Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803

Synechocystis sp. PCC 6803, a freshwater cyanobacterium, was cultivated outdoors in a 50 L tubular photobioreactor to study the acclimation of cells to solar light in cultures grown at different initial mean biomass concentrations (1.10, 0.60, and 0.32 g L− 1). Chlorophyll fluorescence quenching ana...

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Detalles Bibliográficos
Autores: Touloupakis, Eleftherios, Silva Benavides, Ana Margarita, Cicchi, Bernardo, Torzillo, Giuseppe
Tipo de recurso: artículo
Fecha de publicación:2016
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/73435
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2211926416301990?via%3Dihub
https://hdl.handle.net/10669/73435
Access Level:acceso embargado
Palabra clave:Synechocystis sp. PCC 6803
Photobioreactor
Fluorescence
Biohydrogen
Carbohydrates
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dc.title.es_ES.fl_str_mv Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
title Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
spellingShingle Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
Touloupakis, Eleftherios
Synechocystis sp. PCC 6803
Photobioreactor
Fluorescence
Biohydrogen
Carbohydrates
title_short Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
title_full Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
title_fullStr Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
title_full_unstemmed Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
title_sort Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803
dc.creator.none.fl_str_mv Touloupakis, Eleftherios
Silva Benavides, Ana Margarita
Cicchi, Bernardo
Torzillo, Giuseppe
author Touloupakis, Eleftherios
author_facet Touloupakis, Eleftherios
Silva Benavides, Ana Margarita
Cicchi, Bernardo
Torzillo, Giuseppe
author_role author
author2 Silva Benavides, Ana Margarita
Cicchi, Bernardo
Torzillo, Giuseppe
author2_role author
author
author
dc.subject.es_ES.fl_str_mv Synechocystis sp. PCC 6803
Photobioreactor
Fluorescence
Biohydrogen
Carbohydrates
topic Synechocystis sp. PCC 6803
Photobioreactor
Fluorescence
Biohydrogen
Carbohydrates
description Synechocystis sp. PCC 6803, a freshwater cyanobacterium, was cultivated outdoors in a 50 L tubular photobioreactor to study the acclimation of cells to solar light in cultures grown at different initial mean biomass concentrations (1.10, 0.60, and 0.32 g L− 1). Chlorophyll fluorescence quenching analysis, coupled with measurements of photosynthesis and respiration rates, showed that photoinhibition strongly affected the performance of the culture grown at lowest biomass concentration (0.32 g L− 1). In this culture the Fv/Fm ratio at noon declined to one fourth compared to the morning, and no recovery occurred in the afternoon. Optimal biomass concentration for growth was 0.60 g L− 1 which resulted in a biomass yield of 24.6 g m− 2 day− 1. The ability of the cells to produce hydrogen via an indirect light driven process was investigated both in the laboratory and in the outdoor photobioreactor. The rate of hydrogen production achieved in the laboratory reached 3.07 mL H2 L− 1 h− 1 (i.e., 6.55 μmol H2 mg Chl− 1 h− 1) while that attained in the 50 L photobioreactor was 0.05 mL H2 L− 1 h− 1 (0.39 μmol H2 mg Chl− 1 h− 1). The production of hydrogen in the dark was sustained by fermentation of carbohydrates accumulated during the nitrogen starvation of cells under the light.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2017-11-02T20:00:51Z
dc.date.available.none.fl_str_mv 2017-11-02T20:00:51Z
dc.type.none.fl_str_mv artículo original
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info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv http://www.sciencedirect.com/science/article/pii/S2211926416301990?via%3Dihub
dc.identifier.issn.none.fl_str_mv 2211-9264
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/73435
dc.identifier.doi.none.fl_str_mv 10.1016/j.algal.2016.06.010
url http://www.sciencedirect.com/science/article/pii/S2211926416301990?via%3Dihub
https://hdl.handle.net/10669/73435
identifier_str_mv 2211-9264
10.1016/j.algal.2016.06.010
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dc.rights.none.fl_str_mv acceso embargado
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rights_invalid_str_mv acceso embargado
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dc.source.es_ES.fl_str_mv Algal Research; Volumen 18. 2016
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spelling Touloupakis, Eleftheriosa75b8cca-3adf-4795-85a1-0491bc8f572eSilva Benavides, Ana Margarita50380000-a61b-40c8-9344-87e295007a96600Cicchi, Bernardo8d79157f-8306-4a81-ab67-a9507073842fTorzillo, Giuseppe58768475-c661-43ec-beaa-900eec9796646002017-11-02T20:00:51Z2017-11-02T20:00:51Z2016http://www.sciencedirect.com/science/article/pii/S2211926416301990?via%3Dihub2211-9264https://hdl.handle.net/10669/7343510.1016/j.algal.2016.06.010Synechocystis sp. PCC 6803, a freshwater cyanobacterium, was cultivated outdoors in a 50 L tubular photobioreactor to study the acclimation of cells to solar light in cultures grown at different initial mean biomass concentrations (1.10, 0.60, and 0.32 g L− 1). Chlorophyll fluorescence quenching analysis, coupled with measurements of photosynthesis and respiration rates, showed that photoinhibition strongly affected the performance of the culture grown at lowest biomass concentration (0.32 g L− 1). In this culture the Fv/Fm ratio at noon declined to one fourth compared to the morning, and no recovery occurred in the afternoon. Optimal biomass concentration for growth was 0.60 g L− 1 which resulted in a biomass yield of 24.6 g m− 2 day− 1. The ability of the cells to produce hydrogen via an indirect light driven process was investigated both in the laboratory and in the outdoor photobioreactor. The rate of hydrogen production achieved in the laboratory reached 3.07 mL H2 L− 1 h− 1 (i.e., 6.55 μmol H2 mg Chl− 1 h− 1) while that attained in the 50 L photobioreactor was 0.05 mL H2 L− 1 h− 1 (0.39 μmol H2 mg Chl− 1 h− 1). The production of hydrogen in the dark was sustained by fermentation of carbohydrates accumulated during the nitrogen starvation of cells under the light.European Union Seventh Framework Programme/[308518]/FP7/EuropaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencias del Mar y Limnología (CIMAR)UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaen_USacceso embargadohttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessAlgal Research; Volumen 18. 2016reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRSynechocystis sp. 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