Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile
Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to eva...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | Costa Rica |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/58892 |
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892 |
| Access Level: | acceso abierto |
| Palabra clave: | Stem cells; Dental stem cells; Dental pulp; Periodontal ligament; Adipogenesis. Células troncales; Células troncales dentales; Pulpa dental; Ligamento periodontal; Adipogénesis. |
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Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile Análisis comparativo de células troncales de origen dental de un donador: diferencias en la diferenciación adipogénica y su perfil protéico |
| title |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| spellingShingle |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile Marín-Uc, José A. Stem cells; Dental stem cells; Dental pulp; Periodontal ligament; Adipogenesis. Células troncales; Células troncales dentales; Pulpa dental; Ligamento periodontal; Adipogénesis. |
| title_short |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| title_full |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| title_fullStr |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| title_full_unstemmed |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| title_sort |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile |
| dc.creator.none.fl_str_mv |
Marín-Uc, José A. Aguilar-Hernández, Víctor Hernández-Sotomayor, Teresa Brito Argáez, Ligia Nic-Can, Geovanny I. Chuc-Gamboa, Martha Gabriela Aguilar-Ayala, Fernando Aguilar-Pérez, Fernando Rodas-Junco, Beatriz A. |
| author |
Marín-Uc, José A. |
| author_facet |
Marín-Uc, José A. Aguilar-Hernández, Víctor Hernández-Sotomayor, Teresa Brito Argáez, Ligia Nic-Can, Geovanny I. Chuc-Gamboa, Martha Gabriela Aguilar-Ayala, Fernando Aguilar-Pérez, Fernando Rodas-Junco, Beatriz A. |
| author_role |
author |
| author2 |
Aguilar-Hernández, Víctor Hernández-Sotomayor, Teresa Brito Argáez, Ligia Nic-Can, Geovanny I. Chuc-Gamboa, Martha Gabriela Aguilar-Ayala, Fernando Aguilar-Pérez, Fernando Rodas-Junco, Beatriz A. |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Stem cells; Dental stem cells; Dental pulp; Periodontal ligament; Adipogenesis. Células troncales; Células troncales dentales; Pulpa dental; Ligamento periodontal; Adipogénesis. |
| topic |
Stem cells; Dental stem cells; Dental pulp; Periodontal ligament; Adipogenesis. Células troncales; Células troncales dentales; Pulpa dental; Ligamento periodontal; Adipogénesis. |
| description |
Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to evaluate the differences in growth characteristics, expression of mesenchymal stem cells (MSCs)-specific markers, and the proteomic profile in response to adipogenic differentiation, of dental pulp and periodontal ligament cells obtained from a single donor. Dental cells were isolated from the third molar of a single donor using the outgrowth method. To obtain the proliferation curve of the cells was evaluated by trypan blue analysis. After the cells were cultured in adipogenic medium, morphological changes were monitored by oil red O staining, as well as adipogenic markers PPARγ and adiponectin by RT-qPCR. Finally, a two-dimensional electrophoresis of the proteins isolated from these cells was performed to analyze the proteomic profile. The two types of DSCs share similar cellular characteristics; however, their capacity for adipogenic differentiation is different. Based on the protein profiling results, we identified five differentially expressed proteins between both types of stem cells. Our findings showed that dental pulp and periodontal ligament stem cells from a single donor have similar cellular characteristics but a different response to adipogenesis, which would explain the differences in the expression of their proteins. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-08-30 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion Article artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892 10.15517/ijds.2024.58892 |
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https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892 |
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10.15517/ijds.2024.58892 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59159 https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59160 https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59161 |
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https://creativecommons.org/licenses/by-nc-sa/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf text/html application/epub+zip |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Odovtos - International Journal of Dental Sciences; Vol. 26 No. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 123-139 Odovtos International Journal of Dental Sciences; Vol. 26 Núm. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 123-139 2215-3411 1659-1046 10.15517/ijds.2024 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
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Universidad de Costa Rica |
