Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate

We report on a new amperometric assay for Al(III) ions based on the inhibition of α-chymotrypsin enzyme. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles and polyvinyl alcohol. N-acetyl-tyrosine ethyl ester, used as enzyme substrate,...

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Authors: Barquero Quirós, Miriam, Domínguez Renedo, Olga, Alonso Lomillo, María Asunción, Arcos Martínez, María Julia
Format: article
Publication Date:2015
Country:Costa Rica
Institution:Universidad de Costa Rica
Repository:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/80664
Online Access:https://www.semanticscholar.org/paper/Biosensor-for-Aluminum(III)-Based-on-α-Chymotrypsin-Barquero-Quirós-Domínguez-Renedo/b433b1bd9f3b31e5767f8f381345194572fd67c9
https://astonjournals.com/csjvols.html
https://hdl.handle.net/10669/80664
Access Level:Open access
Keyword:α-chymotrypsin
Acetyl-L-tyrosine ethyl ester
Biosensor
Aluminum
Screen-printed electrodes
Gold nanoparticles
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network_name_str Costa Rica
repository_id_str
dc.title.es_ES.fl_str_mv Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
title Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
spellingShingle Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
Barquero Quirós, Miriam
α-chymotrypsin
Acetyl-L-tyrosine ethyl ester
Biosensor
Aluminum
Screen-printed electrodes
Gold nanoparticles
title_short Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
title_full Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
title_fullStr Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
title_full_unstemmed Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
title_sort Biosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate
dc.creator.none.fl_str_mv Barquero Quirós, Miriam
Domínguez Renedo, Olga
Alonso Lomillo, María Asunción
Arcos Martínez, María Julia
author Barquero Quirós, Miriam
author_facet Barquero Quirós, Miriam
Domínguez Renedo, Olga
Alonso Lomillo, María Asunción
Arcos Martínez, María Julia
author_role author
author2 Domínguez Renedo, Olga
Alonso Lomillo, María Asunción
Arcos Martínez, María Julia
author2_role author
author
author
dc.subject.es_ES.fl_str_mv α-chymotrypsin
Acetyl-L-tyrosine ethyl ester
Biosensor
Aluminum
Screen-printed electrodes
Gold nanoparticles
topic α-chymotrypsin
Acetyl-L-tyrosine ethyl ester
Biosensor
Aluminum
Screen-printed electrodes
Gold nanoparticles
description We report on a new amperometric assay for Al(III) ions based on the inhibition of α-chymotrypsin enzyme. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles and polyvinyl alcohol. N-acetyl-tyrosine ethyl ester, used as enzyme substrate, produces an oxidation amperometric signal, which at increasing aluminum concentration is diminished. The developed system has a detection limit of 1.5 ± 0.1 μM (n=7) for Al(III). The reproducibility of the method is 6.6% (n=5) and its repeatability is 8.2% (n=3). Main interferences at low concentration, include As(V), Cd(II), and Mo(VI) ions. The developed method was successfully applied to the determination of Al(III) in spiked white wine sample. Results agree with the certified value giving a value of percent recovery of 103 ± 4% (n=4).
