Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens

Introduction. The Bacillus genus has been used as a biostimulant with positive effects on crop yield and quality. However, there are few studies on its application in microgreens. Objective. To evaluate the effect of B. subtilis (BsC4 and BsPC) and B. thuringiensis (Bt24) as biostimulants on the ger...

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Authors: Gutiérrez-Chávez, Aldo, Acebedo-Barrera, Angélica Anahí, Yañez-Muñoz, Rosa María, Hernandez Huerta, Jared
Format: article
Status:Published version
Publication Date:2025
Country:Costa Rica
Institution:Universidad de Costa Rica
Repository:Portal de Revistas UCR
Language:Spanish
OAI Identifier:oai:portal.ucr.ac.cr:article/61996
Online Access:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/61996
Access Level:Open access
Keyword:bioestimulantes
brásicas
microvegetales
rendimiento
biostimulants
brassicas
microgreens
yield
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oai_identifier_str oai:portal.ucr.ac.cr:article/61996
network_acronym_str CR
network_name_str Costa Rica
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dc.title.none.fl_str_mv Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
Efecto de Bacillus spp. como bioestimulante de microvegetales de Brassica oleracea var. capitata y Brassica oleracea var. sabellica
title Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
spellingShingle Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
Gutiérrez-Chávez, Aldo
bioestimulantes
brásicas
microvegetales
rendimiento
biostimulants
brassicas
microgreens
yield
title_short Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
title_full Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
title_fullStr Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
title_full_unstemmed Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
title_sort Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreens
dc.creator.none.fl_str_mv Gutiérrez-Chávez, Aldo
Acebedo-Barrera, Angélica Anahí
Yañez-Muñoz, Rosa María
Hernandez Huerta, Jared
author Gutiérrez-Chávez, Aldo
author_facet Gutiérrez-Chávez, Aldo
Acebedo-Barrera, Angélica Anahí
Yañez-Muñoz, Rosa María
Hernandez Huerta, Jared
author_role author
author2 Acebedo-Barrera, Angélica Anahí
Yañez-Muñoz, Rosa María
Hernandez Huerta, Jared
author2_role author
author
author
dc.subject.none.fl_str_mv bioestimulantes
brásicas
microvegetales
rendimiento
biostimulants
brassicas
microgreens
yield
topic bioestimulantes
brásicas
microvegetales
rendimiento
biostimulants
brassicas
microgreens
yield
description Introduction. The Bacillus genus has been used as a biostimulant with positive effects on crop yield and quality. However, there are few studies on its application in microgreens. Objective. To evaluate the effect of B. subtilis (BsC4 and BsPC) and B. thuringiensis (Bt24) as biostimulants on the germination and growth of red cabbage (Brassica oleracea var. capitata) and kale (B. oleracea var. sabellina) microgreens indoor. Materials and Methods. The study was conducted at the Autonomous University of Chihuahua, Mexico, in 2023. Four treatments were assessed: Bt24, BsC4, BsPC, and seedlings without microorganisms (control) on red cabbage and kale seeds, under a completely randomized design (n = 5). Germination was monitored in the first trial, and microgreens development was assessed in the secund. After 15 days, morphological variables, photosynthetic pigments, nitrate content, quality, and yield were measured. Data were analyzed using Analysis of Variance (ANOVA) and Tukey tests (p < 0.05). Results. For kale, Bt24 improved germination speed (31.17 %) and reduced germination time (4.61 %), with no significant effect on red cabbage. In kale, yield increased (62,60 %) with Bt24, particularly in fresh biomass (63.3 %) and cotyledon area (61.57 %). In cabbage, BsC4 increased yield (44.31 %) and improved fresh biomass (42.21 %) and cotyledon area (50.57 %). Nitrate content was highest in lake with BsPC (138.30 %) and in red cabbage with Bt24 (65.14 %), without exceeding safe limits. For visual quality, both crops achieved the “Good” and “Excellent” categories, with no differences between treatments. Conclusions. These results suggest that B. thuringiensis and B. subtilis could be used as growth biostimulants for cabbage and kale microgreens, offering a promising to optimize microgreen production under controlled conditions.
