Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene

Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by in...

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Detalhes bibliográficos
Autores: Gil-Gálvez, Alejandro, Carbonell-Corvillo, Pilar, Paradas, Carmen, Miranda-Vizuete, Antonio
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/238738
Acesso em linha:http://hdl.handle.net/10261/238738
Access Level:acceso abierto
Palavra-chave:Caenorhabditis elegans
Mutation
MYH7
Myosin
Overexpression
UNC-54
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spelling Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 geneGil-Gálvez, AlejandroCarbonell-Corvillo, PilarParadas, CarmenMiranda-Vizuete, AntonioCaenorhabditis elegansMutationMYH7MyosinOverexpressionUNC-54Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations into the unc-54 gene, the worm MYH7 ortholog. We report here that the C. elegans model is not appropriate for such studies if they involve expression of the UNC-54 protein (wild-type or fused to green fluorescent protein) above endogenous levels.C Paradas was supported by grants from the Health Institute Carlos III and FEDER (FIS PI16-01843) and the Consejería de Salud, Junta de Andalucía (PI-0085-2016). A Miranda-Vizuete was supported by the Spanish Ministry of Economy and Competitiveness (BFU2015-64408-P). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.Future MedicineInstituto de Salud Carlos IIIEuropean CommissionJunta de AndalucíaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_545bPublisher's versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/contributionToPeriodicalhttp://hdl.handle.net/10261/238738reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64408-Phttp://dx.doi.org/10.2144/btn-2020-0012Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2387382026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
title Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
spellingShingle Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
Gil-Gálvez, Alejandro
Caenorhabditis elegans
Mutation
MYH7
Myosin
Overexpression
UNC-54
title_short Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
title_full Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
title_fullStr Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
title_full_unstemmed Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
title_sort Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene
dc.creator.none.fl_str_mv Gil-Gálvez, Alejandro
Carbonell-Corvillo, Pilar
Paradas, Carmen
Miranda-Vizuete, Antonio
author Gil-Gálvez, Alejandro
author_facet Gil-Gálvez, Alejandro
Carbonell-Corvillo, Pilar
Paradas, Carmen
Miranda-Vizuete, Antonio
author_role author
author2 Carbonell-Corvillo, Pilar
Paradas, Carmen
Miranda-Vizuete, Antonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Junta de Andalucía
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Caenorhabditis elegans
Mutation
MYH7
Myosin
Overexpression
UNC-54
topic Caenorhabditis elegans
Mutation
MYH7
Myosin
Overexpression
UNC-54
description Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations into the unc-54 gene, the worm MYH7 ortholog. We report here that the C. elegans model is not appropriate for such studies if they involve expression of the UNC-54 protein (wild-type or fused to green fluorescent protein) above endogenous levels.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_545b
Publisher's version
info:eu-repo/semantics/publishedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/contributionToPeriodical
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/238738
url http://hdl.handle.net/10261/238738
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64408-P
http://dx.doi.org/10.2144/btn-2020-0012
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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