Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog

Poison frogs sequester chemical defenses from arthropod prey, although the details of how arthropod diversity contributes to variation in poison frog toxins remains unclear. We characterized skin alkaloid profiles in the Little Devil poison frog, Oophaga sylvatica (Dendrobatidae), across three popul...

Descripción completa

Detalles Bibliográficos
Autores: McGugan, Jenna R., Byrd, Gary D., Roland, Alexandre B., Caty, Stephanie N., Kabir, Nisha, Tapia, Elicio E., Trauger, Sunia A., Coloma, Luis A., O’Connell, Lauren A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Ecuador
Institución:Universidad Regional Amazónica
Repositorio:Repositorio Universidad Regional Amazónica
OAI Identifier:oai:repositorio.ikiam.edu.ec:RD_IKIAM/166
Acceso en línea:https://doi.org/10.1007/s10886-016-0715-x
http://dspace.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/166
Access Level:acceso abierto
Palabra clave:Poison frog
Alkaloid
Toxin
Ant
Mite
Mass spectrometry
Dendrobatidae
id EC_5fd13ff4eceabf7787f4464b69b430c3
oai_identifier_str oai:repositorio.ikiam.edu.ec:RD_IKIAM/166
network_acronym_str EC
network_name_str Ecuador
repository_id_str
spelling Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison FrogMcGugan, Jenna R.Byrd, Gary D.Roland, Alexandre B.Caty, Stephanie N.Kabir, NishaTapia, Elicio E.Trauger, Sunia A.Coloma, Luis A.O’Connell, Lauren A.Poison frogAlkaloidToxinAntMiteMass spectrometryDendrobatidaePoison frogs sequester chemical defenses from arthropod prey, although the details of how arthropod diversity contributes to variation in poison frog toxins remains unclear. We characterized skin alkaloid profiles in the Little Devil poison frog, Oophaga sylvatica (Dendrobatidae), across three populations in northwestern Ecuador. Using gas chromatography/mass spectrometry, we identified histrionicotoxins, 3,5- and 5,8-disubstituted indolizidines, decahydroquinolines, and lehmizidines as the primary alkaloid toxins in these O. sylvatica populations. Frog skin alkaloid composition varied along a geographical gradient following population distribution in a principal component analysis. We also characterized diversity in arthropods isolated from frog stomach contents and confirmed that O. sylvatica specialize on ants and mites. To test the hypothesis that poison frog toxin variability reflects species and chemical diversity in arthropod prey, we (1) used sequencing of cytochrome oxidase 1 to identify individual prey specimens, and (2) used liquid chromatography/mass spectrometry to chemically profile consumed ants and mites. We identified 45 ants and 9 mites in frog stomachs, including several undescribed species. We also showed that chemical profiles of consumed ants and mites cluster by frog population, suggesting different frog populations have access to chemically distinct prey. Finally, by comparing chemical profiles of frog skin and isolated prey items, we traced the arthropod source of four poison frog alkaloids, including 3,5- and 5,8-disubstituted indolizidines and a lehmizidine alkaloid. Together, the data show that toxin variability in O. sylvatica reflects chemical diversity in arthropod prey.Kluwer Academic/Plenum Publishers2019-06-06T16:40:54Z2019-06-06T16:40:54Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMcGugan, J. R., Byrd, G. D., Roland, A. B., Caty, S. N., Kabir, N., Tapia, E. E., ... O’Connell, L. A. (2016). Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog. Journal of Chemical Ecology, 42(6), 537–551. doi: 10.1007/s10886-016-0715-xhttps://doi.org/10.1007/s10886-016-0715-xhttp://dspace.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/166https://doi.org/10.1007/s10886-016-0715-xenPRODUCCIÓN CIENTÍFICA-ARTÍCULOS;A-IKIAM-000104Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de Américahttp://creativecommons.org/licenses/by-nc-nd/3.0/us/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Regional Amazónicainstname:Universidad Regional Amazónicainstacron:IKIAM2022-06-04T08:02:19Zoai:repositorio.ikiam.edu.ec:RD_IKIAM/166Institucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oaiEcuador...opendoar:02022-06-04T08:02:19falseInstitucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oai.Ecuador...opendoar:02022-06-04T08:02:19Repositorio Universidad Regional Amazónica - Universidad Regional Amazónicafalse
dc.title.none.fl_str_mv Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
title Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
spellingShingle Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
McGugan, Jenna R.
