Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis

11 páginas, 5 figuras.

Detalles Bibliográficos
Autores: Fernández Fernández, Miguel, Royo González, Marcos, Arteche-Villasol, Noive, Ferreras, Mª del Carmen, Pérez Pérez, Valentín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/286986
Acceso en línea:http://hdl.handle.net/10261/286986
Access Level:acceso abierto
Palabra clave:Paratuberculosis
Vaccination
Ruminants
Mycobacterium avium subspecies paratuberculosis
Interferon-gamma
Immune response
Tuberculosis
Antigens
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spelling Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosisFernández Fernández, MiguelRoyo González, MarcosArteche-Villasol, NoiveFerreras, Mª del CarmenPérez Pérez, ValentínParatuberculosisVaccinationRuminantsMycobacterium avium subspecies paratuberculosisInterferon-gammaImmune responseTuberculosisAntigens11 páginas, 5 figuras.Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of Mycobacterium bovis (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of Mycobacterium avium subspecies paratuberculosis (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new Mycobacterium bovis antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats.This research was funded by Ministry of Economy, Industry and Competitiveness through the research project with reference: AGL2012-39818-C02-01 titled “Development of pathogenesis and immunization models in paratuberculosis”. Marcos Royo and Noive Arteche-Villasol were recipients of a predoctoral contract for training of doctors by the Ministry of Economy, Industry and Competitiveness.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Arteche Villasol, Noive [0000-0001-5793-9578]Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]Pérez Pérez, Valentín [0000-0003-0075-1587]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320222023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/286986reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2012-39818-C02-01http://dx.doi.org/10.3390/vaccines10101709Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2869862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
spellingShingle Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
Fernández Fernández, Miguel
Paratuberculosis
Vaccination
Ruminants
Mycobacterium avium subspecies paratuberculosis
Interferon-gamma
Immune response
Tuberculosis
Antigens
title_short Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title_full Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title_fullStr Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title_full_unstemmed Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
title_sort Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
dc.creator.none.fl_str_mv Fernández Fernández, Miguel
Royo González, Marcos
Arteche-Villasol, Noive
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
author Fernández Fernández, Miguel
author_facet Fernández Fernández, Miguel
Royo González, Marcos
Arteche-Villasol, Noive
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
author_role author
author2 Royo González, Marcos
Arteche-Villasol, Noive
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Arteche Villasol, Noive [0000-0001-5793-9578]
Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Paratuberculosis
Vaccination
Ruminants
Mycobacterium avium subspecies paratuberculosis
Interferon-gamma
Immune response
Tuberculosis
Antigens
topic Paratuberculosis
Vaccination
Ruminants
Mycobacterium avium subspecies paratuberculosis
Interferon-gamma
Immune response
Tuberculosis
Antigens
description 11 páginas, 5 figuras.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/286986
url http://hdl.handle.net/10261/286986
dc.language.none.fl_str_mv Inglés
Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2012-39818-C02-01
http://dx.doi.org/10.3390/vaccines10101709
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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