Bothrops asper metalloproteinase BaP1 is inhibited by alpha(2)-macroglobulin and mouse serum and does not induce systemic hemorrhage or coagulopathy
The ability of the P-I metalloproteinase BaP1, isolated from the venom of the snake Bothrops asper, to induce systemic bleeding, thrombocytopenia and defibrinogenation was assessed in an experimental mouse model. Intravenous administration of BaP1 caused neither systemic bleeding nor any evidence of...
| Authors: | , , , , |
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| Format: | article |
| Publication Date: | 2004 |
| Country: | Costa Rica |
| Institution: | Universidad de Costa Rica |
| Repository: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/29511 |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0041010103003404 https://hdl.handle.net/10669/29511 |
| Access Level: | Embargoed access |
| Keyword: | Metalloproteinase Systemic Hemorrhage Coagulopathy Bothrops Asper Venom Snake venom |
| Summary: | The ability of the P-I metalloproteinase BaP1, isolated from the venom of the snake Bothrops asper, to induce systemic bleeding, thrombocytopenia and defibrinogenation was assessed in an experimental mouse model. Intravenous administration of BaP1 caused neither systemic bleeding nor any evidence of pathology in lungs, kidneys, liver, heart and brain. Moreover, there were no alterations in the whole blood clotting time or in platelet numbers. In addition, BaP1 did not inhibit collagen-induced platelet aggregation in vitro. Proteolytic and hemorrhagic activities of BaP1 were readily inhibited by the plasma proteinase inhibitor, α2-macroglobulin, and normal mouse serum also inhibited hemorrhage. Such inhibition may explain why BaP1 induces multiple local tissue-damaging effects, but is largely devoid of systemic toxicity. |
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