Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).

The Guarianthe skinneri orchid is included in NOM-059-ECOL-2010, Mexico standard as an endangered species. In order to study PGPR (promoting growth plant rhizobacteria) from this orchid, 10 roots were collected from different plants to isolate bacteria associated with the roots, which were analyzed...

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Detalhes bibliográficos
Autores: Aguilar Diaz, Trinidad, Bertolini, Vincenzo, Carrillo Castañeda, Guillermo, Guillén Navarro, Griselda Karina, García Fajardo, Luz Verónica, Castro Chan, Ricardo Alberto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Costa Rica
Recursos:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:español
OAI Identifier:oai:portal.ucr.ac.cr:article/30638
Acesso em linha:https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638
Access Level:acceso abierto
Palavra-chave:Bacillus sp.
antagonism
indoleacetic acid
phosphate solubilization
nitrogen fixation.
Bacillus sp
antagonismo
ácido indolacético
solubilización de fosfato
fijación de nitrógeno
id CR_dcc17f30d43d286fffa0eff3c1a8c3ec
oai_identifier_str oai:portal.ucr.ac.cr:article/30638
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.none.fl_str_mv Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
Rizobacterias promotoras de crecimiento en Guarianthe skinneri (Orchidaceae)
title Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
spellingShingle Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
Aguilar Diaz, Trinidad
Bacillus sp.
antagonism
indoleacetic acid
phosphate solubilization
nitrogen fixation.
Bacillus sp
antagonismo
ácido indolacético
solubilización de fosfato
fijación de nitrógeno
title_short Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
title_full Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
title_fullStr Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
title_full_unstemmed Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
title_sort Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).
dc.creator.none.fl_str_mv Aguilar Diaz, Trinidad
Bertolini, Vincenzo
Carrillo Castañeda, Guillermo
Guillén Navarro, Griselda Karina
García Fajardo, Luz Verónica
Castro Chan, Ricardo Alberto
author Aguilar Diaz, Trinidad
author_facet Aguilar Diaz, Trinidad
Bertolini, Vincenzo
Carrillo Castañeda, Guillermo
Guillén Navarro, Griselda Karina
García Fajardo, Luz Verónica
Castro Chan, Ricardo Alberto
author_role author
author2 Bertolini, Vincenzo
Carrillo Castañeda, Guillermo
Guillén Navarro, Griselda Karina
García Fajardo, Luz Verónica
Castro Chan, Ricardo Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus sp.
antagonism
indoleacetic acid
phosphate solubilization
nitrogen fixation.
Bacillus sp
antagonismo
ácido indolacético
solubilización de fosfato
fijación de nitrógeno
topic Bacillus sp.
antagonism
indoleacetic acid
phosphate solubilization
nitrogen fixation.
Bacillus sp
antagonismo
ácido indolacético
solubilización de fosfato
fijación de nitrógeno
description The Guarianthe skinneri orchid is included in NOM-059-ECOL-2010, Mexico standard as an endangered species. In order to study PGPR (promoting growth plant rhizobacteria) from this orchid, 10 roots were collected from different plants to isolate bacteria associated with the roots, which were analyzed by in vitro tests such as: production of AIA, nitrogen fixation, interaction with the mycorrhizal fungus Thanatephorus sp. strain RG26 and phosphate solubilization. We obtain 71 bacterial isolates, 10 strains of them were characterized by sequencing with the 16d rDNA marker identifying six bacteria: Sphingomonas sp. Sinorhizobium sp. Bacillus sp. Nocardia cerradoensis, Bacillus megaterium and Burkholderia phytofirmans. We observed that the bacterium Sinorhizobium sp. produced a greater amount of AIA (69.189 μg/ml) and Bacillus sp. performed greater acetylene reduction (10.251 nmol cultivo/96h). In the interactions of the bacteria and the fungus RG26, four categories were presented (extremely positive, positive, antagonism 50-50 and inhibition). In relation to the solubilization of phosphate, Burkholderia phytofirmans presented higher IS after 48 and 96 hr with an IS of 3.11 and 3.48, respectively. The results indicate that Bacillus sp. it could have the best characteristics to promote the development of the G. skinneri orchid by inoculating seeds and seedlings.  
