Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants

The purpose of this in vitro study was to develop a polymeric nanofiber mesh coating for titanium implant surfaces and assess its contribution to the cellular response. Two types of dental implants TiUltraTM and TiUniteTM (Nobel Biocare) were coated with poly (lactic acid) nanofibers fabricated usin...

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Autores: Vázquez-Vázquez, Febe Carolina, Arenas-Alatorre, Jesús, Chavarría-Bolaños, Daniel, Pozos-Guillén, Amaury, Alvárez-Pérez, Marco Antonio, Ortiz-Magdaleno, Marine
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/61225
Acceso en línea:https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225
Access Level:acceso abierto
Palabra clave:Nanofibers; Osteoblasts; Cell response; Titanium dental implant; Surface; Poly (lactic acid).
Nanofibras; Osteoblastos; Respuesta celular; Implantes dentales de titanio; Superficie; Ácido poliláctico.
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oai_identifier_str oai:portal.ucr.ac.cr:article/61225
network_acronym_str CR
network_name_str Costa Rica
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dc.title.none.fl_str_mv Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
Respuesta celular de la superficie funcionalizada de un recubrimiento nanofibrilar polimérico en implantes dentales de titanio
title Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
spellingShingle Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
Vázquez-Vázquez, Febe Carolina
Nanofibers; Osteoblasts; Cell response; Titanium dental implant; Surface; Poly (lactic acid).
Nanofibras; Osteoblastos; Respuesta celular; Implantes dentales de titanio; Superficie; Ácido poliláctico.
title_short Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
title_full Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
title_fullStr Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
title_full_unstemmed Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
title_sort Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium Implants
dc.creator.none.fl_str_mv Vázquez-Vázquez, Febe Carolina
Arenas-Alatorre, Jesús
Chavarría-Bolaños, Daniel
Pozos-Guillén, Amaury
Alvárez-Pérez, Marco Antonio
Ortiz-Magdaleno, Marine
author Vázquez-Vázquez, Febe Carolina
author_facet Vázquez-Vázquez, Febe Carolina
Arenas-Alatorre, Jesús
Chavarría-Bolaños, Daniel
Pozos-Guillén, Amaury
Alvárez-Pérez, Marco Antonio
Ortiz-Magdaleno, Marine
author_role author
author2 Arenas-Alatorre, Jesús
Chavarría-Bolaños, Daniel
Pozos-Guillén, Amaury
Alvárez-Pérez, Marco Antonio
Ortiz-Magdaleno, Marine
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nanofibers; Osteoblasts; Cell response; Titanium dental implant; Surface; Poly (lactic acid).
Nanofibras; Osteoblastos; Respuesta celular; Implantes dentales de titanio; Superficie; Ácido poliláctico.
topic Nanofibers; Osteoblasts; Cell response; Titanium dental implant; Surface; Poly (lactic acid).
Nanofibras; Osteoblastos; Respuesta celular; Implantes dentales de titanio; Superficie; Ácido poliláctico.
description The purpose of this in vitro study was to develop a polymeric nanofiber mesh coating for titanium implant surfaces and assess its contribution to the cellular response. Two types of dental implants TiUltraTM and TiUniteTM (Nobel Biocare) were coated with poly (lactic acid) nanofibers fabricated using the air-jet spinning technique (AJS). The morphology of the polymeric nanofibers was characterized by scanning electron micros­copy (SEM), and the biocompatibility was evaluated in terms of cell adhesion by using human fetal osteoblasts (hFOB). The cellular localization was observed under a fluorescent microscope, and the gene expression of Col 1, ALP, and OCN was evaluated by RT-PCR. The micrographs showed that the polymeric nanofiber coated the titaium (Ti) dental implant surfaces with a randomized deposition anchored between the depth of the threads, and well-interconnected pores were observed. Cell adhesion increased significantly (P<.05) more on the surfaces of Ti dental implants coated with the polymeric nanofiber mesh than on those not coated. After 21 days, the cell adhesion decreased significatively on the uncoated surfaces (P<.05). Col 1 and ALP genes showed a higher level of expression on dental implant surfaces coated with polymeric nanofibers mesh than on uncoated surfaces. Coating Ti dental implant surfaces with polymeric nanofiber mesh is a straightforward deposition technique for stimulating the cell response and improving the gene expression of osteogenic markers.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-30
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225
10.15517/ijds.2024.61225
url https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225
identifier_str_mv 10.15517/ijds.2024.61225
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60748
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60749
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60750
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/epub+zip
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Odovtos - International Journal of Dental Sciences; Vol. 26 No. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 186-196
