Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja

Brucellosis is an infectious disease caused by microorganisms of the genus Brucella that affect different species of mammals.  The bacteria cause abortion, epididymitis, vesiculitis, birth of weak calves, the decrease in milk production, and infertility in cows and bulls. The disease is a zoonosis a...

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Autores: Román Cárdenas, Franklin, Ramón-Contento, Pablo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Ecuador
Institución:Universidad Central del Ecuador
Repositorio:Revista Siembra
Idioma:español
OAI Identifier:oai:revistadigital.uce.edu.ec:article/2242
Acceso en línea:https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242
Access Level:acceso abierto
Palabra clave:Brucella spp.
aborto
zoonosis
PCR
diagnóstico molecular
abortion
molecular diagnosis
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spelling Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in LojaIdentificación molecular de Brucella abortus en nódulos linfáticos de bovinos faenados en LojaRomán Cárdenas, FranklinRamón-Contento, Pablo Brucella spp.abortozoonosisPCRdiagnóstico molecularBrucella spp.abortionzoonosisPCRmolecular diagnosisBrucellosis is an infectious disease caused by microorganisms of the genus Brucella that affect different species of mammals.  The bacteria cause abortion, epididymitis, vesiculitis, birth of weak calves, the decrease in milk production, and infertility in cows and bulls. The disease is a zoonosis and is considered a public health problem. The objective of this work was to identify Brucella abortus by the Polymerase Chain Reaction. It was possible through the amplification of a fragment of 223 bp of the BCSP31 gene region, as DNA source, obtained from lymph nodes of the region cervical, pulmonary, bronchial, retropharyngeal, and mediastinal. One hundred and fifteen samples from animals slaughtered at Loja city abattoir in 2015 were analyzed. In 14.8% (17) of the slaughtered animals, the test was positive; although the presence of the bacteria was observed more frequently in the cervical, bronchial and retropharyngeal regions. The age of the positive animals ranged between 4 and 6 years. With the present study, it was confirmed the presence of the bacterium Brucella abortus through the molecular PCR test obtained from lymph nodes of slaughtered animals in the Loja canton.La brucelosis es una enfermedad infecciosa causada por microorganismos del género Brucella que afectan a diferentes especies de mamíferos; la cual provoca aborto, epididimitis, vesiculitis, nacimiento de terneros débiles, merma en la producción de leche, infertilidad y subfertilidad en vacas y toros. La enfermedad por ser zoonótica se considera un problema de salud pública. El objetivo de este diagnóstico fue identificar Brucella abortus mediante la reacción en cadena de la polimerasa. Se logró amplificar un fragmento de 223 pb de la región del gen BCSP31 como fuente de ADN, obtenido a partir de muestras de nódulos linfáticos de la región cervical, pulmonar, bronquial, retrofaríngea y mediastínica. Se analizaron 115 animales faenados en un camal de Loja en el año 2015. La edad de los animales osciló entre 4 y 6 años. En el 14,8 % (17) de los animales faenados se registró positividad a la prueba; aunque la presencia de la bacteria se observó con mayor frecuencia en las regiones cervical, bronquial y retrofaríngea. Con el presente estudio mediante la prueba molecular de PCR se demuestra la presencia de la bacteria Brucella abortus al encontrarla en nódulos linfáticos de animales faenados en el cantón Loja.Universidad Central del Ecuador2021-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/ziptext/xmlhttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/224210.29166/siembra.v8i1.2242Siembra; Vol. 8 No. 1 (2021): SIEMBRA; e2242Siembra; Vol. 8 Núm. 1 (2021): SIEMBRA; e22422477-88501390-892810.29166/siembra.v8i1reponame:Revista Siembrainstname:Universidad Central del Ecuadorinstacron:UCEspahttps://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3591https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/4039https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3610https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3611Derechos de autor 2021 Franklin Román Cárdenas, Pablo Ramón-Contentohttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccess2021-01-04T22:50:05Zoai:revistadigital.uce.edu.ec:article/2242Portal de revistashttps://revistadigital.uce.edu.ec/Universidad públicahttps://uce.edu.ec/**Ecuador*2477-88501390-8928opendoar:*2021-01-04T22:50:05Revista Siembra - Universidad Central del Ecuadorfalse
dc.title.none.fl_str_mv Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
Identificación molecular de Brucella abortus en nódulos linfáticos de bovinos faenados en Loja
title Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
spellingShingle Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
Román Cárdenas, Franklin
Brucella spp.
aborto
zoonosis
PCR
diagnóstico molecular
Brucella spp.
abortion
zoonosis
PCR
molecular diagnosis
title_short Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
title_full Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
title_fullStr Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
title_full_unstemmed Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
title_sort Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
dc.creator.none.fl_str_mv Román Cárdenas, Franklin
Ramón-Contento, Pablo
author Román Cárdenas, Franklin
author_facet Román Cárdenas, Franklin
Ramón-Contento, Pablo
author_role author
author2 Ramón-Contento, Pablo
author2_role author
dc.subject.none.fl_str_mv Brucella spp.
aborto
zoonosis
PCR
diagnóstico molecular
Brucella spp.
abortion
zoonosis
PCR
molecular diagnosis
topic Brucella spp.
aborto
zoonosis
PCR
diagnóstico molecular
Brucella spp.
abortion
zoonosis
PCR
molecular diagnosis
description Brucellosis is an infectious disease caused by microorganisms of the genus Brucella that affect different species of mammals.  The bacteria cause abortion, epididymitis, vesiculitis, birth of weak calves, the decrease in milk production, and infertility in cows and bulls. The disease is a zoonosis and is considered a public health problem. The objective of this work was to identify Brucella abortus by the Polymerase Chain Reaction. It was possible through the amplification of a fragment of 223 bp of the BCSP31 gene region, as DNA source, obtained from lymph nodes of the region cervical, pulmonary, bronchial, retropharyngeal, and mediastinal. One hundred and fifteen samples from animals slaughtered at Loja city abattoir in 2015 were analyzed. In 14.8% (17) of the slaughtered animals, the test was positive; although the presence of the bacteria was observed more frequently in the cervical, bronchial and retropharyngeal regions. The age of the positive animals ranged between 4 and 6 years. With the present study, it was confirmed the presence of the bacterium Brucella abortus through the molecular PCR test obtained from lymph nodes of slaughtered animals in the Loja canton.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242
10.29166/siembra.v8i1.2242
url https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242
identifier_str_mv 10.29166/siembra.v8i1.2242
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3591
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/4039
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3610
https://revistadigital.uce.edu.ec/index.php/SIEMBRA/article/view/2242/3611
dc.rights.none.fl_str_mv Derechos de autor 2021 Franklin Román Cárdenas, Pablo Ramón-Contento
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2021 Franklin Román Cárdenas, Pablo Ramón-Contento
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/zip
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dc.publisher.none.fl_str_mv Universidad Central del Ecuador
publisher.none.fl_str_mv Universidad Central del Ecuador
dc.source.none.fl_str_mv Siembra; Vol. 8 No. 1 (2021): SIEMBRA; e2242
Siembra; Vol. 8 Núm. 1 (2021): SIEMBRA; e2242
2477-8850
1390-8928
10.29166/siembra.v8i1
reponame:Revista Siembra
instname:Universidad Central del Ecuador
instacron:UCE
instname_str Universidad Central del Ecuador
instacron_str UCE
institution UCE
reponame_str Revista Siembra
collection Revista Siembra
repository.name.fl_str_mv Revista Siembra - Universidad Central del Ecuador
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