Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica.
In order to develop a methodology to evaluate microorganisms capable of solubilizing phosphorus, 54 strains were isolated from a Costa Rican Andisol, and their solubilization potential was quantified under in vitro conditions. For fungi, the solubilization halo in solid medium was used as a selectio...
| Autores: | , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Costa Rica |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/35649 |
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649 |
| Access Level: | acceso abierto |
| Palabra clave: | Phosphorus solubilizing microorganisms phosphorus microbial immobilization microbial innoculants Andisols sustainable agricultural techniques Microorganismos solubilizadores de P inmovilización microbiana de P inoculantes microbianos Andisoles técnicas agrícolas sostenibles |
| id |
CR_5905dfd2db9b262e48d120893b2f4a65 |
|---|---|
| oai_identifier_str |
oai:portal.ucr.ac.cr:article/35649 |
| network_acronym_str |
CR |
| network_name_str |
Costa Rica |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. Aislamiento y evaluación de microorganismos solubilizadores de fósforo de Andisoles de Costa Rica |
| title |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| spellingShingle |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. Vargas Barrantes, Paola Phosphorus solubilizing microorganisms phosphorus microbial immobilization microbial innoculants Andisols sustainable agricultural techniques Microorganismos solubilizadores de P inmovilización microbiana de P inoculantes microbianos Andisoles técnicas agrícolas sostenibles |
| title_short |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| title_full |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| title_fullStr |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| title_full_unstemmed |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| title_sort |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica. |
| dc.creator.none.fl_str_mv |
Vargas Barrantes, Paola Castro Barquero, Leida |
| author |
Vargas Barrantes, Paola |
| author_facet |
Vargas Barrantes, Paola Castro Barquero, Leida |
| author_role |
author |
| author2 |
Castro Barquero, Leida |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
Phosphorus solubilizing microorganisms phosphorus microbial immobilization microbial innoculants Andisols sustainable agricultural techniques Microorganismos solubilizadores de P inmovilización microbiana de P inoculantes microbianos Andisoles técnicas agrícolas sostenibles |
| topic |
Phosphorus solubilizing microorganisms phosphorus microbial immobilization microbial innoculants Andisols sustainable agricultural techniques Microorganismos solubilizadores de P inmovilización microbiana de P inoculantes microbianos Andisoles técnicas agrícolas sostenibles |
| description |
In order to develop a methodology to evaluate microorganisms capable of solubilizing phosphorus, 54 strains were isolated from a Costa Rican Andisol, and their solubilization potential was quantified under in vitro conditions. For fungi, the solubilization halo in solid medium was used as a selection criteria, and for bacteria and yeasts, pH and P in solution in liquid medium were the criteria usedfor selection. As for the in vitro manipulation, fungi showed greater stability to preserve their ability to solubilize P. Subsequently, two trials were conducted in greenhouse using Andisol soil and rice as an indicator plant. In the first trial, the best microorganisms evaluated in vitro were compared, namely Aspergillus flavus, Pseudomonas fragi, Pichia anomala and an absolute control. Plants were harvested at 100 days, the treatment with A. flavus increased dry weight of the root, but the root/stem ratio was less than 1 and the foliar P was not increased (0.13%). However, in the soil of the A. flavus treatment, population of solubilizers was increased 100-fold and soluble P rose by 2.67 mg.l-1 over the control without plants showing any benefit, probably because P in the soil solution did not increase enough due the property of Andisols to retain this element. Due to the ineffectiveness of bacteria and yeast evaluated, in the second trial, an attempt was made to increase the amount of foliar P by individually inoculating the following fungi: Aspergillus flavus, Cladosporium sp., Penicillium sp., Trichoderma longibrachiatum, together with phosphate rock (RF). With the addition of RF, the inoculated fungi increased root fresh weight up to 131%, the R/T ratio in fresh showed values higher than 1 and the foliar P concentration increased by 13% compared to the control with RF. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12-17 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion Article Artículo artículo original http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649 10.15517/rac.v43i1.35649 |
| url |
https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649 |
| identifier_str_mv |
10.15517/rac.v43i1.35649 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36271 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36344 https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36597 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2018 Agronomía Costarricense https://creativecommons.org/licenses/by-nc-nd/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2018 Agronomía Costarricense https://creativecommons.org/licenses/by-nc-nd/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/epub+zip text/html |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Agronomía Costarricense; Agronomía Costarricense: Vol. 43, Issue 1 (January-June) Agronomía Costarricense; Agronomía Costarricense: Vol. 43, Nº 1 (Enero-Junio) 2215-2202 0377-9424 10.15517/rac.v43i1 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
