Isolation of a Toxigenic and Clinical Genotype of Clostridium difficile in Retail Meats in Costa Rica

We isolated a regional toxigenic genotype of Clostridium difficile, previously found in human infection in 4 of 200 (2%) samples of retail meats for human consumption: 1 of 67 samples of beef, 2 of 66 of pork, and 1 of 67 of poultry meat. These four isolates were positive for the tcdA and tcdB genes...

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Detalles Bibliográficos
Autores: Quesada Gómez, Carlos, Mulvey, Michael R., Vargas Dengo, Pablo Antonio, Gamboa Coronado, María del Mar, Rodríguez Sánchez, César, Rodríguez Cavallini, Evelyn
Tipo de recurso: artículo
Fecha de publicación:2013
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/75160
Acceso en línea:http://jfoodprotection.org/doi/abs/10.4315/0362-028X.JFP-12-169?code=fopr-site
https://hdl.handle.net/10669/75160
Access Level:acceso embargado
Palabra clave:Clostridium
Carnes
Costa Rica
589.957 286 Clostridium
Descripción
Sumario:We isolated a regional toxigenic genotype of Clostridium difficile, previously found in human infection in 4 of 200 (2%) samples of retail meats for human consumption: 1 of 67 samples of beef, 2 of 66 of pork, and 1 of 67 of poultry meat. These four isolates were positive for the tcdA and tcdB genes but negative for deletion of the tcdC and cdtB genes. All strains induced cytopathic effects in HeLa cells. However, they were susceptible to some antibiotics to which clinical isolates are often resistant. All strains were susceptible to vancomycin, metronidazole, moxifloxacin, and rifampicin but resistant to clindamycin and ciprofloxacin. This first report of isolation of C. difficile in foodstuff from Latin America lends support to the notion that animal products serve as a reservoir for clinical strains of this pathogen in the community.