C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression

Additional files: Applied bio systems codes.

Detalles Bibliográficos
Autores: Turu, Marta M., Slevin, Mark, Matou, Sabine, West, David, Rodríguez-Sinovas, Cristina, Luque, Ana, Grau-Olivares, Marta, Badimón Maestro, Lina, Martínez-González, José, Krupinski, Jerzy
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/8272
Acceso en línea:http://hdl.handle.net/10261/8272
Access Level:acceso abierto
Palabra clave:C-reactive protein
CRP
Vascular endothelial cells
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spelling C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expressionTuru, Marta M.Slevin, MarkMatou, SabineWest, DavidRodríguez-Sinovas, CristinaLuque, AnaGrau-Olivares, MartaBadimón Maestro, LinaMartínez-González, JoséKrupinski, JerzyC-reactive proteinCRPVascular endothelial cellsAdditional files: Applied bio systems codes.Background Formation of haemorrhagic neovessels in the intima of developing atherosclerotic plaques is thought to significantly contribute to plaque instability resulting in thrombosis. C-reactive protein (CRP) is an acute phase reactant whose expression in the vascular wall, in particular, in reactive plaque regions, and circulating levels increase in patients at high risk of cardiovascular events. Although CRP is known to induce a pro-inflammatory phenotype in endothelial cells (EC) a direct role on modulation of angiogenesis has not been established. Results Here, we show that CRP is a powerful inducer of angiogenesis in bovine aortic EC (BAEC) and human coronary artery EC (HCAEC). CRP, at concentrations corresponding to moderate/high risk (1–5 μg/ml), induced a significant increase in proliferation, migration and tube-like structure formation in vitro and stimulated blood vessel formation in the chick chorioallantoic membrane assay (CAM). CRP treated with detoxi-gel columns retained such effects. Western blotting showed that CRP increased activation of early response kinase-1/2 (ERK1/2), a key protein involved in EC mitogenesis. Furthermore, using TaqMan Low-density Arrays we identified key pro-angiogenic genes induced by CRP among them were vascular endothelial cell growth factor receptor-2 (VEGFR2/KDR), platelet-derived growth factor (PDGF-BB), notch family transcription factors (Notch1 and Notch3), cysteine-rich angiogenic inducer 61 (CYR61/CCN1) and inhibitor of DNA binding/differentiation-1 (ID1). Conclusion. This data suggests a role for CRP in direct stimulation of angiogenesis and therefore may be a mediator of neovessel formation in the intima of vulnerable plaques.This work was supported by grants: Marie Curie Reintegration Grant ERG-01152 and SAF 2006-07681 from the Ministerio de Educación y Ciencia (MEC) to JK.Peer reviewedBioMed Central200820082008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersion2324474 bytesapplication/pdfhttp://hdl.handle.net/10261/8272reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/1471-2121-9-47info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/82722026-05-22T06:33:51Z
dc.title.none.fl_str_mv C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
title C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
spellingShingle C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
Turu, Marta M.
C-reactive protein
CRP
Vascular endothelial cells
title_short C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
title_full C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
title_fullStr C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
title_full_unstemmed C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
title_sort C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression
dc.creator.none.fl_str_mv Turu, Marta M.
Slevin, Mark
Matou, Sabine
West, David
Rodríguez-Sinovas, Cristina
Luque, Ana
Grau-Olivares, Marta
Badimón Maestro, Lina
Martínez-González, José
Krupinski, Jerzy
author Turu, Marta M.
author_facet Turu, Marta M.
Slevin, Mark
Matou, Sabine
West, David
Rodríguez-Sinovas, Cristina
Luque, Ana
Grau-Olivares, Marta
Badimón Maestro, Lina
Martínez-González, José
Krupinski, Jerzy
author_role author
author2 Slevin, Mark
Matou, Sabine
West, David
Rodríguez-Sinovas, Cristina
Luque, Ana
Grau-Olivares, Marta
Badimón Maestro, Lina
Martínez-González, José
Krupinski, Jerzy
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv C-reactive protein
CRP
Vascular endothelial cells
topic C-reactive protein
CRP
Vascular endothelial cells
description Additional files: Applied bio systems codes.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/8272
url http://hdl.handle.net/10261/8272
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/1471-2121-9-47
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2324474 bytes
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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