Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study

Current research highlighted the importance to recognize feasible biomarkers for early diagnoses and treatment in oral cancer. Our study analyzed the expression and spatial distribution of ALDH1A1, FGFR2, caspase-3, and CD44 in Oral Squamous Cell Carcinoma (OSCC) and leukoplakia with and without ora...

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Autores: Boza Oreamuno, Yadira V., Reyes-Carmona, Jessie F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/50369
Acceso en línea:https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369
Access Level:acceso abierto
Palabra clave:Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.
Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.
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network_name_str Costa Rica
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dc.title.none.fl_str_mv Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
Inmunoexpresión de ALDH1A1, FGFR2, CD44 y Caspasa-3 en Carcinoma Oral de Células Escamosas y Leucoplasias: un estudio piloto
title Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
spellingShingle Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
Boza Oreamuno, Yadira V.
Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.
Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.
title_short Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
title_full Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
title_fullStr Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
title_full_unstemmed Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
title_sort Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot Study
dc.creator.none.fl_str_mv Boza Oreamuno, Yadira V.
Reyes-Carmona, Jessie F.
author Boza Oreamuno, Yadira V.
author_facet Boza Oreamuno, Yadira V.
Reyes-Carmona, Jessie F.
author_role author
author2 Reyes-Carmona, Jessie F.
author2_role author
dc.subject.none.fl_str_mv Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.
Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.
topic Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.
Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.
description Current research highlighted the importance to recognize feasible biomarkers for early diagnoses and treatment in oral cancer. Our study analyzed the expression and spatial distribution of ALDH1A1, FGFR2, caspase-3, and CD44 in Oral Squamous Cell Carcinoma (OSCC) and leukoplakia with and without oral mucosal dysplasia. Paraffin-embedded samples of OSCC (n=5), leukoplakia with (n=5) and without (n=5) dysplasia obtained by incisional biopsies were processed using conventional histochemical techniques. Immunohistochemistry was performed using antibodies against ALDH1A1, FGFR2, caspase-3, and CD44. Images of the immunohistochemically stained tissue sections were analyzed according to the intensity of the immunostaining of each marker and classified in Scores. The Kruskal-Wallis test was performed (p≤0.05). Our results demonstrated a statically difference in the expression of all immunomarkers between OSCC and leukoplakia without dysplasia, being more significant in FGFR2 and ALDH1A1. Within the limitations of this study, our data showed that all biomarkers were overexpressed in OSCC and leukoplakia with oral mucosa dysplasia, suggesting that the presence of dysplasia is a significant clinic-pathologic predictor for malignant transformation.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-26
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369
10.15517/ijds.2022.50369
url https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369
identifier_str_mv 10.15517/ijds.2022.50369
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50297
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50298
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50299
dc.rights.none.fl_str_mv Derechos de autor 2022 CC-BY-NC-SA 4.0
https://creativecommons.org/licenses/by-nc-sa/4.0
acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2022 CC-BY-NC-SA 4.0
https://creativecommons.org/licenses/by-nc-sa/4.0
acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/epub+zip
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Odovtos - International Journal of Dental Sciences; Vol. 25 No. 2 (2023): (May-August) ODOVTOS-International Journal of Dental Sciences; 103-111
Odovtos International Journal of Dental Sciences; Vol. 25 Núm. 2 (2023): (May-August) ODOVTOS-International Journal of Dental Sciences; 103-111
2215-3411
1659-1046
10.15517/ijds.2023
reponame:Portal de Revistas UCR
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instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous Cell Carcinoma and Leukoplakia: A Pilot StudyInmunoexpresión de ALDH1A1, FGFR2, CD44 y Caspasa-3 en Carcinoma Oral de Células Escamosas y Leucoplasias: un estudio pilotoBoza Oreamuno, Yadira V.Reyes-Carmona, Jessie F.Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.Current research highlighted the importance to recognize feasible biomarkers for early diagnoses and treatment in oral cancer. Our study analyzed the expression and spatial distribution of ALDH1A1, FGFR2, caspase-3, and CD44 in Oral Squamous Cell Carcinoma (OSCC) and leukoplakia with and without oral mucosal dysplasia. Paraffin-embedded samples of OSCC (n=5), leukoplakia with (n=5) and without (n=5) dysplasia obtained by incisional biopsies were processed using conventional histochemical techniques. Immunohistochemistry was performed using antibodies against ALDH1A1, FGFR2, caspase-3, and CD44. Images of the immunohistochemically stained tissue sections were analyzed according to the intensity of the immunostaining of each marker and classified in Scores. The Kruskal-Wallis test was performed (p≤0.05). Our results demonstrated a statically difference in the expression of all immunomarkers between OSCC and leukoplakia without dysplasia, being more significant in FGFR2 and ALDH1A1. Within the limitations of this study, our data showed that all biomarkers were overexpressed in OSCC and leukoplakia with oral mucosa dysplasia, suggesting that the presence of dysplasia is a significant clinic-pathologic predictor for malignant transformation. La actual evidencia científica enfatiza la importancia de reconocer biomarcadores viables para el diagnóstico y tratamiento temprano del cáncer oral. Nuestro estudio piloto analizó la expresión y distribución espacial de ALDH1A1, FGFR2, caspasa-3 y CD44 en carcinoma oral de células escamosas (COCE) y en leucoplasia con o sin displasia de la mucosa oral. Las muestras incluidas en parafina de COCE (n=5), con (n=5) y sin (n=5) displasia fueron obtenidas mediante biopsias incisionales, las cuales se procesaron utilizando técnicas histoquímicas convencionales. El análisis inmunohistoquímico se realizó utilizando anticuerpos contra ALDH1A1, FGFR2, caspasa-3 y CD44. Las imágenes de las secciones de cada muestra fueron analizadas según la intensidad de inmunoexpresión de cada marcador y se clasificaron en diferentes escalas (scores). Se realizó la prueba de Kruskal-Wallis (valores de p<0,05). Nuestros resultados demostraron una diferencia estadística en la expresión de todos los inmunomarcadores entre COCE y las muestras con leucoplasia sin displasia, siendo más significativa en FGFR2 y ALDH1A1. Considerando las limitaciones de este estudio, los datos sugieren que la presencia de displasia en la mucosa oral es un importante predictor clínico-patológico de transformación maligna.Universidad de Costa Rica2023-04-26info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/epub+ziphttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/5036910.15517/ijds.2022.50369Odovtos - International Journal of Dental Sciences; Vol. 25 No. 2 (2023): (May-August) ODOVTOS-International Journal of Dental Sciences; 103-111Odovtos International Journal of Dental Sciences; Vol. 25 Núm. 2 (2023): (May-August) ODOVTOS-International Journal of Dental Sciences; 103-1112215-34111659-104610.15517/ijds.2023reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50297https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50298https://revistas.ucr.ac.cr/index.php/Odontos/article/view/50369/50299Derechos de autor 2022 CC-BY-NC-SA 4.0https://creativecommons.org/licenses/by-nc-sa/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2023-04-26T19:50:45Zoai:portal.ucr.ac.cr:article/50369Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:44:27.523Portal de Revistas UCR - Universidad de Costa Ricafalse
score 15,811543

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