BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY

The aim of the present work was to identify spore-forming Gram-positive Bacillus strains isolated from oceanic waters adjacent to Cuba and to establish a possible relationship between their distribution and the metabolic activity of the isolates. Total protein patterns, derived from SDS-PAGE, were u...

Descripción completa

Detalles Bibliográficos
Autores: Lugioyo-Gallardo, Gladys Margarita, Coto, Orquídea, Álvarez, Carlos, Espinosa, Georgina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Costa Rica
Institución:Universidad Nacional de Costa Rica
Repositorio:Portal de Revistas UNA
Idioma:español
OAI Identifier:oai:www.revistas.una.ac.cr:article/161
Acceso en línea:https://www.revistas.una.ac.cr/index.php/revmar/article/view/161
Access Level:acceso abierto
Palabra clave:Bacillus
distribution
metabolic activity
oceanic waters
Cuba
distribución
actividad metabólica
aguas oceánicas
Distribuição
atividade metabólica
águas oceânicas
Cuba.
id CR_830a03c84e0bdbcc61ba89ffc2ef2e03
oai_identifier_str oai:www.revistas.una.ac.cr:article/161
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.none.fl_str_mv BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
ESPECIES DE BACILLUS EN LAS AGUAS OCEÁNICAS ADYACENTE A CUBA: ASOCIACIÓN ENTRE ELLOS DISTRIBUCIÓN Y ACTIVIDAD METABÓLICA
ESPÉCIES DE BACILLUS NAS ÁGUAS OCEÂNICAS ADJACENTES A CUBA: ASSOCIAÇÃO ENTRE SUA DISTRIBUIÇÃO E ATIVIDADE METABÓLICA
title BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
spellingShingle BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
Lugioyo-Gallardo, Gladys Margarita
Bacillus
distribution
metabolic activity
oceanic waters
Cuba
Bacillus
distribución
actividad metabólica
aguas oceánicas
Cuba
Distribuição
Bacillus
atividade metabólica
águas oceânicas
Cuba.
title_short BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
title_full BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
title_fullStr BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
title_full_unstemmed BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
title_sort BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
dc.creator.none.fl_str_mv Lugioyo-Gallardo, Gladys Margarita
Coto, Orquídea
Álvarez, Carlos
Espinosa, Georgina
author Lugioyo-Gallardo, Gladys Margarita
author_facet Lugioyo-Gallardo, Gladys Margarita
Coto, Orquídea
Álvarez, Carlos
Espinosa, Georgina
author_role author
author2 Coto, Orquídea
Álvarez, Carlos
Espinosa, Georgina
author2_role author
author
author
dc.subject.none.fl_str_mv Bacillus
distribution
metabolic activity
oceanic waters
Cuba
Bacillus
distribución
actividad metabólica
aguas oceánicas
Cuba
Distribuição
Bacillus
atividade metabólica
águas oceânicas
Cuba.
topic Bacillus
distribution
metabolic activity
oceanic waters
Cuba
Bacillus
distribución
actividad metabólica
aguas oceánicas
Cuba
Distribuição
Bacillus
atividade metabólica
águas oceânicas
Cuba.
