Circadian expression of adiponectin and its receptors in human adipose tissue

Adiponectin is one of the most clinically relevant cytokines associated with obesity. However, circadian rhythmicity of adiponectin in human adipose tissue (AT) has not been analyzed. To assess whether the mRNA levels of adiponectin and its receptors (ADIPOR1 and ADIPOR2) might show daily circadian...

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Authors: Gomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934, Gomez-Santos, C. (C.)|||/items/5d0e7a5c-c7da-4eec-922a-641a3f7ebf69, Madrid, J.A. (J.A.)|||/items/13b10d86-d981-464e-8eaf-33be60908efd, Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131, Ordovas, J.M. (Jose Maria)|||/items/47377f9c-3ded-4da9-9b70-5425df93cfcc, Garaulet, M. (M.)|||/items/fba79804-a1f9-4565-b31f-6c94902e89e6
Format: article
Publication Date:2010
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/28189
Online Access:https://hdl.handle.net/10171/28189
Access Level:Open access
Keyword:Adipose tissue
Circadian rhythm
Receptors
Adiponectin
Obesity, morbid
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spelling Circadian expression of adiponectin and its receptors in human adipose tissueGomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934Gomez-Santos, C. (C.)|||/items/5d0e7a5c-c7da-4eec-922a-641a3f7ebf69Madrid, J.A. (J.A.)|||/items/13b10d86-d981-464e-8eaf-33be60908efdMilagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebCampión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cMartinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Ordovas, J.M. (Jose Maria)|||/items/47377f9c-3ded-4da9-9b70-5425df93cfccGaraulet, M. (M.)|||/items/fba79804-a1f9-4565-b31f-6c94902e89e6Adipose tissueCircadian rhythmReceptorsAdiponectinObesity, morbidAdiponectin is one of the most clinically relevant cytokines associated with obesity. However, circadian rhythmicity of adiponectin in human adipose tissue (AT) has not been analyzed. To assess whether the mRNA levels of adiponectin and its receptors (ADIPOR1 and ADIPOR2) might show daily circadian rhythms in visceral and sc fat explants obtained from morbid obese women, visceral and sc abdominal AT biopsies (n = 6) were obtained from morbidly obese women (body mass index >or=40 kg/m(2)). Anthropometric variables were measured and fasting plasma glucose, lipid, and lipoprotein concentrations were analyzed. To investigate rhythmic expression pattern, AT explants were cultured during 24 h, and gene expression was analyzed at the following times: 0800, 1400, 2000, and 0200 h, using quantitative real-time PCR. All genes investigated showed a circadian rhythmicity and oscillated accurately and independently of the suprachiasmatic nucleus in both AT explants (P < 0.05). Adiponectin gene expression fluctuated in the same phase as its receptors. Correlation analyses between the genetic circadian oscillation and components of the metabolic syndrome revealed that adiposity and abdominal obesity correlated with a decrease in adiponectin and adiponectin receptors ADIPOR1 and ADIPOR2 amplitude (P < 0.05). Visceral fat showed a trend toward a phase delay and dampening of the mRNA amplitude of adiponectin as compared with sc fat. The mRNA expression of adiponectin and its receptors showed 24-h rhythmicity in human AT from morbidly obese patients.Endocrine SocietyDadun. Depósito Académico Digital Universidad de Navarra20132013-03-2820102010-01-0120102010-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/28189reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/281892026-06-21T12:47:57Z
dc.title.none.fl_str_mv Circadian expression of adiponectin and its receptors in human adipose tissue
title Circadian expression of adiponectin and its receptors in human adipose tissue
spellingShingle Circadian expression of adiponectin and its receptors in human adipose tissue
Gomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934
Adipose tissue
Circadian rhythm
Receptors
Adiponectin
Obesity, morbid
title_short Circadian expression of adiponectin and its receptors in human adipose tissue
title_full Circadian expression of adiponectin and its receptors in human adipose tissue
title_fullStr Circadian expression of adiponectin and its receptors in human adipose tissue
title_full_unstemmed Circadian expression of adiponectin and its receptors in human adipose tissue
title_sort Circadian expression of adiponectin and its receptors in human adipose tissue
dc.creator.none.fl_str_mv Gomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934
Gomez-Santos, C. (C.)|||/items/5d0e7a5c-c7da-4eec-922a-641a3f7ebf69
Madrid, J.A. (J.A.)|||/items/13b10d86-d981-464e-8eaf-33be60908efd
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Ordovas, J.M. (Jose Maria)|||/items/47377f9c-3ded-4da9-9b70-5425df93cfcc
Garaulet, M. (M.)|||/items/fba79804-a1f9-4565-b31f-6c94902e89e6
author Gomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934
author_facet Gomez-Abellan, P. (P.)|||/items/ee64026e-c3ac-4ae9-9b93-e8c812df5934
Gomez-Santos, C. (C.)|||/items/5d0e7a5c-c7da-4eec-922a-641a3f7ebf69
Madrid, J.A. (J.A.)|||/items/13b10d86-d981-464e-8eaf-33be60908efd
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Ordovas, J.M. (Jose Maria)|||/items/47377f9c-3ded-4da9-9b70-5425df93cfcc
Garaulet, M. (M.)|||/items/fba79804-a1f9-4565-b31f-6c94902e89e6
author_role author
author2 Gomez-Santos, C. (C.)|||/items/5d0e7a5c-c7da-4eec-922a-641a3f7ebf69
Madrid, J.A. (J.A.)|||/items/13b10d86-d981-464e-8eaf-33be60908efd
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Ordovas, J.M. (Jose Maria)|||/items/47377f9c-3ded-4da9-9b70-5425df93cfcc
Garaulet, M. (M.)|||/items/fba79804-a1f9-4565-b31f-6c94902e89e6
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Adipose tissue
Circadian rhythm
Receptors
Adiponectin
Obesity, morbid
topic Adipose tissue
Circadian rhythm
Receptors
Adiponectin
Obesity, morbid
description Adiponectin is one of the most clinically relevant cytokines associated with obesity. However, circadian rhythmicity of adiponectin in human adipose tissue (AT) has not been analyzed. To assess whether the mRNA levels of adiponectin and its receptors (ADIPOR1 and ADIPOR2) might show daily circadian rhythms in visceral and sc fat explants obtained from morbid obese women, visceral and sc abdominal AT biopsies (n = 6) were obtained from morbidly obese women (body mass index >or=40 kg/m(2)). Anthropometric variables were measured and fasting plasma glucose, lipid, and lipoprotein concentrations were analyzed. To investigate rhythmic expression pattern, AT explants were cultured during 24 h, and gene expression was analyzed at the following times: 0800, 1400, 2000, and 0200 h, using quantitative real-time PCR. All genes investigated showed a circadian rhythmicity and oscillated accurately and independently of the suprachiasmatic nucleus in both AT explants (P < 0.05). Adiponectin gene expression fluctuated in the same phase as its receptors. Correlation analyses between the genetic circadian oscillation and components of the metabolic syndrome revealed that adiposity and abdominal obesity correlated with a decrease in adiponectin and adiponectin receptors ADIPOR1 and ADIPOR2 amplitude (P < 0.05). Visceral fat showed a trend toward a phase delay and dampening of the mRNA amplitude of adiponectin as compared with sc fat. The mRNA expression of adiponectin and its receptors showed 24-h rhythmicity in human AT from morbidly obese patients.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01
2010
2010-01-01
2013
2013-03-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/28189
url https://hdl.handle.net/10171/28189
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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