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UCR |
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UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR - Universidad de Costa Rica |
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jorge.polanco@ucr.ac.cr |
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1849325446264520704 |
| spelling |
Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein ProfileAnálisis comparativo de células troncales de origen dental de un donador: diferencias en la diferenciación adipogénica y su perfil protéicoMarín-Uc, José A.Aguilar-Hernández, VíctorHernández-Sotomayor, TeresaBrito Argáez, LigiaNic-Can, Geovanny I. Chuc-Gamboa, Martha GabrielaAguilar-Ayala, FernandoAguilar-Pérez, FernandoRodas-Junco, Beatriz A.Stem cells; Dental stem cells; Dental pulp; Periodontal ligament; Adipogenesis.Células troncales; Células troncales dentales; Pulpa dental; Ligamento periodontal; Adipogénesis.Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to evaluate the differences in growth characteristics, expression of mesenchymal stem cells (MSCs)-specific markers, and the proteomic profile in response to adipogenic differentiation, of dental pulp and periodontal ligament cells obtained from a single donor. Dental cells were isolated from the third molar of a single donor using the outgrowth method. To obtain the proliferation curve of the cells was evaluated by trypan blue analysis. After the cells were cultured in adipogenic medium, morphological changes were monitored by oil red O staining, as well as adipogenic markers PPARγ and adiponectin by RT-qPCR. Finally, a two-dimensional electrophoresis of the proteins isolated from these cells was performed to analyze the proteomic profile. The two types of DSCs share similar cellular characteristics; however, their capacity for adipogenic differentiation is different. Based on the protein profiling results, we identified five differentially expressed proteins between both types of stem cells. Our findings showed that dental pulp and periodontal ligament stem cells from a single donor have similar cellular characteristics but a different response to adipogenesis, which would explain the differences in the expression of their proteins.Las células troncales dentales (CTDs) son células multipotentes con gran capacidad de proliferación y diferenciación multilinaje. Pocos estudios han comparado las características celulares y el potencial de diferenciación adipogénica de las CTDs derivadas de tejidos de un mismo individuo. El objetivo de este trabajo fue evaluar las diferencias en las características de crecimiento, la expresión de marcadores específicos de celulas troncales mesenquimales (CTMs) y el perfil de proteínas en respuesta a la diferenciación adipogénica, de células de pulpa dental y ligamento periodontal obtenidas de un mismo donante. Las células dentales se aislaron a partir del tercer molar de un único donante mediante el método de explante. Para obtener la curva de proliferación de las células se evaluó mediante análisis con azul tripano. Tras cultivar las células en medio adipogénico, se controlaron los cambios morfológicos mediante tinción con rojo O oleoso, así como los marcadores adipogénicos PPARγ y adiponectina mediante RT-qPCR. Por último, se realizó una electroforesis bidimensional de las proteínas aisladas de estas células para analizar el perfil proteómico. Los dos tipos de CTDs comparten características celulares similares; sin embargo, su capacidad de diferenciación adipogénica es diferente. Basándonos en los resultados del perfil proteico, identificamos cinco proteínas expresadas diferencialmente entre ambos tipos de células troncales. Los resultados mostraron que las células troncales de la pulpa dental y del ligamento periodontal de un mismo donante tienen características celulares similares pero una respuesta diferente a la adipogénesis, lo que explicaría las diferencias en la expresión de sus proteinas.Universidad de Costa Rica2024-08-30info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/epub+ziphttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/5889210.15517/ijds.2024.58892Odovtos - International Journal of Dental Sciences; Vol. 26 No. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 123-139Odovtos International Journal of Dental Sciences; Vol. 26 Núm. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 123-1392215-34111659-104610.15517/ijds.2024reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59159https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59160https://revistas.ucr.ac.cr/index.php/Odontos/article/view/58892/59161Derechos de autor 2024 José A. Marín-Uc, Víctor Aguilar-Hernández, Teresa Hernández-Sotomayor, Ligia Brito Argáez, Geovanny I. Nic-Can, Martha Gabriela Chuc-Gamboa, Fernando Aguilar-Ayala, Fernando Aguilar-Pérez, Beatriz A. Rodas-Juncohttps://creativecommons.org/licenses/by-nc-sa/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2025-01-17T19:11:10Zoai:portal.ucr.ac.cr:article/58892Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:44:39.694Portal de Revistas UCR - Universidad de Costa Ricafalse |
| score |
15,812429 |