description The aim of the present work was to identify spore-forming Gram-positive Bacillus strains isolated from oceanic waters adjacent to Cuba and to establish a possible relationship between their distribution and the metabolic activity of the isolates. Total protein patterns, derived from SDS-PAGE, were used to build a non-rooted dendrogram where the strains appeared clustered in three nodes. No direct relationship was observed between a node and particular species. In contrast, according to the physical and chemical characteristics of the zones, node I was different from node II and III, since it comprises strains from the farthest zones of the coast, poorer in nutrients. On the other hand, nodes II and III mainly collect strains isolated from nutrient and organic matter-enriched zones. Associating the node clustering with metabolic activities, it was found that in node I the ratio: number of positive activities/strain was 2.3, followed by node II with a ratio of 3.3, and finally node III exhibiting a ratio equal to 3.7. This could suggest that different total protein patterns in bacteria belonging to the same specie, but coming from environments with different degree of nutrient richness, could be an indicator of the capacities of these microorganisms to adapt and live in different environments.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-11
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Peer-reviewed article
Artículo evaluado por pares
Artigo revisto por pares
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/revmar/article/view/161
10.15359/revmar.2.5
url https://www.revistas.una.ac.cr/index.php/revmar/article/view/161
identifier_str_mv 10.15359/revmar.2.5
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/revmar/article/view/161/120
https://www.revistas.una.ac.cr/index.php/revmar/article/view/161/17522
https://www.revistas.una.ac.cr/index.php/revmar/article/view/161/17523
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/epub+zip
dc.publisher.none.fl_str_mv Universidad Nacional, Costa Rica.
publisher.none.fl_str_mv Universidad Nacional, Costa Rica.
dc.source.none.fl_str_mv Revista Ciencias Marinas y Costeras; Vol. 2 (2010); 61-72
Revista Ciencias Marinas y Costeras; Vol. 2 (2010); 61-72
Revista Ciencias Marinas y Costeras; v. 2 (2010); 61-72
1659-407X
1659-455X
reponame:Portal de Revistas UNA
instname:Universidad Nacional de Costa Rica
instacron:UNA
instname_str Universidad Nacional de Costa Rica
instacron_str UNA
institution UNA
reponame_str Portal de Revistas UNA
collection Portal de Revistas UNA
repository.name.fl_str_mv Portal de Revistas UNA - Universidad Nacional de Costa Rica
repository.mail.fl_str_mv andrea.mora.campos@una.cr
_version_ 1849325817378635776
spelling BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITYESPECIES DE BACILLUS EN LAS AGUAS OCEÁNICAS ADYACENTE A CUBA: ASOCIACIÓN ENTRE ELLOS DISTRIBUCIÓN Y ACTIVIDAD METABÓLICAESPÉCIES DE BACILLUS NAS ÁGUAS OCEÂNICAS ADJACENTES A CUBA: ASSOCIAÇÃO ENTRE SUA DISTRIBUIÇÃO E ATIVIDADE METABÓLICALugioyo-Gallardo, Gladys MargaritaCoto, OrquídeaÁlvarez, CarlosEspinosa, GeorginaBacillusdistributionmetabolic activityoceanic watersCubaBacillusdistribuciónactividad metabólicaaguas oceánicasCubaDistribuiçãoBacillusatividade metabólicaáguas oceânicasCuba.The aim of the present work was to identify spore-forming Gram-positive Bacillus strains isolated from oceanic waters adjacent to Cuba and to establish a possible relationship between their distribution and the metabolic activity of the isolates. Total protein patterns, derived from SDS-PAGE, were used to build a non-rooted dendrogram where the strains appeared clustered in three nodes. No direct relationship was observed between a node and particular species. In contrast, according to the physical and chemical characteristics of the zones, node I was different from node II and III, since it comprises strains from the farthest zones of the coast, poorer in nutrients. On the other hand, nodes II and III mainly collect strains isolated from nutrient and organic matter-enriched zones. Associating the node clustering with metabolic activities, it was found that in node I the ratio: number of positive activities/strain was 2.3, followed by node II with a ratio of 3.3, and finally node III exhibiting a ratio equal to 3.7. This could suggest that different