Poison frog
Alkaloid
Toxin
Ant
Mite
Mass spectrometry
Dendrobatidae
title_short Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
title_full Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
title_fullStr Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
title_full_unstemmed Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
title_sort Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog
dc.creator.none.fl_str_mv McGugan, Jenna R.
Byrd, Gary D.
Roland, Alexandre B.
Caty, Stephanie N.
Kabir, Nisha
Tapia, Elicio E.
Trauger, Sunia A.
Coloma, Luis A.
O’Connell, Lauren A.
author McGugan, Jenna R.
author_facet McGugan, Jenna R.
Byrd, Gary D.
Roland, Alexandre B.
Caty, Stephanie N.
Kabir, Nisha
Tapia, Elicio E.
Trauger, Sunia A.
Coloma, Luis A.
O’Connell, Lauren A.
author_role author
author2 Byrd, Gary D.
Roland, Alexandre B.
Caty, Stephanie N.
Kabir, Nisha
Tapia, Elicio E.
Trauger, Sunia A.
Coloma, Luis A.
O’Connell, Lauren A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Poison frog
Alkaloid
Toxin
Ant
Mite
Mass spectrometry
Dendrobatidae
topic Poison frog
Alkaloid
Toxin
Ant
Mite
Mass spectrometry
Dendrobatidae
description Poison frogs sequester chemical defenses from arthropod prey, although the details of how arthropod diversity contributes to variation in poison frog toxins remains unclear. We characterized skin alkaloid profiles in the Little Devil poison frog, Oophaga sylvatica (Dendrobatidae), across three populations in northwestern Ecuador. Using gas chromatography/mass spectrometry, we identified histrionicotoxins, 3,5- and 5,8-disubstituted indolizidines, decahydroquinolines, and lehmizidines as the primary alkaloid toxins in these O. sylvatica populations. Frog skin alkaloid composition varied along a geographical gradient following population distribution in a principal component analysis. We also characterized diversity in arthropods isolated from frog stomach contents and confirmed that O. sylvatica specialize on ants and mites. To test the hypothesis that poison frog toxin variability reflects species and chemical diversity in arthropod prey, we (1) used sequencing of cytochrome oxidase 1 to identify individual prey specimens, and (2) used liquid chromatography/mass spectrometry to chemically profile consumed ants and mites. We identified 45 ants and 9 mites in frog stomachs, including several undescribed species. We also showed that chemical profiles of consumed ants and mites cluster by frog population, suggesting different frog populations have access to chemically distinct prey. Finally, by comparing chemical profiles of frog skin and isolated prey items, we traced the arthropod source of four poison frog alkaloids, including 3,5- and 5,8-disubstituted indolizidines and a lehmizidine alkaloid. Together, the data show that toxin variability in O. sylvatica reflects chemical diversity in arthropod prey.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-06-06T16:40:54Z
2019-06-06T16:40:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv McGugan, J. R., Byrd, G. D., Roland, A. B., Caty, S. N., Kabir, N., Tapia, E. E., ... O’Connell, L. A. (2016). Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog. Journal of Chemical Ecology, 42(6), 537–551. doi: 10.1007/s10886-016-0715-x
https://doi.org/10.1007/s10886-016-0715-x
http://dspace.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/166
https://doi.org/10.1007/s10886-016-0715-x
identifier_str_mv McGugan, J. R., Byrd, G. D., Roland, A. B., Caty, S. N., Kabir, N., Tapia, E. E., ... O’Connell, L. A. (2016). Ant and Mite Diversity Drives Toxin Variation in the Little Devil Poison Frog. Journal of Chemical Ecology, 42(6), 537–551. doi: 10.1007/s10886-016-0715-x
url https://doi.org/10.1007/s10886-016-0715-x
http://dspace.ikiam.edu.ec:8080/jspui/handle/RD_IKIAM/166
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv PRODUCCIÓN CIENTÍFICA-ARTÍCULOS;A-IKIAM-000104
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Kluwer Academic/Plenum Publishers
publisher.none.fl_str_mv Kluwer Academic/Plenum Publishers
dc.source.none.fl_str_mv reponame:Repositorio Universidad Regional Amazónica
instname:Universidad Regional Amazónica
instacron:IKIAM
instname_str Universidad Regional Amazónica
instacron_str IKIAM
institution IKIAM
reponame_str Repositorio Universidad Regional Amazónica
collection Repositorio Universidad Regional Amazónica
repository.name.fl_str_mv Repositorio Universidad Regional Amazónica - Universidad Regional Amazónica
repository.mail.fl_str_mv .
_version_ 1868999609472253952
score 14.965132

Similares en LA Referencia