Combining phylogenetic footprinting with motif models incorporating intra-motif dependencies

[ Background] Transcriptional gene regulation is a fundamental process in nature, and the experimental and computational investigation of DNA binding motifs and their binding sites is a prerequisite for elucidating this process. Approaches for de-novo motif discovery can be subdivided in phylogeneti...

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Detalhes bibliográficos
Autores: Nettling, Martin, Treutler, Hendrik, Cerquides, Jesús, Grosse, Ivo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/145968
Acesso em linha:http://hdl.handle.net/10261/145968
Access Level:acceso abierto
Palavra-chave:ChIP-seq
Phylogenetic footprinting
Evolution
Transcription factor binding sites
Gene regulation
Descrição
Resumo:[ Background] Transcriptional gene regulation is a fundamental process in nature, and the experimental and computational investigation of DNA binding motifs and their binding sites is a prerequisite for elucidating this process. Approaches for de-novo motif discovery can be subdivided in phylogenetic footprinting that takes into account phylogenetic dependencies in aligned sequences of more than one species and non-phylogenetic approaches based on sequences from only one species that typically take into account intra-motif dependencies. It has been shown that modeling (i) phylogenetic dependencies as well as (ii) intra-motif dependencies separately improves de-novo motif discovery, but there is no approach capable of modeling both (i) and (ii) simultaneously.