Isolation and characterization of nudC from mouse macrophages, a gene implicated in the inflammatory response through the regulation of PAF-AH(I) activity

We report the characterization of a cDNA induced in mouse macrophages that encodes a 332-amino acid protein with extensive sequence identity with members of the mammalian nudC-like genes. The interaction between mNUDC and the regulatory β subunit of platelet activating factor acetylhydrolase I (PAF-...

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Detalles Bibliográficos
Autores: Riera, José, Rodríguez, René, Carcedo, María Teresa, Campa, Víctor M., Ramos, Sofía, Lazo, Pedro S.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414193
Acceso en línea:http://hdl.handle.net/10261/414193
Access Level:acceso abierto
Palabra clave:GST
Glutathione-S-transferase
LIS-1 lisoencephaly gene 1
NudC
Nuclear distribution clone C
NudF nuclear distribution clone F
PAF platelet activating factor
PAF-AH(I)
Platelet activating factor acetylhydrolase type I
Monocytes/macrophages
Inflammation
Platelet activating factor acetylhydrolase nudC
Descripción
Sumario:We report the characterization of a cDNA induced in mouse macrophages that encodes a 332-amino acid protein with extensive sequence identity with members of the mammalian nudC-like genes. The interaction between mNUDC and the regulatory β subunit of platelet activating factor acetylhydrolase I (PAF-AH(I)) shown in this article indicates a new function of NUDC. Thus, we show that NUDC increases the catalytic activity of PAF-AH(I) and that this regulatory activity is located in the carboxyl terminal half of the protein which is highly conserved. This suggests a novel function for mammalian nudC-like genes as anti-inflammatory proteins.