The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms

Bothrops asper is responsible for approximately half of the snakebite envenomations in Central America. Despite its medical relevance, only the venom of Costa Rican populations of this species has been studied to some detail, and there is very little information on intraspecies variability in venom...

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Detalles Bibliográficos
Autores: Saravia Otten, Patricia, Rojas Umaña, Ermila, Escalante Muñoz, Teresa, Arce, Viviana, Chaves Olarte, Esteban, Velásquez, Rubén, Lomonte, Bruno, Rojas Céspedes, Gustavo, Gutiérrez, José María
Tipo de recurso: artículo
Fecha de publicación:2001
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/29467
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S0041010100001227
https://hdl.handle.net/10669/29467
Access Level:acceso embargado
Palabra clave:Bothrops asper
Antivenom
Neutralization
Guatemala
Snake venom
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dc.title.es_ES.fl_str_mv The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
title The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
spellingShingle The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
Saravia Otten, Patricia
Bothrops asper
Antivenom
Neutralization
Guatemala
Snake venom
title_short The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
title_full The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
title_fullStr The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
title_full_unstemmed The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
title_sort The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms
dc.creator.none.fl_str_mv Saravia Otten, Patricia
Rojas Umaña, Ermila
Escalante Muñoz, Teresa
Arce, Viviana
Chaves Olarte, Esteban
Velásquez, Rubén
Lomonte, Bruno
Rojas Céspedes, Gustavo
Gutiérrez, José María
author Saravia Otten, Patricia
author_facet Saravia Otten, Patricia
Rojas Umaña, Ermila
Escalante Muñoz, Teresa
Arce, Viviana
Chaves Olarte, Esteban
Velásquez, Rubén
Lomonte, Bruno
Rojas Céspedes, Gustavo
Gutiérrez, José María
author_role author
author2 Rojas Umaña, Ermila
Escalante Muñoz, Teresa
Arce, Viviana
Chaves Olarte, Esteban
Velásquez, Rubén
Lomonte, Bruno
Rojas Céspedes, Gustavo
Gutiérrez, José María
author2_role author
author
author
author
author
author
author
author
dc.subject.es_ES.fl_str_mv Bothrops asper
Antivenom
Neutralization
Guatemala
Snake venom
topic Bothrops asper
Antivenom
Neutralization
Guatemala
Snake venom
description Bothrops asper is responsible for approximately half of the snakebite envenomations in Central America. Despite its medical relevance, only the venom of Costa Rican populations of this species has been studied to some detail, and there is very little information on intraspecies variability in venom composition and toxicity. Venom of B. asper from Guatemala was analyzed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis, and its basic pharmacological activities were investigated with standard laboratory assays. Venom has lethal, hemorrhagic, myotoxic, edema-forming, coagulant, defibrinating and phospholipase A2 activities, showing a similar toxicological profile to the one previously described for B. asper from Costa Rica. In addition, polyvalent antivenoms produced in Mexico and Costa Rica, and currently used in Guatemala, were tested for their ability to neutralize venom’s toxic activities. Both antivenoms were effective against all effects studied, although the Costa Rican product showed higher potency against most activities tested and higher antibody titer against venom components, as determined by enzyme immunoassay. It is suggested that different dosage regimes should be considered when using these antivenoms in B. asper envenomations in Guatemala.
publishDate 2001
dc.date.issued.none.fl_str_mv 2001-02
dc.date.accessioned.none.fl_str_mv 2017-01-27T21:48:12Z
dc.date.available.none.fl_str_mv 2017-01-27T21:48:12Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv http://www.sciencedirect.com/science/article/pii/S0041010100001227
dc.identifier.issn.none.fl_str_mv 0041-0101
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/29467
dc.identifier.doi.none.fl_str_mv 10.1016/S0041-0101(00)00122-7
dc.identifier.codproyecto.none.fl_str_mv 741-89-057
731-99-526
dc.identifier.pmid.none.fl_str_mv 10978760
url http://www.sciencedirect.com/science/article/pii/S0041010100001227
https://hdl.handle.net/10669/29467
identifier_str_mv 0041-0101
10.1016/S0041-0101(00)00122-7
741-89-057
731-99-526
10978760
dc.language.iso.es_ES.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
eu_rights_str_mv embargoedAccess
dc.source.es_ES.fl_str_mv Toxicon; Volumen 39, Número 2-3. 2001
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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Despite its medical relevance, only the venom of Costa Rican populations of this species has been studied to some detail, and there is very little information on intraspecies variability in venom composition and toxicity. Venom of B. asper from Guatemala was analyzed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis, and its basic pharmacological activities were investigated with standard laboratory assays. Venom has lethal, hemorrhagic, myotoxic, edema-forming, coagulant, defibrinating and phospholipase A2 activities, showing a similar toxicological profile to the one previously described for B. asper from Costa Rica. In addition, polyvalent antivenoms produced in Mexico and Costa Rica, and currently used in Guatemala, were tested for their ability to neutralize venom’s toxic activities. Both antivenoms were effective against all effects studied, although the Costa Rican product showed higher potency against most activities tested and higher antibody titer against venom components, as determined by enzyme immunoassay. It is suggested that different dosage regimes should be considered when using these antivenoms in B. asper envenomations in Guatemala.Universidad de Costa Rica/[731-99-526]/UCR/Costa RicaUniversidad de Costa Rica/[741-89-057]/UCR/Costa RicaNetwork for Research and Training in Tropical Diseases in Central America//NeTropica/Consejo Nacional de Ciencia y Tecnología/[Facyt MS-058-1]/CONCYT/GuatemalaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)en_USacceso embargadohttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessToxicon; Volumen 39, Número 2-3. 2001reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRBothrops asperAntivenomNeutralizationGuatemalaSnake venomThe venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenomsartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleTHUMBNAIL167_2001_Toxicon_Saravia_B. asper_Guatemala.pdf.jpg167_2001_Toxicon_Saravia_B. asper_Guatemala.pdf.jpgGenerated Thumbnailimage/jpeg4059https://www.kerwa.ucr.ac.cr/bitstreams/2bc56f0e-3bdd-4af9-bb1c-2b834d2187c2/downloadac1a4e19a52a052c48cca8edae1fa162MD54ORIGINAL167_2001_Toxicon_Saravia_B. asper_Guatemala.pdf167_2001_Toxicon_Saravia_B. asper_Guatemala.pdfapplication/pdf158440https://www.kerwa.ucr.ac.cr/bitstreams/b8732895-fe17-4e63-b97d-bae9dda64c0d/download245aa36afc120dbb2b19e926d79c7664MD51LICENSElicense.txtlicense.txttext/plain; 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