Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)

Bothriechis is a genus of eleven currently recognized slender and arboreal venomous snakes, commonly called palm-pitvipers that range from southern Mexico to northern South America. Despite dietary studies suggesting that palm-pitvipers are generalists with an ontogenetic shift toward endothermic pr...

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Detalles Bibliográficos
Autores: Pla Ferrer, Davinia, Sanz, Libia, Sasa Marín, Mahmood, Acevedo, Manuel E., Dwyer, Quetzal, Durban, Jordi, Pérez, Alicia, Rodríguez, Yania, Lomonte, Bruno, Calvete Chornet, Juan José
Tipo de recurso: artículo
Fecha de publicación:2017
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/74866
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S1874391916304444?via%3Dihub#!
https://hdl.handle.net/10669/74866
Access Level:acceso embargado
Palabra clave:Venomics
Genus Bothriechis
Arboreal palm-pitvipers
Bothriechis bicolor
Bothriechis aurife
Bothriechis thalassinus
Bothriechis marchi
Neonate B. schlegelii
Neonate B. lateralis
Snake venom
571.95 Toxicología
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oai_identifier_str oai:kerwa.ucr.ac.cr:10669/74866
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.es_ES.fl_str_mv Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
title Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
spellingShingle Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
Pla Ferrer, Davinia
Venomics
Genus Bothriechis
Arboreal palm-pitvipers
Bothriechis bicolor
Bothriechis aurife
Bothriechis thalassinus
Bothriechis marchi
Neonate B. schlegelii
Neonate B. lateralis
Snake venom
571.95 Toxicología
title_short Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
title_full Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
title_fullStr Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
title_full_unstemmed Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
title_sort Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
dc.creator.none.fl_str_mv Pla Ferrer, Davinia
Sanz, Libia
Sasa Marín, Mahmood
Acevedo, Manuel E.
Dwyer, Quetzal
Durban, Jordi
Pérez, Alicia
Rodríguez, Yania
Lomonte, Bruno
Calvete Chornet, Juan José
author Pla Ferrer, Davinia
author_facet Pla Ferrer, Davinia
Sanz, Libia
Sasa Marín, Mahmood
Acevedo, Manuel E.
Dwyer, Quetzal
Durban, Jordi
Pérez, Alicia
Rodríguez, Yania
Lomonte, Bruno
Calvete Chornet, Juan José
author_role author
author2 Sanz, Libia
Sasa Marín, Mahmood
Acevedo, Manuel E.
Dwyer, Quetzal
Durban, Jordi
Pérez, Alicia
Rodríguez, Yania
Lomonte, Bruno
Calvete Chornet, Juan José
author2_role author
author
author
author
author
author
author
author
author
dc.subject.es_ES.fl_str_mv Venomics
Genus Bothriechis
Arboreal palm-pitvipers
Bothriechis bicolor
Bothriechis aurife
Bothriechis thalassinus
Bothriechis marchi
Neonate B. schlegelii
Neonate B. lateralis
Snake venom
571.95 Toxicología
topic Venomics
Genus Bothriechis
Arboreal palm-pitvipers
Bothriechis bicolor
Bothriechis aurife
Bothriechis thalassinus
Bothriechis marchi
Neonate B. schlegelii
Neonate B. lateralis
Snake venom
571.95 Toxicología
description Bothriechis is a genus of eleven currently recognized slender and arboreal venomous snakes, commonly called palm-pitvipers that range from southern Mexico to northern South America. Despite dietary studies suggesting that palm-pitvipers are generalists with an ontogenetic shift toward endothermic prey, venom proteomic analyses have revealed remarkable divergence between the venoms of the Costa Rican species, B. lateralis, B. schlegelii, B. supraciliaris, and B. nigroviridis. To achieve a more complete picture of the venomic landscape across Bothriechis, the venom proteomes of biodiversity of the northern Middle American highland palm-pitvipers, B. thalassinus, B. aurifer, andB. bicolor fromGuatemala, B. marchi fromHonduras, and neonate Costa Rican B. lateralis and B. schlegelii, were investigated. B. thalassinus and B. aurifer venoms are comprised by similar toxin arsenals dominated by SVMPs (33–39% of the venom proteome), CTLs (11–16%), BPP-like molecules (10–13%), and CRISPs (5–10%), and are characterized by the absence of PLA2 proteins. Conversely, the predominant (35%) components of B. bicolor are D49-PLA2 molecules. The venom proteome of B. marchi is similar to B. aurifer and B. thalassinus in that it is rich in SVMPs and BPPs, but also contains appreciable amounts (14.3%) of PLA2s. The major toxin family found in the venoms of both neonate B. lateralis and B. schlegelii, is serine proteinase (SVSP), comprising about 20% of their toxin arsenals. The venomof neonate B. schlegelii is the only palm-pitviper venom where relative high amounts of Kunitz-type (6.3%) and γPLA2 (5.2%) inhibitors have been identified. Despite notable differences between their proteomes, neonate venoms are more similar to each other than to adults of their respective species. However, the ontogenetic changes taking place in the venom of B. lateralis strongly differ from those that occur in the venom of B. schlegelii. Thus, the ontogenetic change in B. lateralis produces a SVMP-rich venom, whereas in B. schlegelii the age-dependent compositional shift generates a PLA2-rich venom. Overall, genus-wide venomics illustrate the high evolvability of palm-pitviper venoms. The integration of the pattern of venom variation across Bothriechis into a phylogenetic and biogeographic framework may lay the foundation for assessing, in future studies, the evolutionary path that led to the present-day variability of the venoms of palm-pitvipers.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-01
dc.date.accessioned.none.fl_str_mv 2018-06-07T19:17:27Z
dc.date.available.none.fl_str_mv 2018-06-07T19:17:27Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1874391916304444?via%3Dihub#!
