Enriquecimiento de ácidos grasos poliinsaturados por Rhodomonas salina (Cryptophyta) después de mutagénesis aleatoria

A protocol through mutagenesis with ethyl methane sulphonate and selection with herbicide Quizalofop was developed for Rhodomonas salina. Mutants showing resistance to Quizalofop had enriched lipid and PUFAs content. Two mutants were obtained with a significantly increased capacity for lipids (50-60...

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Detalles Bibliográficos
Autores: Guevara, Miguel, Gómez, Patricia, Acosta, Mercedes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Ecuador
Institución:Universidad Técnica de Manabi
Repositorio:Revista Aquatechnica
Idioma:español
OAI Identifier:oai:ojs2.revistas.utm.edu.ec:article/2679
Acceso en línea:https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/2679
Access Level:acceso abierto
Palabra clave:Microalgae
Random mutagenesis
Fatty acids
Genetic improvement
Microalgas
Mutagénesis aleatoria
Ácidos grasos
Mejoramiento genético
Descripción
Sumario:A protocol through mutagenesis with ethyl methane sulphonate and selection with herbicide Quizalofop was developed for Rhodomonas salina. Mutants showing resistance to Quizalofop had enriched lipid and PUFAs content. Two mutants were obtained with a significantly increased capacity for lipids (50-60% higher than WT) and PUFAs (50% higher that WT) accumulations, demonstrating the feasibility of genetically improving a species that doesn´t form discrete colonies in solid medium.