Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor

© 2020 by the authors.

Detalles Bibliográficos
Autores: Calvo-Lozano, Olalla, Aviñó, Anna, Friaza Patiño, Vicente, Medina-Escuela, Alfonso, Huertas, César S., Calderón, Enrique J., Eritja Casadellà, Ramón, Lechuga, Laura M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215702
Acceso en línea:http://hdl.handle.net/10261/215702
Access Level:acceso abierto
Palabra clave:Pneumocystis jirovecii
Surface plasmon resonance
Optical biosensor
Clinical diagnosis
Triplex
DNA capture
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dc.title.none.fl_str_mv Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
spellingShingle Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
Calvo-Lozano, Olalla
Pneumocystis jirovecii
Surface plasmon resonance
Optical biosensor
Clinical diagnosis
Triplex
DNA capture
title_short Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_full Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_fullStr Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_full_unstemmed Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_sort Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
dc.creator.none.fl_str_mv Calvo-Lozano, Olalla
Aviñó, Anna
Friaza Patiño, Vicente
Medina-Escuela, Alfonso
Huertas, César S.
Calderón, Enrique J.
Eritja Casadellà, Ramón
Lechuga, Laura M.
author Calvo-Lozano, Olalla
author_facet Calvo-Lozano, Olalla
Aviñó, Anna
Friaza Patiño, Vicente
Medina-Escuela, Alfonso
Huertas, César S.
Calderón, Enrique J.
Eritja Casadellà, Ramón
Lechuga, Laura M.
author_role author
author2 Aviñó, Anna
Friaza Patiño, Vicente
Medina-Escuela, Alfonso
Huertas, César S.
Calderón, Enrique J.
Eritja Casadellà, Ramón
Lechuga, Laura M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat de Catalunya
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pneumocystis jirovecii
Surface plasmon resonance
Optical biosensor
Clinical diagnosis
Triplex
DNA capture
topic Pneumocystis jirovecii
Surface plasmon resonance
Optical biosensor
Clinical diagnosis
Triplex
DNA capture
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215702
url http://hdl.handle.net/10261/215702
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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https://doi.org/10.3390/nano10061246
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic BiosensorCalvo-Lozano, OlallaAviñó, AnnaFriaza Patiño, VicenteMedina-Escuela, AlfonsoHuertas, César S.Calderón, Enrique J.Eritja Casadellà, RamónLechuga, Laura M.Pneumocystis jiroveciiSurface plasmon resonanceOptical biosensorClinical diagnosisTriplexDNA capture© 2020 by the authors.Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, the standard diagnostic method employed for its diagnosis involves mainly PCR-based techniques, which besides being highly specific and sensitive, require specialized personnel and equipment and are time-consuming. Our aim is to demonstrate an optical biosensor methodology based on surface plasmon resonance to perform such diagnostics in an efficient and decentralized scheme. The biosensor methodology employs poly-purine reverse-Hoogsteen hairpin probes for the detection of the mitochondrial large subunit ribosomal RNA (mtLSU rRNA) gene, related to P. jirovecii detection. The biosensor device performs a real-time and label-free identification of the mtLSU rRNA gene with excellent selectivity and reproducibility, achieving limits of detection of around 2.11 nM. A preliminary evaluation of clinical samples showed rapid, label-free and specific identification of P. jirovecii in human lung fluids such as bronchoalveolar lavages or nasopharyngeal aspirates. These results offer a door for the future deployment of a sensitive diagnostic tool for fast, direct and selective detection of Pneumocystis pneumonia disease.The ICN2 is funded by the CERCA program/Generalitat de Catalunya. The ICN2 was supported by the Severo Ochoa Centres of Excellence program funded by the Spanish Research Agency (AEI, grant no. SEV-2017-0706). The ICN2 and O.C.L. acknowledge the economic support of the Spanish MINECO, the Spanish Research Agency and the European Social Fund (ESF) for the grant for predoctoral contracts in the Subprograma Estatal de Formación del Programa Estatal de Promoción del Talento y su Empleabilidad (ref. BES-2017-080527) linked to the TEC 2016-78515-R project PREDICT. Dr C.S.H. is a recipient of an RMIT Vice Chancellor’s Postdoctoral Fellowship. This study was supported, in part, by the Red Iberoamericana sobre Pneumocystosis in the framework of The Ibero-American Programme for Science, Technology and Development (grant CYTED 212RT0450). Financial support was also provided by the Spanish Ministerio de Ciencia e Innovación (MICINN) (Projects CTQ2017-84415-R).Peer reviewedMultidisciplinary Digital Publishing InstituteGeneralitat de CatalunyaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215702reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SEV-2017-0706SEV-2017-0706/AEI/10.13039/501100011033info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/TEC2016-78515-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84415-RCTQ2017-84415-R/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84415-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/TEC2016-78515-Rhttps://doi.org/10.3390/nano10061246Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2157022026-05-22T06:33:51Z
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