dc.identifier.issn.none.fl_str_mv 1874-3919
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/74866
dc.identifier.doi.none.fl_str_mv 10.1016/j.jprot.2016.10.006
url https://www.sciencedirect.com/science/article/pii/S1874391916304444?via%3Dihub#!
https://hdl.handle.net/10669/74866
identifier_str_mv 1874-3919
10.1016/j.jprot.2016.10.006
dc.language.iso.es_ES.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
eu_rights_str_mv embargoedAccess
dc.source.es_ES.fl_str_mv Journal of Proteomics, Vol 152, pp 1-12
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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spelling Pla Ferrer, Davinia4dd10ec7-e6df-49e1-b953-079d9993b8ce600Sanz, Libiaa71fb767-584b-409f-b843-18c1977e6747600Sasa Marín, Mahmoode0a00694-ced7-4f73-85fc-cb7e5e4f9616600Acevedo, Manuel E.4e6756c3-7ade-4602-b667-5134109d7edaDwyer, Quetzale9be95d6-5ad5-4e29-8111-4da86db11af0Durban, Jordi9a6002b1-6392-444f-beb7-d2667ce429be600Pérez, Alicia450f12d8-0d51-4b39-9eac-20ce82fde9ea600Rodríguez, Yaniaa5ed9e86-a126-43ac-82ea-478e3ebdd52eLomonte, Bruno581484ad-aaa8-46b1-bc0e-f380d895640c600Calvete Chornet, Juan José03718753-da20-4f77-b16a-f5867f73b0db6002018-06-07T19:17:27Z2018-06-07T19:17:27Z2017-01https://www.sciencedirect.com/science/article/pii/S1874391916304444?via%3Dihub#!1874-3919https://hdl.handle.net/10669/7486610.1016/j.jprot.2016.10.006Bothriechis is a genus of eleven currently recognized slender and arboreal venomous snakes, commonly called palm-pitvipers that range from southern Mexico to northern South America. Despite dietary studies suggesting that palm-pitvipers are generalists with an ontogenetic shift toward endothermic prey, venom proteomic analyses have revealed remarkable divergence between the venoms of the Costa Rican species, B. lateralis, B. schlegelii, B. supraciliaris, and B. nigroviridis. To achieve a more complete picture of the venomic landscape across Bothriechis, the venom proteomes of biodiversity of the northern Middle American highland palm-pitvipers, B. thalassinus, B. aurifer, andB. bicolor fromGuatemala, B. marchi fromHonduras, and neonate Costa Rican B. lateralis and B. schlegelii, were investigated. B. thalassinus and B. aurifer venoms are comprised by similar toxin arsenals dominated by SVMPs (33–39% of the venom proteome), CTLs (11–16%), BPP-like molecules (10–13%), and CRISPs (5–10%), and are characterized by the absence of PLA2 proteins. Conversely, the predominant (35%) components of B. bicolor are D49-PLA2 molecules. The venom proteome of B. marchi is similar to B. aurifer and B. thalassinus in that it is rich in SVMPs and BPPs, but also contains appreciable amounts (14.3%) of PLA2s. The major toxin family found in the venoms of both neonate B. lateralis and B. schlegelii, is serine proteinase (SVSP), comprising about 20% of their toxin arsenals. The venomof neonate B. schlegelii is the only palm-pitviper venom where relative high amounts of Kunitz-type (6.3%) and γPLA2 (5.2%) inhibitors have been identified. Despite notable differences between their proteomes, neonate venoms are more similar to each other than to adults of their respective species. However, the ontogenetic changes taking place in the venom of B. lateralis strongly differ from those that occur in the venom of B. schlegelii. Thus, the ontogenetic change in B. lateralis produces a SVMP-rich venom, whereas in B. schlegelii the age-dependent compositional shift generates a PLA2-rich venom. Overall, genus-wide venomics illustrate the high evolvability of palm-pitviper venoms. The integration of the pattern of venom variation across Bothriechis into a phylogenetic and biogeographic framework may lay the foundation for assessing, in future studies, the evolutionary path that led to the present-day variability of the venoms of palm-pitvipers.Ministerio de Economía y Competitividad/[BFU2010-17373]//EspañaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)en_USacceso embargadohttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessJournal of Proteomics, Vol 152, pp 1-12reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRVenomicsGenus BothriechisArboreal palm-pitvipersBothriechis bicolorBothriechis aurifeBothriechis thalassinusBothriechis marchiNeonate B. schlegeliiNeonate B. lateralisSnake venom571.95 ToxicologíaProteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)artículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleORIGINAL2017_Prot_JProteomics_Pla_Bothriechis_variability_ontogeny.pdf2017_Prot_JProteomics_Pla_Bothriechis_variability_ontogeny.pdfVersión finalapplication/pdf3236200https://www.kerwa.ucr.ac.cr/bitstreams/0528d981-863c-43c6-ae02-c57a739ed71c/download049561b352e916d66e213b602cd09977MD51LICENSElicense.txtlicense.txttext/plain; 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