Odovtos International Journal of Dental Sciences; Vol. 26 Núm. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 186-196
2215-3411
1659-1046
10.15517/ijds.2024
reponame:Portal de Revistas UCR
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Cellular Response of Surface Functionalized Polymeric Fiber Mesh Coating Onto Dental Titanium ImplantsRespuesta celular de la superficie funcionalizada de un recubrimiento nanofibrilar polimérico en implantes dentales de titanioVázquez-Vázquez, Febe CarolinaArenas-Alatorre, JesúsChavarría-Bolaños, DanielPozos-Guillén, AmauryAlvárez-Pérez, Marco AntonioOrtiz-Magdaleno, MarineNanofibers; Osteoblasts; Cell response; Titanium dental implant; Surface; Poly (lactic acid).Nanofibras; Osteoblastos; Respuesta celular; Implantes dentales de titanio; Superficie; Ácido poliláctico.The purpose of this in vitro study was to develop a polymeric nanofiber mesh coating for titanium implant surfaces and assess its contribution to the cellular response. Two types of dental implants TiUltraTM and TiUniteTM (Nobel Biocare) were coated with poly (lactic acid) nanofibers fabricated using the air-jet spinning technique (AJS). The morphology of the polymeric nanofibers was characterized by scanning electron micros­copy (SEM), and the biocompatibility was evaluated in terms of cell adhesion by using human fetal osteoblasts (hFOB). The cellular localization was observed under a fluorescent microscope, and the gene expression of Col 1, ALP, and OCN was evaluated by RT-PCR. The micrographs showed that the polymeric nanofiber coated the titaium (Ti) dental implant surfaces with a randomized deposition anchored between the depth of the threads, and well-interconnected pores were observed. Cell adhesion increased significantly (P<.05) more on the surfaces of Ti dental implants coated with the polymeric nanofiber mesh than on those not coated. After 21 days, the cell adhesion decreased significatively on the uncoated surfaces (P<.05). Col 1 and ALP genes showed a higher level of expression on dental implant surfaces coated with polymeric nanofibers mesh than on uncoated surfaces. Coating Ti dental implant surfaces with polymeric nanofiber mesh is a straightforward deposition technique for stimulating the cell response and improving the gene expression of osteogenic markers. El objetivo de este estudio in vitro fue realizar un recubrimiento con nanofibras poliméricas para superficies de implantes de titanio y evaluar el comportamiento de la respuesta celular. Se recubrieron dos tipos de implantes dentales TiUltraTM y TiUniteTM (Nobel Biocare) con nanofibras de ácido poliláctico (PLA) fabricadas con la técnica de hilado por propulsión de aire (AJS). Se caracterizó la morfología de las nanofibras poliméricas por Microscopia Electronica de Barrido (MEB) y se evaluó la biocompatibilidad en términos de adhesión celular utilizando osteoblastos fetales humanos (hFOB). La localización celular se observó con el microscopio fluorescente y la expresión génica de Col 1, ALP y OCN se evaluó con RT-PCR. Las micrografías mostraron que las nanofibras poliméricas recubrieron las superficies de los implantes dentales de titanio (Ti) con una deposición aleatoria lo que generó poros interconectados fibrilares. La adhesión celular aumentó significativamente (P<.05) en las superficies de los implantes dentales de Ti recubiertas con las nanofibras poliméricas comparado con las no recubiertas. Después de 21 días, la adhesión celular disminuyó significativamente en las superficies no recubiertas (P<.05). Los genes Col 1 y ALP mostraron un mayor nivel de expresión en las superficies de los implantes dentales recubiertas con nanofibras poliméricas comparado con las no recubiertas. El recubrimiento de superficies de implantes dentales de Ti con nanofibras poliméricas es una técnica de deposición sencilla para estimular la respuesta celular y mejorar la expresión génica de marcadores osteogénicos. Universidad de Costa Rica2024-08-30info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/epub+ziphttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/6122510.15517/ijds.2024.61225Odovtos - International Journal of Dental Sciences; Vol. 26 No. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 186-196Odovtos International Journal of Dental Sciences; Vol. 26 Núm. 3 (2024): (September-December) Odovtos-International Journal of Dental Sciences; 186-1962215-34111659-104610.15517/ijds.2024reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60748https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60749https://revistas.ucr.ac.cr/index.php/Odontos/article/view/61225/60750Derechos de autor 2024 Febe Carolina Vázquez-Vázquez, Jesús Arenas-Alatorre, Daniel Chavarría-Bolaños, Amaury Pozos-Guillén, Marco Antonio Alvárez-Pérez, Marine Ortiz-Magdalenohttps://creativecommons.org/licenses/by-nc-sa/4.0/acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2025-01-17T19:11:10Zoai:portal.ucr.ac.cr:article/61225Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:44:46.653Portal de Revistas UCR - Universidad de Costa Ricafalse
score 15.812455

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