| instname_str |
Universidad de Costa Rica |
| instacron_str |
UCR |
| institution |
UCR |
| reponame_str |
Portal de Revistas UCR |
| collection |
Portal de Revistas UCR |
| repository.name.fl_str_mv |
Portal de Revistas UCR - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
jorge.polanco@ucr.ac.cr |
| _version_ |
1849325422179778560 |
| spelling |
Isolation and evaluation of phosphorus solubilizing microorganisms from Andisol soils in Costa Rica.Aislamiento y evaluación de microorganismos solubilizadores de fósforo de Andisoles de Costa RicaVargas Barrantes, PaolaCastro Barquero, LeidaPhosphorus solubilizing microorganismsphosphorus microbial immobilization microbial innoculantsAndisolssustainable agricultural techniquesMicroorganismos solubilizadores de Pinmovilización microbiana de Pinoculantes microbianosAndisolestécnicas agrícolas sosteniblesIn order to develop a methodology to evaluate microorganisms capable of solubilizing phosphorus, 54 strains were isolated from a Costa Rican Andisol, and their solubilization potential was quantified under in vitro conditions. For fungi, the solubilization halo in solid medium was used as a selection criteria, and for bacteria and yeasts, pH and P in solution in liquid medium were the criteria usedfor selection. As for the in vitro manipulation, fungi showed greater stability to preserve their ability to solubilize P. Subsequently, two trials were conducted in greenhouse using Andisol soil and rice as an indicator plant. In the first trial, the best microorganisms evaluated in vitro were compared, namely Aspergillus flavus, Pseudomonas fragi, Pichia anomala and an absolute control. Plants were harvested at 100 days, the treatment with A. flavus increased dry weight of the root, but the root/stem ratio was less than 1 and the foliar P was not increased (0.13%). However, in the soil of the A. flavus treatment, population of solubilizers was increased 100-fold and soluble P rose by 2.67 mg.l-1 over the control without plants showing any benefit, probably because P in the soil solution did not increase enough due the property of Andisols to retain this element. Due to the ineffectiveness of bacteria and yeast evaluated, in the second trial, an attempt was made to increase the amount of foliar P by individually inoculating the following fungi: Aspergillus flavus, Cladosporium sp., Penicillium sp., Trichoderma longibrachiatum, together with phosphate rock (RF). With the addition of RF, the inoculated fungi increased root fresh weight up to 131%, the R/T ratio in fresh showed values higher than 1 and the foliar P concentration increased by 13% compared to the control with RF.Con el objetivo de desarrollar una metodología para evaluar microorganismos capaces de solubilizar fósforo, se aislaron 54 cepas de un Andisol de Costa Rica, y se cuantificó su potencial de solubilización in vitro. Para los hongos se empleó como criterio de selección el halo de solubilización en medio sólido y para las bacterias y levaduras el pH y el P en solución, en medio líquido. Ante la manipulación in vitro, los hongos presentaron mayor estabilidad para conservar su habilidad para solubilizar P. Posteriormente, se realizaron dos ensayos en invernadero utilizando el Andisol y arroz como planta indicadora. En el primer ensayo se compararon los mejores microorganismos evaluados in vitro, a saber Aspergillus flavus, Pseudomonas fragi, Pichia anomala y un testigo absoluto. Las plantas se cosecharon a los 100 días, el tratamiento con A. flavus aumentó el peso seco de la raíz, pero la relación raíz/tallo fue menor a 1 y no se incrementó el P foliar (0,13%). Sin embargo, en el suelo del tratamiento con A. flavus se incrementó 100 veces la población de solubilizadores y el P soluble se elevó en 2,67 mg.l-1 respecto al testigo sin que las plantas mostraran beneficio, probablemente debido a que el P en la solución del suelo no aumentó lo suficiente por la propiedad de los Andisoles de retener el elemento. Debido a la inefectividad de la bacteria y levadura evaluadas, en el segundo ensayo, se trató de incrementar la cantidad de P foliar inoculando de manera individual los siguientes hongos: Aspergillus flavus, Cladosporium sp., Penicillium sp., Trichoderma longibrachiatum, junto con roca fosfórica (RF). Con la adición de RF, los hongos inoculados incrementaron el peso fresco radical hasta en 131%, la relación R/T en fresco mostró valores superiores a 1 y se incrementó la concentración del P foliar hasta en 13% respecto al testigo con RF.Universidad de Costa Rica2018-12-17info:eu-repo/semantics/publishedVersionArticleArtículoartículo originalhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfapplication/epub+ziptext/htmlhttps://revistas.ucr.ac.cr/index.php/agrocost/article/view/3564910.15517/rac.v43i1.35649Agronomía Costarricense; Agronomía Costarricense: Vol. 43, Issue 1 (January-June)Agronomía Costarricense; Agronomía Costarricense: Vol. 43, Nº 1 (Enero-Junio)2215-22020377-942410.15517/rac.v43i1reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspahttps://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36271https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36344https://revistas.ucr.ac.cr/index.php/agrocost/article/view/35649/36597Derechos de autor 2018 Agronomía Costarricensehttps://creativecommons.org/licenses/by-nc-nd/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2024-08-30T15:42:59Zoai:portal.ucr.ac.cr:article/35649Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:34:07.518Portal de Revistas UCR - Universidad de Costa Ricafalse |
| score |
15,812429 |