles Bibliográficos
Autores: Tieves, Florian, Willot, S.J.P., Van Schie, M. M. C. H., Rauch, M.C.R., Younes, S.H.H., Zhang, W., Dong, J.J., Gómez de Santos, Patricia, Robbins, J.M., Bommarius, B., Alcalde Galeote, Miguel, Bommarius, A.S., Hollmann, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/214478
Acceso en línea:http://hdl.handle.net/10261/214478
Access Level:acceso abierto
Palabra clave:Biiocatalysis
Formate oxidase
Hydrogen peroxide
Oxidation
Oxyfunctionalisation
Descripción
Sumario:An increasing number of biocatalytic oxidation reactions rely on H2O2 as a clean oxidant. The poor robustness of most enzymes towards H2O2, however, necessitates more efficient systems for in situ H2O2 generation. In analogy to the well‐known formate dehydrogenase to promote NADH‐dependent reactions, we here propose employing formate oxidase (FOx) to promote H2O2‐dependent enzymatic oxidation reactions. Even under non‐optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

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