FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations

Objective To determine the role of enterokine FGF15/19 in adipose tissue thermogenic adaptations. Methods Circulating FGF19 and gene expression (qRT-PCR) levels were assessed in subcutaneous adipose tissue from obese human patients. Effects of experimentally increased FGF15 and FGF19 levels in vivo...

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Detalles Bibliográficos
Autores: Morón-Ros, Samantha, Uriarte, Iker, Berasain, Carmen, Avila, Matías Antonio, Sabater Masdeu, Mònica, Moreno Navarrete, José María, Fernández-Real Lemos, José Manuel, Giralt i Oms, Marta, Villarroya, Francesc, Gavaldà i Navarro, Aleix
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/21300
Acceso en línea:http://hdl.handle.net/10256/21300
Access Level:acceso abierto
Palabra clave:Teixit adipós
Adipose tissues
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spelling FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptationsMorón-Ros, SamanthaUriarte, IkerBerasain, CarmenAvila, Matías AntonioSabater Masdeu, MònicaMoreno Navarrete, José MaríaFernández-Real Lemos, José ManuelGiralt i Oms, MartaVillarroya, FrancescGavaldà i Navarro, AleixTeixit adipósAdipose tissuesObjective To determine the role of enterokine FGF15/19 in adipose tissue thermogenic adaptations. Methods Circulating FGF19 and gene expression (qRT-PCR) levels were assessed in subcutaneous adipose tissue from obese human patients. Effects of experimentally increased FGF15 and FGF19 levels in vivo were determined in mice using adenoviral and adeno-associated vectors. Adipose tissues were characterized in FGF15-null mice under distinct cold-related thermogenic challenges. The analyses spanned metabolic profiling, tissue characterization, histology, gene expression, and immunoblot assays. Results In humans, FGF19 levels are directly associated with UCP1 gene expression in subcutaneous adipose tissue. Experimental increases in FGF15 or FGF19 induced white fat browning in mice as demonstrated by the appearance of multilocular beige cells and markers indicative of a beige phenotype, including increased UCP1 protein levels. Mice lacking FGF15 showed markedly impaired white adipose tissue browning and a mild reduction in parameters indicative of BAT activity in response to cold-induced environmental thermogenic challenges. This was concomitant with signs of altered systemic metabolism, such as reduced glucose tolerance and impaired cold-induced insulin sensitizationFunding from the Ministry of Science and Innovation (SAF2017-85722R); Health Research Fund, Carlos III Health Institute (PI17-00420), co-financed by the European Regional Development Fund (ERDF); the Marató de TV3 Foundation (201612-30/31); the Fundación Bancaria La Caixa (Hepacare Project); the M. Torres Foundation; and the Eugenio Rodriguez Pascual Foundation (to IU, CB, and MAA) is acknowledgedElsevier2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/21300Molecular Metabolism, 2021, vol. 43, art.núm. 101113Articles publicats (IdIBGi)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molmet.2020.101113info:eu-repo/semantics/altIdentifier/eissn/2212-8778Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/213002026-05-29T05:05:01Z
dc.title.none.fl_str_mv FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
title FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
spellingShingle FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
Morón-Ros, Samantha
Teixit adipós
Adipose tissues
title_short FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
title_full FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
title_fullStr FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
title_full_unstemmed FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
title_sort FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations
dc.creator.none.fl_str_mv Morón-Ros, Samantha
Uriarte, Iker
Berasain, Carmen
Avila, Matías Antonio
Sabater Masdeu, Mònica
Moreno Navarrete, José María
Fernández-Real Lemos, José Manuel
Giralt i Oms, Marta
Villarroya, Francesc
Gavaldà i Navarro, Aleix
author Morón-Ros, Samantha
author_facet Morón-Ros, Samantha
Uriarte, Iker
Berasain, Carmen
Avila, Matías Antonio
Sabater Masdeu, Mònica
Moreno Navarrete, José María
Fernández-Real Lemos, José Manuel
Giralt i Oms, Marta
Villarroya, Francesc
Gavaldà i Navarro, Aleix
author_role author
author2 Uriarte, Iker
Berasain, Carmen
Avila, Matías Antonio
Sabater Masdeu, Mònica
Moreno Navarrete, José María
Fernández-Real Lemos, José Manuel
Giralt i Oms, Marta
Villarroya, Francesc
Gavaldà i Navarro, Aleix
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Teixit adipós
Adipose tissues
topic Teixit adipós
Adipose tissues
description Objective To determine the role of enterokine FGF15/19 in adipose tissue thermogenic adaptations. Methods Circulating FGF19 and gene expression (qRT-PCR) levels were assessed in subcutaneous adipose tissue from obese human patients. Effects of experimentally increased FGF15 and FGF19 levels in vivo were determined in mice using adenoviral and adeno-associated vectors. Adipose tissues were characterized in FGF15-null mice under distinct cold-related thermogenic challenges. The analyses spanned metabolic profiling, tissue characterization, histology, gene expression, and immunoblot assays. Results In humans, FGF19 levels are directly associated with UCP1 gene expression in subcutaneous adipose tissue. Experimental increases in FGF15 or FGF19 induced white fat browning in mice as demonstrated by the appearance of multilocular beige cells and markers indicative of a beige phenotype, including increased UCP1 protein levels. Mice lacking FGF15 showed markedly impaired white adipose tissue browning and a mild reduction in parameters indicative of BAT activity in response to cold-induced environmental thermogenic challenges. This was concomitant with signs of altered systemic metabolism, such as reduced glucose tolerance and impaired cold-induced insulin sensitization
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/21300
url http://hdl.handle.net/10256/21300
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molmet.2020.101113
info:eu-repo/semantics/altIdentifier/eissn/2212-8778
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Molecular Metabolism, 2021, vol. 43, art.núm. 101113
Articles publicats (IdIBGi)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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