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638
10.15517/rbt.v66i3.30638
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638
identifier_str_mv 10.15517/rbt.v66i3.30638
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/33809
https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/33810
https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/35531
dc.rights.none.fl_str_mv Derechos de autor 2018 Revista de Biología Tropical
http://creativecommons.org/licenses/by/4.0
acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2018 Revista de Biología Tropical
http://creativecommons.org/licenses/by/4.0
acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Revista de Biología Tropical; Vol. 66 No. 3 (2018): Volume 66 – Regular number 3 – September 2018; 953–968
Revista de Biología Tropical; Vol. 66 Núm. 3 (2018): Volumen 66 – Número regular 3 – Setiembre 2018; 953–968
Revista Biología Tropical; Vol. 66 N.º 3 (2018): Volumen 66 – Número regular 3 – Setiembre 2018; 953–968
2215-2075
0034-7744
10.15517/rbt.v66i3
reponame:Portal de Revistas UCR
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae).Rizobacterias promotoras de crecimiento en Guarianthe skinneri (Orchidaceae)Aguilar Diaz, TrinidadBertolini, VincenzoCarrillo Castañeda, GuillermoGuillén Navarro, Griselda KarinaGarcía Fajardo, Luz VerónicaCastro Chan, Ricardo AlbertoBacillus sp.antagonismindoleacetic acidphosphate solubilizationnitrogen fixation.Bacillus spantagonismoácido indolacéticosolubilización de fosfatofijación de nitrógenoThe Guarianthe skinneri orchid is included in NOM-059-ECOL-2010, Mexico standard as an endangered species. In order to study PGPR (promoting growth plant rhizobacteria) from this orchid, 10 roots were collected from different plants to isolate bacteria associated with the roots, which were analyzed by in vitro tests such as: production of AIA, nitrogen fixation, interaction with the mycorrhizal fungus Thanatephorus sp. strain RG26 and phosphate solubilization. We obtain 71 bacterial isolates, 10 strains of them were characterized by sequencing with the 16d rDNA marker identifying six bacteria: Sphingomonas sp. Sinorhizobium sp. Bacillus sp. Nocardia cerradoensis, Bacillus megaterium and Burkholderia phytofirmans. We observed that the bacterium Sinorhizobium sp. produced a greater amount of AIA (69.189 μg/ml) and Bacillus sp. performed greater acetylene reduction (10.251 nmol cultivo/96h). In the interactions of the bacteria and the fungus RG26, four categories were presented (extremely positive, positive, antagonism 50-50 and inhibition). In relation to the solubilization of phosphate, Burkholderia phytofirmans presented higher IS after 48 and 96 hr with an IS of 3.11 and 3.48, respectively. The results indicate that Bacillus sp. it could have the best characteristics to promote the development of the G. skinneri orchid by inoculating seeds and seedlings.  La orquídea Guarianthe skinneri está incluida en la norma NOM-059-ECOL-2010 de México como una especie amenazada. Con el fin de estudiar las BPCV (bacterias promotoras del crecimiento vegetal) en esta orquídea, se recolectaron 10 raíces de diferentes plantas para aislar bacterias asociadas a las raíces, que se analizaron mediante pruebas in vitro como: producción de AIA, fijación de nitrógeno, interacción con el hongo micorrízico Thanatephorus sp. cepa RG26 y solubilización de fosfato. De los 71 aislados bacterianos se caracterizaron 10 cepas mediante secuenciación con el marcador 16s rADN y se identificaron seis cepas: Sphingomonas sp., Sinorhizobium sp., Bacillus sp., Nocardia cerradoensis, Bacillus megaterium y Burkholderia phytofirmans. Se observó que la bacteria Sinorhizobium sp. produjo mayor cantidad de AIA (69.189 µg/ml) y Bacillus sp. presentó mayor reducción de acetileno (10.251 nmol cultivo/96 h). En las interacciones de las bacterias y el hongo RG26 se presentaron cuatro categorías (sumamente positivo, positivo, antagonismo 50-50 e inhibición). En relación a la solubilización de fosfato, la bacteria Burkholderia phytofirmans presentó mayor IS a las 48 y 96 hr con IS de 3.11 y 3.48, respectivamente. Los resultados indican que Bacillus sp. pudiera tener las mejores características para promover el desarrollo de la orquídea G. skinneri mediante la inoculación de semillas y plántulas.Universidad de Costa Rica2018-09-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/pdfhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/3063810.15517/rbt.v66i3.30638Revista de Biología Tropical; Vol. 66 No. 3 (2018): Volume 66 – Regular number 3 – September 2018; 953–968Revista de Biología Tropical; Vol. 66 Núm. 3 (2018): Volumen 66 – Número regular 3 – Setiembre 2018; 953–968Revista Biología Tropical; Vol. 66 N.º 3 (2018): Volumen 66 – Número regular 3 – Setiembre 2018; 953–9682215-20750034-774410.15517/rbt.v66i3reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspahttps://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/33809https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/33810https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638/35531Derechos de autor 2018 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-09T18:15:42Zoai:portal.ucr.ac.cr:article/30638Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:23:53.544Portal de Revistas UCR - Universidad de Costa Ricafalse
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