Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae

[EN] Background: Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurit...

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Autores: Gómez García, Manuel, Argüello Rodríguez, Héctor, Pérez Pérez, Lucía, Vega, Clara, Puente Fernández, Héctor, Mencía Ares, Óscar, Rubio Nistal, Pedro Miguel, Carvajal Urueña, Ana María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/17881
Acceso en línea:https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-021-00244-9
https://hdl.handle.net/10612/17881
Access Level:acceso abierto
Palabra clave:Sanidad animal
Swine dysentery
Disinfection
Cleaning
Internal biosecurity
3109.05 Microbiología
id ES_0147891625456daddea02cf8dfd36422
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spelling Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriaeGómez García, ManuelArgüello Rodríguez, HéctorPérez Pérez, LucíaVega, ClaraPuente Fernández, HéctorMencía Ares, ÓscarRubio Nistal, Pedro MiguelCarvajal Urueña, Ana MaríaSanidad animalSwine dysenteryDisinfectionCleaningInternal biosecurity3109.05 Microbiología[EN] Background: Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurity with cleaning and disinfection as crucial tools to avoid the pathogen transmission. This study evaluates the in-vitro efficacy of an array of commercial disinfectants against a collection of B. hyodysenteriae isolates using broth tests. The efficacy of cleaning and disinfection protocols was also evaluated on two farms with endemic SD using surface swabs collected in emptied pens before and after cleaning and disinfection procedures, using both real-time PCR and bacterial microbiological culture. Results: Most of the commercial disinfectants evaluated were effective against all B. hyodysenteriae isolates tested, with a reduction of more than 5.00 log10 CFU/mL (bactericidal efficacy of 99.999%). However, some isolates exhibited reduced susceptibility to Virkon-S and Limoseptic disinfectants. The evaluation of cleaning and disinfection protocols on farms with SD outbreaks showed that approximately half the pens tested (n = 25) were positive by real-time PCR after pigs removal (mean B. hyodysenteriae counts 5.72 ± 1.04 log10 CFU/mL) while almost 20% of the pens remained positive after cleaning (n = 7) and disinfection (n = 5) procedures although with significantly lower, mean estimates (4.31 ± 0.43 log10 CFU/mL and 4.01 ± 0.55 log10 CFU/mL, respectively). Conclusions: These results show the efficacy of disinfectants against B. hyodysenteriae but also stress the need to implement adequately the cleaning and disinfection protocols on pig farms and review and revise their efficiency periodically.SIThis study was supported by the Ministerio de Ciencia e Innovación (PID2019-110662RB-I00) and the Castilla y León Rural Development Pro‑ gramme co-fnanced by EAFRD (47/18/125/G02BioMed CentralSanidad AnimalFacultad de Veterinaria2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-021-00244-9https://hdl.handle.net/10612/17881reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/ AEI/ Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/ PID2019-110662RB-I00http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/178812026-06-24T12:43:27Z
dc.title.none.fl_str_mv Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
title Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
spellingShingle Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
Gómez García, Manuel
Sanidad animal
Swine dysentery
Disinfection
Cleaning
Internal biosecurity
3109.05 Microbiología
title_short Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
title_full Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
title_fullStr Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
title_full_unstemmed Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
title_sort Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
dc.creator.none.fl_str_mv Gómez García, Manuel
Argüello Rodríguez, Héctor
Pérez Pérez, Lucía
Vega, Clara
Puente Fernández, Héctor
Mencía Ares, Óscar
Rubio Nistal, Pedro Miguel
Carvajal Urueña, Ana María
author Gómez García, Manuel
author_facet Gómez García, Manuel
Argüello Rodríguez, Héctor
Pérez Pérez, Lucía
Vega, Clara
Puente Fernández, Héctor
Mencía Ares, Óscar
Rubio Nistal, Pedro Miguel
Carvajal Urueña, Ana María
author_role author
author2 Argüello Rodríguez, Héctor
Pérez Pérez, Lucía
Vega, Clara
Puente Fernández, Héctor
Mencía Ares, Óscar
Rubio Nistal, Pedro Miguel
Carvajal Urueña, Ana María
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Swine dysentery
Disinfection
Cleaning
Internal biosecurity
3109.05 Microbiología
topic Sanidad animal
Swine dysentery
Disinfection
Cleaning
Internal biosecurity
3109.05 Microbiología
description [EN] Background: Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurity with cleaning and disinfection as crucial tools to avoid the pathogen transmission. This study evaluates the in-vitro efficacy of an array of commercial disinfectants against a collection of B. hyodysenteriae isolates using broth tests. The efficacy of cleaning and disinfection protocols was also evaluated on two farms with endemic SD using surface swabs collected in emptied pens before and after cleaning and disinfection procedures, using both real-time PCR and bacterial microbiological culture. Results: Most of the commercial disinfectants evaluated were effective against all B. hyodysenteriae isolates tested, with a reduction of more than 5.00 log10 CFU/mL (bactericidal efficacy of 99.999%). However, some isolates exhibited reduced susceptibility to Virkon-S and Limoseptic disinfectants. The evaluation of cleaning and disinfection protocols on farms with SD outbreaks showed that approximately half the pens tested (n = 25) were positive by real-time PCR after pigs removal (mean B. hyodysenteriae counts 5.72 ± 1.04 log10 CFU/mL) while almost 20% of the pens remained positive after cleaning (n = 7) and disinfection (n = 5) procedures although with significantly lower, mean estimates (4.31 ± 0.43 log10 CFU/mL and 4.01 ± 0.55 log10 CFU/mL, respectively). Conclusions: These results show the efficacy of disinfectants against B. hyodysenteriae but also stress the need to implement adequately the cleaning and disinfection protocols on pig farms and review and revise their efficiency periodically.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-021-00244-9
https://hdl.handle.net/10612/17881
url https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-021-00244-9
https://hdl.handle.net/10612/17881
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ AEI/ Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/ PID2019-110662RB-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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