One-Pot Peptide Ligation – Oxidative Cyclization Protocol for the Preparation of Short/Medium Size Disulfide Cyclopeptides
Native chemical ligation (NCL) employing the N-methylbenzimidazolinone (MeNbz) linker readily provided the linear precursor of a 16-mer peptide that is difficult to obtain by stepwise solid-phase peptide synthesis. NCL and the workup conditions were improved toward a protocol that allows for quantitat...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Ecuador |
| Institución: | Universidad Regional Amazónica |
| Repositorio: | Repositorio Universidad Regional Amazónica |
| OAI Identifier: | oai:repositorio.ikiam.edu.ec:RD_IKIAM/213 |
| Acceso en línea: | https://doi.org/10.1021/acs.orglett.8b01741 http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/213 |
| Access Level: | acceso abierto |
| Palabra clave: | Ligation–Oxidativ Cyclopeptides Disulfide |
| Sumario: | Native chemical ligation (NCL) employing the N-methylbenzimidazolinone (MeNbz) linker readily provided the linear precursor of a 16-mer peptide that is difficult to obtain by stepwise solid-phase peptide synthesis. NCL and the workup conditions were improved toward a protocol that allows for quantitative removal of the 4-hydroxymercapto- phenol additive and subsequent formation of the disulfide bridge in the NCL cocktail by oxidation in air, tolerated by the presence of tris(hydroxypropyl)phosphine. |
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