total protein patterns in bacteria belonging to the same specie, but coming from environments with different degree of nutrient richness, could be an indicator of the capacities of these microorganisms to adapt and live in different environments.El objetivo del presente trabajo fue la identificación de cepas de bacilos Gram-positivos esporulados aislados de las aguas oceánicas adyacentes a Cuba y el establecimiento de la posible relación entre la distribución y las actividades metabólicas de los aislados. A partir del patrón de proteínas obtenido mediante electroforesis SDS-PAGE, se construyó un dendrograma no enraizado, lo que permitió la agrupación de las cepas en tres nodos. No se observó una relación directa entre un nodo y especies particulares, sin embargo, se encontraron diferencias entre los nodos al considerar las características físicas y químicas de las zonas; el nodo I fue diferente a los nodos II y III. En ese nodo se agruparon bacterias aisladas de zonas alejadas de la costa, pobres en nutrientes. Por otro lado, los nodos II y III agrupan principalmente a cepas aisladas de aguas más enriquecidas en nutrientes y materia orgánica. Con respecto a la asociación de los aislados en los nodos con las actividades metabólicas, se encontró que la razón entre el número de actividades positivas y el número de aislados fue de 2.3 en el nodo I, seguida del nodo II con 3.3 y por último el nodo III con 3.7. Estos resultados sugieren que, los patrones diferentes de proteínas totales en bacterias que pertenecen a la misma especie, pero que provienen de ambientes con diferente grado de riqueza en nutrientes, podrían ser un indicador de las capacidades de estos microorganismos para adaptarse y vivir en diferentes ambientes.O objetivo do presente trabalho foi a identificação de cepas de bacilos Gram-positivos esporulados isolados das águas oceânicas adjacentes a Cuba e o estabelecimento da possível relação entre a distribuição e as atividades metabólicas dos isolados. A partir do padrão de proteínas obtido mediante eletroforeses SDS-PAGE, construiu-se um dendrograma não enraizado, o que permitiu a agrupação das cepas em três nós. Não se observou uma relação direta entre um nó e espécies particulares, porém, encontraram-se diferenças entre os nós ao considerar as características físicas e químicas das zonas; o nó I foi diferente aos nós II e III. Nesse nó se agruparam bactérias isoladas de zonas distantes da costa, pobres em nutrientes. Por outro lado, os nós II e III agrupam principalmente cepas isoladas de águas mais enriquecidas em nutrientes e matéria orgânica. Com respeito à associação dos isolados nos nós com as atividades metabólicas, encontrou-se que a razão entre o número de atividades positivas e o número de isolados foi de 2.3 no nó I, seguida do nó II com 3.3 e por último o nó III com 3.7. Esses resultados sugerem que, os padrões diferentes de proteínas totais em bactérias que pertencem à mesma espécie, mas que provêm de ambientes com diferente grau de riqueza em nutrientes, poderiam ser um indicador das capacidades desses microrganismos para adaptar-se e viver em diferentes ambientes.Universidad Nacional, Costa Rica.2010-12-11info:eu-repo/semantics/publishedVersionPeer-reviewed articleArtículo evaluado por paresArtigo revisto por paresartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/epub+ziphttps://www.revistas.una.ac.cr/index.php/revmar/article/view/16110.15359/revmar.2.5Revista Ciencias Marinas y Costeras; Vol. 2 (2010); 61-72Revista Ciencias Marinas y Costeras; Vol. 2 (2010); 61-72Revista Ciencias Marinas y Costeras; v. 2 (2010); 61-721659-407X1659-455Xreponame:Portal de Revistas UNAinstname:Universidad Nacional de Costa Ricainstacron:UNAspahttps://www.revistas.una.ac.cr/index.php/revmar/article/view/161/120https://www.revistas.una.ac.cr/index.php/revmar/article/view/161/17522https://www.revistas.una.ac.cr/index.php/revmar/article/view/161/17523acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-03-24T20:20:48Zoai:www.revistas.una.ac.cr:article/161Portal de revistashttp://revistas.una.ac.cr/Universidadhttp://www.una.ac.crhttps://revistas.una.ac.cr/index.php/index/oaiandrea.mora.campos@una.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2022-03-24T20:20:48Portal de Revistas UNA - Universidad Nacional de Costa Ricafalse
score 15,812429

Similares en LA Referencia