publishDate 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2020-02-28T20:09:03Z
dc.date.available.none.fl_str_mv 2020-02-28T20:09:03Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv https://www.semanticscholar.org/paper/Biosensor-for-Aluminum(III)-Based-on-α-Chymotrypsin-Barquero-Quirós-Domínguez-Renedo/b433b1bd9f3b31e5767f8f381345194572fd67c9
https://astonjournals.com/csjvols.html
dc.identifier.issn.none.fl_str_mv 2150-3494
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/80664
dc.identifier.doi.none.fl_str_mv 10.4172/2150-3494.100089
dc.identifier.codproyecto.none.fl_str_mv 804-B2-299
url https://www.semanticscholar.org/paper/Biosensor-for-Aluminum(III)-Based-on-α-Chymotrypsin-Barquero-Quirós-Domínguez-Renedo/b433b1bd9f3b31e5767f8f381345194572fd67c9
https://astonjournals.com/csjvols.html
https://hdl.handle.net/10669/80664
identifier_str_mv 2150-3494
10.4172/2150-3494.100089
804-B2-299
dc.language.iso.es_ES.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.source.es_ES.fl_str_mv Chemical Sciences Journal, vol.6(2), pp.1-6
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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spelling Barquero Quirós, Miriambc9ffe6e-e07e-4d84-ade9-e0d7b36fac00600Domínguez Renedo, Olga2685239a-f61b-4bd5-bfeb-f915186cafabAlonso Lomillo, María Asunción9a8de412-5c21-4f70-8c11-2f650c0402e1Arcos Martínez, María Julia68af356a-ad9c-48db-b510-f9b80b9062246002020-02-28T20:09:03Z2020-02-28T20:09:03Z2015https://www.semanticscholar.org/paper/Biosensor-for-Aluminum(III)-Based-on-α-Chymotrypsin-Barquero-Quirós-Domínguez-Renedo/b433b1bd9f3b31e5767f8f381345194572fd67c9https://astonjournals.com/csjvols.html2150-3494https://hdl.handle.net/10669/8066410.4172/2150-3494.100089804-B2-299We report on a new amperometric assay for Al(III) ions based on the inhibition of α-chymotrypsin enzyme. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles and polyvinyl alcohol. N-acetyl-tyrosine ethyl ester, used as enzyme substrate, produces an oxidation amperometric signal, which at increasing aluminum concentration is diminished. The developed system has a detection limit of 1.5 ± 0.1 μM (n=7) for Al(III). The reproducibility of the method is 6.6% (n=5) and its repeatability is 8.2% (n=3). Main interferences at low concentration, include As(V), Cd(II), and Mo(VI) ions. The developed method was successfully applied to the determination of Al(III) in spiked white wine sample. Results agree with the certified value giving a value of percent recovery of 103 ± 4% (n=4).Universidad de Costa Rica/[804-B2-299]/UCR/Costa RicaJunta de Castilla y León/[BU212A12-2]//EspañaMinisterio de Ciencia e Innovación/[]/MICINN/EspañaFondo Europeo de Desarrollo Regional/[INNPACTO SERIBIO 2011-2014]/FEDER/EspañaFondo Europeo de Desarrollo Regional/[TEC-2009/12029]/FEDER/EspañaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)en_USacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessChemical Sciences Journal, vol.6(2), pp.1-6reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRα-chymotrypsinAcetyl-L-tyrosine ethyl esterBiosensorAluminumScreen-printed electrodesGold nanoparticlesBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrateartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleLICENSElicense.txtlicense.txttext/plain; charset=utf-82901https://www.kerwa.ucr.ac.cr/bitstreams/8b9b425b-49ac-4945-9d0b-8644ec4c647f/downloadbc4aed01f809e5c64e810e000d6a48b8MD52ORIGINALBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdfBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdfapplication/pdf1010010https://www.kerwa.ucr.ac.cr/bitstreams/1550dcf1-74ed-4293-bcdc-f9c340d5f27e/download4ac18b2e9fc85656a2fc27901b023dedMD51TEXTBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdf.txtBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdf.txtExtracted texttext/plain35591https://www.kerwa.ucr.ac.cr/bitstreams/b64185a6-a0c4-4a53-9005-4e149ba451ab/download42565792bf825f02777f4c79aa741a68MD55THUMBNAILBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdf.jpgBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substrate.pdf.jpgIM Thumbnailimage/jpeg10863https://www.kerwa.ucr.ac.cr/bitstreams/ac533942-5eb4-4c93-bd21-b7d1780ebe3a/downloadfd5f9299ba9a973f67a50cb479c98f85MD5610669/806642024-06-21 10:13:14.659open.accessoai:kerwa.ucr.ac.cr:10669/80664https://www.kerwa.ucr.ac.crInstitucionalhttp://www.kerwa.ucr.ac.crUniversidadhttp://www.ucr.ac.crhttps://kerwa.ucr.ac.cr/oai/requestmeilyn.garro@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:18712025-03-29T18:10:26.889Kérwá - 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