Primary sequence and 3D structure prediction of the plant toxin stenodactylin

Producción Científica

Detalhes bibliográficos
Autores: Iglesias Álvarez, María del Rosario, Polito, Letizia, Bortolotti, Massimo, Pedrazzi, Manuela, Citores González, Lucía, Ferreras Rodríguez, José Miguel, Bolognesi, Andrea
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/58968
Acesso em linha:https://doi.org/10.3390/toxins12090538
https://uvadoc.uva.es/handle/10324/58968
Access Level:acceso abierto
Palavra-chave:Proteins - Synthesis
Proteínas
Ribosomes - Structure
3D structure
Plant toxin
Primary sequence
Ribosome-inactivating protein
Stenodactylin
3209 Farmacología
3214 Toxicología
id ES_0072fc64fd110e751d8552bcfd98a762
oai_identifier_str oai:uvadoc.uva.es:10324/58968
network_acronym_str ES
network_name_str España
repository_id_str
spelling Primary sequence and 3D structure prediction of the plant toxin stenodactylinIglesias Álvarez, María del RosarioPolito, LetiziaBortolotti, MassimoPedrazzi, ManuelaCitores González, LucíaFerreras Rodríguez, José MiguelBolognesi, AndreaProteins - SynthesisProteínasRibosomes - Structure3D structurePlant toxinPrimary sequenceRibosome-inactivating proteinStenodactylin3209 Farmacología3214 ToxicologíaProducción CientíficaStenodactylin is one of the most potent type 2 ribosome-inactivating proteins (RIPs); its high toxicity has been demonstrated in several models both in vitro and in vivo. Due to its peculiarities, stenodactylin could have several medical and biotechnological applications in neuroscience and cancer treatment. In this work, we report the complete amino acid sequence of stenodactylin and 3D structure prediction. The comparison between the primary sequence of stenodactylin and other RIPs allowed us to identify homologies/differences and the amino acids involved in RIP toxic activity. Stenodactylin RNA was isolated from plant caudex, reverse transcribed through PCR and the cDNA was amplificated and cloned into a plasmid vector and further analyzed by sequencing. Nucleotide sequence analysis showed that stenodactylin A and B chains contain 251 and 258 amino acids, respectively. The key amino acids of the active site described for ricin and most other RIPs are also conserved in the stenodactylin A chain. Stenodactylin amino acid sequence shows a high identity degree with volkensin (81.7% for A chain, 90.3% for B chain), whilst when compared with other type 2 RIPs the identity degree ranges from 27.7 to 33.0% for the A chain and from 42.1 to 47.7% for the B chain.Universidad de Bolonia y Pallotti Legacies for Cancer Research; Fundación CARISBO - (Project 2019.0539)Junta de Castilla y León - (Project VA033G19)MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3390/toxins12090538https://uvadoc.uva.es/handle/10324/58968reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.mdpi.com/2072-6651/12/9/538info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/589682026-06-13T12:44:47Z
dc.title.none.fl_str_mv Primary sequence and 3D structure prediction of the plant toxin stenodactylin
title Primary sequence and 3D structure prediction of the plant toxin stenodactylin
spellingShingle Primary sequence and 3D structure prediction of the plant toxin stenodactylin
Iglesias Álvarez, María del Rosario
Proteins - Synthesis
Proteínas
Ribosomes - Structure
3D structure
Plant toxin
Primary sequence
Ribosome-inactivating protein
Stenodactylin
3209 Farmacología
3214 Toxicología
title_short Primary sequence and 3D structure prediction of the plant toxin stenodactylin
title_full Primary sequence and 3D structure prediction of the plant toxin stenodactylin
title_fullStr Primary sequence and 3D structure prediction of the plant toxin stenodactylin
title_full_unstemmed Primary sequence and 3D structure prediction of the plant toxin stenodactylin
title_sort Primary sequence and 3D structure prediction of the plant toxin stenodactylin
dc.creator.none.fl_str_mv Iglesias Álvarez, María del Rosario
Polito, Letizia
Bortolotti, Massimo
Pedrazzi, Manuela
Citores González, Lucía
Ferreras Rodríguez, José Miguel
Bolognesi, Andrea
author Iglesias Álvarez, María del Rosario
author_facet Iglesias Álvarez, María del Rosario
Polito, Letizia
Bortolotti, Massimo
Pedrazzi, Manuela
Citores González, Lucía
Ferreras Rodríguez, José Miguel
Bolognesi, Andrea
author_role author
author2 Polito, Letizia
Bortolotti, Massimo
Pedrazzi, Manuela
Citores González, Lucía
Ferreras Rodríguez, José Miguel
Bolognesi, Andrea
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteins - Synthesis
Proteínas
Ribosomes - Structure
3D structure
Plant toxin
Primary sequence
Ribosome-inactivating protein
Stenodactylin
3209 Farmacología
3214 Toxicología
topic Proteins - Synthesis
Proteínas
Ribosomes - Structure
3D structure
Plant toxin
Primary sequence
Ribosome-inactivating protein
Stenodactylin
3209 Farmacología
3214 Toxicología
description Producción Científica
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/toxins12090538
https://uvadoc.uva.es/handle/10324/58968
url https://doi.org/10.3390/toxins12090538
https://uvadoc.uva.es/handle/10324/58968
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2072-6651/12/9/538
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402500385210368
score 15,300719

Registos relacionados