Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves
Toxoplasma gondii is an obligatory intracellular parasite that infects a wide range of warm-blooded animals and humans. Considering the severity of toxoplasmosis, side effects of current treatments, and the contribution of the ethnopharmacological knowledge for the treatment of parasitic infections,...
| Autores: | , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2015 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositório: | Portal de Revistas UCR |
| Idioma: | inglês |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/14545 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545 |
| Access Level: | Acceso aberto |
| Palavra-chave: | anti-Toxoplasma Sambucus nigra Toxoplasma gondii methanolic extract in vitro extracto metanólico |
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Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves Actividad anti-Toxoplasma de extractos metanólicos de frutos y hojas de Sambucus nigra (Caprifoliaceae) |
| title |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| spellingShingle |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves Daryani, Ahmad anti-Toxoplasma Sambucus nigra Toxoplasma gondii methanolic extract in vitro anti-Toxoplasma Sambucus nigra Toxoplasma gondii extracto metanólico in vitro |
| title_short |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| title_full |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| title_fullStr |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| title_full_unstemmed |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| title_sort |
Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leaves |
| dc.creator.none.fl_str_mv |
Daryani, Ahmad Ebrahimzadeh, Mohammad Ali Sharif, Mehdi Ahmadpour, Ehsan Edalatian, Sara Rahimi Esboei, Bahman Sarvi, Shahabeddin |
| author |
Daryani, Ahmad |
| author_facet |
Daryani, Ahmad Ebrahimzadeh, Mohammad Ali Sharif, Mehdi Ahmadpour, Ehsan Edalatian, Sara Rahimi Esboei, Bahman Sarvi, Shahabeddin |
| author_role |
author |
| author2 |
Ebrahimzadeh, Mohammad Ali Sharif, Mehdi Ahmadpour, Ehsan Edalatian, Sara Rahimi Esboei, Bahman Sarvi, Shahabeddin |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
anti-Toxoplasma Sambucus nigra Toxoplasma gondii methanolic extract in vitro anti-Toxoplasma Sambucus nigra Toxoplasma gondii extracto metanólico in vitro |
| topic |
anti-Toxoplasma Sambucus nigra Toxoplasma gondii methanolic extract in vitro anti-Toxoplasma Sambucus nigra Toxoplasma gondii extracto metanólico in vitro |
| description |
Toxoplasma gondii is an obligatory intracellular parasite that infects a wide range of warm-blooded animals and humans. Considering the severity of toxoplasmosis, side effects of current treatments, and the contribution of the ethnopharmacological knowledge for the treatment of parasitic infections, the aim of the present study was to investigate the efficacy of methanolic extracts from the fruits and leaves of Sambucus nigra against tachyzoite of T. gondii. For this, fruits and leaves of S. nigra were collected from Mazandaran province, Iran, were dried under the shade, and powdered using a commercial electrical blender. For extractions, methanol was used as solvent. Virulent RH strain of T. gondii was maintained in mice and macrophages containing tachyzoites were aspirated from the peritoneal cavity. Four concentrations (5, 10, 25 and 50mg/mL) of S. nigra extract were incubated with infected macrophages for 30, 60, 120 and 180 minutes and the viability of the tachyzoites were evaluated by trypan blue staining. Results showed that S. nigra fruit extracts at the concentrations of 5 and 10mg/mL killed 100% of T. gondii tachyzoites after 60 and 120 minutes, respectively; and concentrations of 25 and 50mg/mL killed 100% of the tachyzoites after 30 minutes. Additionally, extract of S. nigra leaves, at the concentrations of 5, 10 and 25mg/mLafter 180 minutes, and concentration of 50mg/mLafter 60 minutes, resulted with the highest efficacy. Our results showed that S. nigra has acceptable efficacy in vitro and the parasiticidal effect of fruit extract was significantly better than leaf extract. However, in vivo efficacy of this extract needs further investigation. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-03-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545 10.15517/rbt.v63i1.14545 |
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https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545 |
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10.15517/rbt.v63i1.14545 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545/16843 https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545/16844 |
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Derechos de autor 2015 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
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Derechos de autor 2015 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
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Universidad de Costa Rica |
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Revista de Biología Tropical; Vol. 63 No. 1 (2015): Volume 63 – Regular number 1 – March 2015; 7–12 Revista de Biología Tropical; Vol. 63 Núm. 1 (2015): Volumen 63 – Número regular 1 – Marzo 2015; 7–12 Revista Biología Tropical; Vol. 63 N.º 1 (2015): Volumen 63 – Número regular 1 – Marzo 2015; 7–12 2215-2075 0034-7744 10.15517/rbt.v63i1 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
