Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)

Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen...

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Autores: Pérez Martínez, Isabel, Rodríguez Moreno, Luis, Lambertsen, Lotte, Matas Casado, Isabel María, Murillo Martínez, Jesús, Tegli, Stefania, Jiménez, Antonio J., Ramos, Cayo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/31931
Acceso en línea:https://hdl.handle.net/2454/31931
Access Level:acceso abierto
Palabra clave:Pseudomonas savastanoi pv. Savastanoi
Olive plants
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spelling Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)Pérez Martínez, IsabelRodríguez Moreno, LuisLambertsen, LotteMatas Casado, Isabel MaríaMurillo Martínez, JesúsTegli, StefaniaJiménez, Antonio J.Ramos, CayoPseudomonas savastanoi pv. SavastanoiOlive plantsPseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3 end of the hrpS gene to the 5 end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.This research has been supported by Spanish Plan Nacional I D I grants AGL2008-55311-CO2-01 and AGL2008-55311-CO2-02, cofinanced by FEDER, as well as by Integrated Action Spain-Italy HI01-81 and by Ente Cassa di Risparmio di Firenze (2007.1005 and 2008.1573). I.P.-M. was awarded a Spanish MEC fellowship (AP2002- 3800), and L.L. and I.M.M. were supported by Carlsberg Foundation (Denmark) grant ANS-1282/20 and the Ramón Areces Foundation (Spain), respectively.American Society for MicrobiologyProducción AgrariaNekazaritza Ekoizpena2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/31931reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2010, American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/319312026-06-17T12:41:47Z
dc.title.none.fl_str_mv Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
title Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
spellingShingle Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
Pérez Martínez, Isabel
Pseudomonas savastanoi pv. Savastanoi
Olive plants
title_short Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
title_full Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
title_fullStr Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
title_full_unstemmed Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
title_sort Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
dc.creator.none.fl_str_mv Pérez Martínez, Isabel
Rodríguez Moreno, Luis
Lambertsen, Lotte
Matas Casado, Isabel María
Murillo Martínez, Jesús
Tegli, Stefania
Jiménez, Antonio J.
Ramos, Cayo
author Pérez Martínez, Isabel
author_facet Pérez Martínez, Isabel
Rodríguez Moreno, Luis
Lambertsen, Lotte
Matas Casado, Isabel María
Murillo Martínez, Jesús
Tegli, Stefania
Jiménez, Antonio J.
Ramos, Cayo
author_role author
author2 Rodríguez Moreno, Luis
Lambertsen, Lotte
Matas Casado, Isabel María
Murillo Martínez, Jesús
Tegli, Stefania
Jiménez, Antonio J.
Ramos, Cayo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producción Agraria
Nekazaritza Ekoizpena
dc.subject.none.fl_str_mv Pseudomonas savastanoi pv. Savastanoi
Olive plants
topic Pseudomonas savastanoi pv. Savastanoi
Olive plants
description Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3 end of the hrpS gene to the 5 end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/31931
url https://hdl.handle.net/2454/31931
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2010, American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2010, American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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