Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:272490 |
| Acceso en línea: | https://ddd.uab.cat/record/272490 https://dx.doi.org/urn:doi:10.3389/fimmu.2022.1020227 |
| Access Level: | acceso abierto |
| Palabra clave: | CTL NKT NK PRRSV-1 Transplacental infection |
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Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infectionLi, YanliDíaz, Ivan|||0000-0002-8090-1073Martin-Valls, Gerard|||0000-0003-3302-0692Beyersdorf, NiklasMateu de Antonio, Enrique María|||0000-0003-2715-6032CTLNKTNKPRRSV-1Transplacental infectionPorcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and cause transplacental infection. The immune effector mechanisms involved are largely unknown. The present study investigated the role of cytotoxic lymphocytes, including cytotoxic T cells (CTL), NKT, and NK cells, from blood in preventing PRRSV-1 transplacental infection in vaccinated primiparous sows (two doses vaccinated). Sows from a PRRSV-1 unstable farm were bled just before the last month of gestation (critical period for transplacental infection), then followed to determine whether sows delivered PRRSV-1-infected (n=8) or healthy (n=10) piglets. After that, functions of CTL, NKT, and NK cells in the two groups of sows were compared. No difference was found through cell surface staining. But upon in vitro re-stimulation with the circulating field virus, sows that delivered healthy piglets displayed a higher frequency of virus-specific CD107a + IFN-γ-producing T cells, which accumulated in the CD4 + compartment including CD4 single-positive (CD4 SP) and CD4/CD8α double-positive (CD4/CD8α DP) subsets. The same group of sows also harbored a higher proportion of CD107a + TNF-α-producing T cells that predominantly accumulated in CD4/CD8α double-negative (CD4/CD8α DN) subset. Consistently, CD4 SP and CD4/CD8α DN T cells from sows delivering healthy piglets had a higher virus-specific proliferative response. Additionally, in sows that delivered PRRSV-1-infected piglets, a positive correlation of virus-specific IFN-γ response with average Ct values of umbilical cords of newborn piglets per litter was observed. Our data strongly suggest that CTL responses correlate with protection against PRRSV-1 transplacental infection, being executed by CD4 T cells (IFN-γ related) and/or CD4/CD8α DN T cells (TNF-α related). 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/272490https://dx.doi.org/urn:doi:10.3389/fimmu.2022.1020227reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2724902026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| title |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| spellingShingle |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection Li, Yanli CTL NKT NK PRRSV-1 Transplacental infection |
| title_short |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| title_full |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| title_fullStr |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| title_full_unstemmed |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| title_sort |
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection |
| dc.creator.none.fl_str_mv |
Li, Yanli Díaz, Ivan|||0000-0002-8090-1073 Martin-Valls, Gerard|||0000-0003-3302-0692 Beyersdorf, Niklas Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author |
Li, Yanli |
| author_facet |
Li, Yanli Díaz, Ivan|||0000-0002-8090-1073 Martin-Valls, Gerard|||0000-0003-3302-0692 Beyersdorf, Niklas Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author_role |
author |
| author2 |
Díaz, Ivan|||0000-0002-8090-1073 Martin-Valls, Gerard|||0000-0003-3302-0692 Beyersdorf, Niklas Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
CTL NKT NK PRRSV-1 Transplacental infection |
| topic |
CTL NKT NK PRRSV-1 Transplacental infection |
| description |
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and cause transplacental infection. The immune effector mechanisms involved are largely unknown. The present study investigated the role of cytotoxic lymphocytes, including cytotoxic T cells (CTL), NKT, and NK cells, from blood in preventing PRRSV-1 transplacental infection in vaccinated primiparous sows (two doses vaccinated). Sows from a PRRSV-1 unstable farm were bled just before the last month of gestation (critical period for transplacental infection), then followed to determine whether sows delivered PRRSV-1-infected (n=8) or healthy (n=10) piglets. After that, functions of CTL, NKT, and NK cells in the two groups of sows were compared. No difference was found through cell surface staining. But upon in vitro re-stimulation with the circulating field virus, sows that delivered healthy piglets displayed a higher frequency of virus-specific CD107a + IFN-γ-producing T cells, which accumulated in the CD4 + compartment including CD4 single-positive (CD4 SP) and CD4/CD8α double-positive (CD4/CD8α DP) subsets. The same group of sows also harbored a higher proportion of CD107a + TNF-α-producing T cells that predominantly accumulated in CD4/CD8α double-negative (CD4/CD8α DN) subset. Consistently, CD4 SP and CD4/CD8α DN T cells from sows delivering healthy piglets had a higher virus-specific proliferative response. Additionally, in sows that delivered PRRSV-1-infected piglets, a positive correlation of virus-specific IFN-γ response with average Ct values of umbilical cords of newborn piglets per litter was observed. Our data strongly suggest that CTL responses correlate with protection against PRRSV-1 transplacental infection, being executed by CD4 T cells (IFN-γ related) and/or CD4/CD8α DN T cells (TNF-α related). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/272490 https://dx.doi.org/urn:doi:10.3389/fimmu.2022.1020227 |
| url |
https://ddd.uab.cat/record/272490 https://dx.doi.org/urn:doi:10.3389/fimmu.2022.1020227 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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