Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture
An anaerobic stable mixed culture dominated by bacteria belonging to the genera Dehalobacterium, Acetobacterium, Desulfovibrio, and Wolinella was used as a model to study the microbial interactions during DCM degradation. Physiological studies indicated that DCM was degraded in this mixed culture at...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:212957 |
| Acesso em linha: | https://ddd.uab.cat/record/212957 https://dx.doi.org/urn:doi:10.1016/j.chemosphere.2019.124877 |
| Access Level: | acceso abierto |
| Palavra-chave: | Dehalobacterium Dichloromethane Co-contaminants Inhibition Bioremediation |
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Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading cultureTrueba-Santiso, Alba|||0000-0001-9730-7321Fernandez Verdejo, David Juan|||0000-0002-3234-4006Marco Rius, IreneSoder-Walz, Jesica M.|||0000-0002-5253-9898Casabella, OriolVicent i Huguet, Teresa|||0000-0002-6547-7358Marco Urrea, Ernest|||0000-0002-8033-6553DehalobacteriumDichloromethaneCo-contaminantsInhibitionBioremediationAn anaerobic stable mixed culture dominated by bacteria belonging to the genera Dehalobacterium, Acetobacterium, Desulfovibrio, and Wolinella was used as a model to study the microbial interactions during DCM degradation. Physiological studies indicated that DCM was degraded in this mixed culture at least in a three-step process: i) fermentation of DCM to acetate and formate, ii) formate oxidation to CO₂ and H₂, and iii) H₂/CO₂ reductive acetogenesis. The 16S rRNA gene sequencing of cultures enriched with formate or H₂ showed that Desulfovibrio was the dominant population followed by Acetobacterium, but sequences representing Dehalobacterium were only present in cultures amended with DCM. Nuclear magnetic resonance analyses confirmed that acetate produced from ¹³C-labelled DCM was marked at the methyl ([2-¹³C]acetate), carboxyl ([1-¹³C]acetate), and both ([1,2-¹³C]acetate) positions, which is in accordance to acetate formed by both direct DCM fermentation and H₂/CO₂ acetogenesis. The inhibitory effect of ten different co-contaminants frequently detected in groundwaters on DCM degradation was also investigated. Complete inhibition of DCM degradation was observed when chloroform, perfluorooctanesulfonic acid, and diuron were added at 838, 400, and 107 μM, respectively. However, the inhibited cultures recovered the DCM degradation capability when transferred to fresh medium without co-contaminants. Findings derived from this work are of significant relevance to provide a better understanding of the synergistic interactions among bacteria to accomplish DCM degradation as well as to predict the effect of co-contaminants during anaerobic DCM bioremediation in groundwater. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/212957https://dx.doi.org/urn:doi:10.1016/j.chemosphere.2019.124877reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 CTM2016-75587-C2-1-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-070817Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTM2013-48545-C2-1-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-14open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2129572026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| title |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| spellingShingle |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture Trueba-Santiso, Alba|||0000-0001-9730-7321 Dehalobacterium Dichloromethane Co-contaminants Inhibition Bioremediation |
| title_short |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| title_full |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| title_fullStr |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| title_full_unstemmed |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| title_sort |
Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture |
| dc.creator.none.fl_str_mv |
Trueba-Santiso, Alba|||0000-0001-9730-7321 Fernandez Verdejo, David Juan|||0000-0002-3234-4006 Marco Rius, Irene Soder-Walz, Jesica M.|||0000-0002-5253-9898 Casabella, Oriol Vicent i Huguet, Teresa|||0000-0002-6547-7358 Marco Urrea, Ernest|||0000-0002-8033-6553 |
| author |
Trueba-Santiso, Alba|||0000-0001-9730-7321 |
| author_facet |
Trueba-Santiso, Alba|||0000-0001-9730-7321 Fernandez Verdejo, David Juan|||0000-0002-3234-4006 Marco Rius, Irene Soder-Walz, Jesica M.|||0000-0002-5253-9898 Casabella, Oriol Vicent i Huguet, Teresa|||0000-0002-6547-7358 Marco Urrea, Ernest|||0000-0002-8033-6553 |
| author_role |
author |
| author2 |
Fernandez Verdejo, David Juan|||0000-0002-3234-4006 Marco Rius, Irene Soder-Walz, Jesica M.|||0000-0002-5253-9898 Casabella, Oriol Vicent i Huguet, Teresa|||0000-0002-6547-7358 Marco Urrea, Ernest|||0000-0002-8033-6553 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Dehalobacterium Dichloromethane Co-contaminants Inhibition Bioremediation |
| topic |
Dehalobacterium Dichloromethane Co-contaminants Inhibition Bioremediation |
| description |
An anaerobic stable mixed culture dominated by bacteria belonging to the genera Dehalobacterium, Acetobacterium, Desulfovibrio, and Wolinella was used as a model to study the microbial interactions during DCM degradation. Physiological studies indicated that DCM was degraded in this mixed culture at least in a three-step process: i) fermentation of DCM to acetate and formate, ii) formate oxidation to CO₂ and H₂, and iii) H₂/CO₂ reductive acetogenesis. The 16S rRNA gene sequencing of cultures enriched with formate or H₂ showed that Desulfovibrio was the dominant population followed by Acetobacterium, but sequences representing Dehalobacterium were only present in cultures amended with DCM. Nuclear magnetic resonance analyses confirmed that acetate produced from ¹³C-labelled DCM was marked at the methyl ([2-¹³C]acetate), carboxyl ([1-¹³C]acetate), and both ([1,2-¹³C]acetate) positions, which is in accordance to acetate formed by both direct DCM fermentation and H₂/CO₂ acetogenesis. The inhibitory effect of ten different co-contaminants frequently detected in groundwaters on DCM degradation was also investigated. Complete inhibition of DCM degradation was observed when chloroform, perfluorooctanesulfonic acid, and diuron were added at 838, 400, and 107 μM, respectively. However, the inhibited cultures recovered the DCM degradation capability when transferred to fresh medium without co-contaminants. Findings derived from this work are of significant relevance to provide a better understanding of the synergistic interactions among bacteria to accomplish DCM degradation as well as to predict the effect of co-contaminants during anaerobic DCM bioremediation in groundwater. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2 2020-01-01 2020 2020-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/212957 https://dx.doi.org/urn:doi:10.1016/j.chemosphere.2019.124877 |
| url |
https://ddd.uab.cat/record/212957 https://dx.doi.org/urn:doi:10.1016/j.chemosphere.2019.124877 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 CTM2016-75587-C2-1-R Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-070817 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTM2013-48545-C2-1-R Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-14 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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