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Universidad de Costa Rica |
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UCR |
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UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR - Universidad de Costa Rica |
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jorge.polanco@ucr.ac.cr |
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1849325359706669056 |
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Anti-Toxoplasma activities of methanolic extract of Sambucus nigra (Caprifoliaceae) fruits and leavesActividad anti-Toxoplasma de extractos metanólicos de frutos y hojas de Sambucus nigra (Caprifoliaceae)Daryani, AhmadEbrahimzadeh, Mohammad AliSharif, MehdiAhmadpour, EhsanEdalatian, SaraRahimi Esboei, BahmanSarvi, Shahabeddinanti-ToxoplasmaSambucus nigraToxoplasma gondiimethanolic extractin vitroanti-ToxoplasmaSambucus nigraToxoplasma gondiiextracto metanólicoin vitroToxoplasma gondii is an obligatory intracellular parasite that infects a wide range of warm-blooded animals and humans. Considering the severity of toxoplasmosis, side effects of current treatments, and the contribution of the ethnopharmacological knowledge for the treatment of parasitic infections, the aim of the present study was to investigate the efficacy of methanolic extracts from the fruits and leaves of Sambucus nigra against tachyzoite of T. gondii. For this, fruits and leaves of S. nigra were collected from Mazandaran province, Iran, were dried under the shade, and powdered using a commercial electrical blender. For extractions, methanol was used as solvent. Virulent RH strain of T. gondii was maintained in mice and macrophages containing tachyzoites were aspirated from the peritoneal cavity. Four concentrations (5, 10, 25 and 50mg/mL) of S. nigra extract were incubated with infected macrophages for 30, 60, 120 and 180 minutes and the viability of the tachyzoites were evaluated by trypan blue staining. Results showed that S. nigra fruit extracts at the concentrations of 5 and 10mg/mL killed 100% of T. gondii tachyzoites after 60 and 120 minutes, respectively; and concentrations of 25 and 50mg/mL killed 100% of the tachyzoites after 30 minutes. Additionally, extract of S. nigra leaves, at the concentrations of 5, 10 and 25mg/mLafter 180 minutes, and concentration of 50mg/mLafter 60 minutes, resulted with the highest efficacy. Our results showed that S. nigra has acceptable efficacy in vitro and the parasiticidal effect of fruit extract was significantly better than leaf extract. However, in vivo efficacy of this extract needs further investigation.Toxoplasma gondii es un parásito intracelular obligatorio que infecta a una gran variedad de animales y seres humanos. Teniendo en cuenta la gravedad de la toxoplasmosis, los efectos secundarios de los tratamientos actuales, y la contribución de los conocimientos etnofarmacológicos para el tratamiento de infecciones parasitarias, el objetivo del presente estudio fue investigar la eficacia de los extractos metanólicos de los frutos y hojas de Sambucus nigra contra el taquizoito de T. gondii. Para esto, se recogieron frutos y hojas de S. nigra en la provincia de Mazandaran, Irán, se secaron a la sombra, y se pulverizaron con una batidora eléctrica comercial. Para las extracciones, se empleó metanol como disolvente. La cepa virulenta RH de T. gondii se mantuvo en ratones y los macrófagos con taquizoitos se aspiraron de la cavidad peritoneal. Cuatro concentraciones (5, 10, 25 y 50mg/ mL) de extracto de S. nigra se encubaron con los macrófagos infectados durante 30, 60, 120 y 180 minutos y la viabilidad de los taquizoitos se evaluó mediante tinción con azul de tripano. Los resultados mostraron que los extractos de frutos de S. nigra en las concentraciones de 5 y 10mg/mL mataron al 100% de los taquizoitos de T. gondii después de 60 y 120 minutos, respectivamente; y las concentraciones de 25 y 50mg/mL mataron al 100% de los taquizoitos después de 30 minutos. Además, el extracto de hojas de S. nigra, en concentraciones de 5, 10 y 25mg/mL después de 180 minutos, y una concentración de 50mg/mL después de 60 minutos, resultaron más eficientes. Nuestros resultados mostraron que S. nigra tiene una eficacia aceptable in vitro y el efecto parasiticida del extracto de frutos fue significativamente mejor que el del extracto de hoja. Sin embargo, la eficacia in vivo de este extracto necesita más investigación.Universidad de Costa Rica2015-03-01info:eu-repo/semantics/publishedVersionartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/1454510.15517/rbt.v63i1.14545Revista de Biología Tropical; Vol. 63 No. 1 (2015): Volume 63 – Regular number 1 – March 2015; 7–12Revista de Biología Tropical; Vol. 63 Núm. 1 (2015): Volumen 63 – Número regular 1 – Marzo 2015; 7–12Revista Biología Tropical; Vol. 63 N.º 1 (2015): Volumen 63 – Número regular 1 – Marzo 2015; 7–122215-20750034-774410.15517/rbt.v63i1reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/rbt/article/view/14545/16843https://revistas.ucr.ac.cr/index.php/rbt/article/view/14545/16844Derechos de autor 2015 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-09T17:42:18Zoai:portal.ucr.ac.cr:article/14545Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:16:20.996Portal de Revistas UCR - Universidad de Costa Ricafalse |
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15,811543 |