publishDate 2025
dc.date.none.fl_str_mv 2025-03-31
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
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artículo original
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info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/61996
10.15517/am.2025.61996
url https://revistas.ucr.ac.cr/index.php/agromeso/article/view/61996
identifier_str_mv 10.15517/am.2025.61996
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dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/61996/63674
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
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info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Agronomía Mesoamericana; Early view; 61996
Agronomía Mesoamericana; Visualización anticipada; 61996
Agronomía Mesoamericana; Visualización anticipada; 61996
2215-3608
1021-7444
reponame:Portal de Revistas UCR
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Effect of Bacillus spp. as a biostimulants for Brassica oleracea var. capitata and Brassica oleracea var. sabellica microgreensEfecto de Bacillus spp. como bioestimulante de microvegetales de Brassica oleracea var. capitata y Brassica oleracea var. sabellicaGutiérrez-Chávez, AldoAcebedo-Barrera, Angélica AnahíYañez-Muñoz, Rosa MaríaHernandez Huerta, JaredbioestimulantesbrásicasmicrovegetalesrendimientobiostimulantsbrassicasmicrogreensyieldIntroduction. The Bacillus genus has been used as a biostimulant with positive effects on crop yield and quality. However, there are few studies on its application in microgreens. Objective. To evaluate the effect of B. subtilis (BsC4 and BsPC) and B. thuringiensis (Bt24) as biostimulants on the germination and growth of red cabbage (Brassica oleracea var. capitata) and kale (B. oleracea var. sabellina) microgreens indoor. Materials and Methods. The study was conducted at the Autonomous University of Chihuahua, Mexico, in 2023. Four treatments were assessed: Bt24, BsC4, BsPC, and seedlings without microorganisms (control) on red cabbage and kale seeds, under a completely randomized design (n = 5). Germination was monitored in the first trial, and microgreens development was assessed in the secund. After 15 days, morphological variables, photosynthetic pigments, nitrate content, quality, and yield were measured. Data were analyzed using Analysis of Variance (ANOVA) and Tukey tests (p < 0.05). Results. For kale, Bt24 improved germination speed (31.17 %) and reduced germination time (4.61 %), with no significant effect on red cabbage. In kale, yield increased (62,60 %) with Bt24, particularly in fresh biomass (63.3 %) and cotyledon area (61.57 %). In cabbage, BsC4 increased yield (44.31 %) and improved fresh biomass (42.21 %) and cotyledon area (50.57 %). Nitrate content was highest in lake with BsPC (138.30 %) and in red cabbage with Bt24 (65.14 %), without exceeding safe limits. For visual quality, both crops achieved the “Good” and “Excellent” categories, with no differences between treatments. Conclusions. These results suggest that B. thuringiensis and B. subtilis could be used as growth biostimulants for cabbage and kale microgreens, offering a promising to optimize microgreen production under controlled conditions.Introducción. El género Bacillus se ha empleado como bioestimulante con efectos positivos sobre el rendimiento y la calidad de los cultivos. Sin embargo, existen pocos estudios sobre su aplicación en microvegetales. Objetivo. Evaluar el efecto de Bacillus subtilis (BsC4 y BsPC) y B. thuringiensis (Bt24) como bioestimulantes de la germinación y desarrollo de microvegetales de col morada (Brassica oleracea var. capitata) y kale (B. oleracea var. sabellica) en condiciones controladas. Materiales y métodos. El estudio se realizó en la Universidad Autónoma de Chihuahua, México, durante 2023. Se evaluaron cuatro tratamientos: Bt24, BsC4, BsPC y plántulas sin microorganismos (control) en semillas de col morada y kale, bajo un diseño completamente al azar (n = 5). En un primer ensayo se monitorizó la germinación y en un segundo el desarrollo de los microvegetales, después de 15 días se midieron variables morfológicas, pigmentos fotosintéticos, contenido de nitratos, calidad y rendimiento. Los datos se analizaron con Análisis de Varianzas (ANDEVA) y pruebas de Tukey (p < 0,05). Resultados. Para kale, Bt24 mejoró la velocidad de germinación (31,17 %) y redujo el tiempo de germinación (4,61 %), sin efecto significativo en col morada. En kale, el rendimiento aumentó (62,60 %) con Bt24 , destacando biomasa fresca (63,30 %) y área cotiledonar (61,57 %). En col morada, BsC4 incrementó el rendimiento (44,31 %), además de mejorar biomasa fresca (42,21 %) y área cotiledonar (50,57 % ). El contenido de nitratos fue más elevado en kale con BsPC (138,30 %) y en col morada con Bt24 (65,14 %), sin exceder límites seguros. En calidad visual, ambos cultivos alcanzaron categorías “Bueno” y “Excelente”, sin diferencias entre tratamientos. Conclusiones. Estos resultados sugieren que B. thuringiensis y B. subtillis podrían emplearse como bioestimulantes del crecimiento de microvegetales de col morada y kale como herramienta prometedora para optimizar su producción en condiciones controladas.Universidad de Costa Rica2025-03-31info:eu-repo/semantics/publishedVersionArticleTextTextoartículo originalhttp://purl.org/coar/resource_type/c_18cfhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/6199610.15517/am.2025.61996Agronomía Mesoamericana; Early view; 61996 Agronomía Mesoamericana; Visualización anticipada; 61996Agronomía Mesoamericana; Visualización anticipada; 619962215-36081021-7444reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspahttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/61996/63674Derechos de autor 2025 Aldo Gutiérrez-Chávez, Angélica Anahí Acebedo-Barrera, Rosa María Yañez-Muñoz, Jared Hernandez Huertahttps://creativecommons.org/licenses/by-nc-nd/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2025-02-03T13:25:46Zoai:portal.ucr.ac.cr:article/61996Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:30:17.101Portal de Revistas UCR - Universidad de Costa Ricafalse
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