Application of Trichoderma harzianum for the control of bacterial cancer (Clavibacter michiganensis subsp. michiganensis) of tomato (Lycopersicum esculentum mill.) in a greenhouse
Five isolates of Trichoderma harzianum were evaluated under greenhouse conditions, these strains were collected from La Plata tomato fields (Buenos Aires, Argentina) and tested in vitro against of Clavibacter michiganensis subsp. Michiganensis, pathogen of the bacterial canker of tomato. The yt isola...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Ecuador |
| Institución: | Universidad Estatal Amazónica |
| Repositorio: | Revista Amazónica. Ciencia y Tecnología |
| Idioma: | español |
| OAI Identifier: | oai:ojs_revista.www.uea.edu.ec:article/87 |
| Acceso en línea: | https://revistas.uea.edu.ec/index.php/racyt/article/view/87 |
| Access Level: | acceso abierto |
| Palabra clave: | Biocontrol enfermedad tomate Trichoderma cáncer disease tomato Trichoderma koningiopsis canker |
| Sumario: | Five isolates of Trichoderma harzianum were evaluated under greenhouse conditions, these strains were collected from La Plata tomato fields (Buenos Aires, Argentina) and tested in vitro against of Clavibacter michiganensis subsp. Michiganensis, pathogen of the bacterial canker of tomato. The yt isolates were inoculated in 15 days seedlings using two methods: (1) soil inoculation during the transplant and (2) leaf sprayed three days before pathogen inoculation. Ten days after the transplant, the pathogen was inoculated by spraying a bacterial suspension with 1 x 107 CFU/ml, approximately. Five weeks after the transplant plant height, number of flowers and fruits were evaluated; results showed there were statistical differences between biocontrol treatments and the control. Tomato seedlings with leaves treated had bigger averages than those with soil inoculation. After eight weeks from transplant, disease symptomatology was evaluated. Approximately, 50% of pathogen control plants showed symptoms associated with the pathogen under glasshouse conditions, such us: marginal necrosis of leaflets and generalized slight wilting; the biocontrol strains had a positive effect over treated seedlings which were healthier than control ones, as well as they had better vegetative development and dry mass accumulation. |
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